Restriction map for pF1KB6727
##################################
# NO CUT ENZYMES (  181 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AfaI        AflII       AflIII      
AgeI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BmyI        BpmI        BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CfoI        
Cfr13I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HhaI        HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        KspI        MaeI        MaeII       MaeIII      
MamI        MfeI        MluI        MroI        MseI        
MslI        MspA1I      MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TaqI        TfiI        Tru9I       
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AcyI            3  GGCGTC               G[GA]^CG[CT]C
AhdI           94  GACTACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI          244  AGCT                 AG^CT
AlwNI         100  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI          94  GACTACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         134  GGATCC               G^GATCC
BcgI          152  GCATCCTCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          131  CCGGG                CC^[GC]GG
BlpI          165  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      165  GCTGAGC              GC^T[ACGT]AGC
BsaHI           3  GGCGTC               G[GA]^CG[CT]C
BsaI           37  GGTCTC               GGTCTC(1/5)
BsmBI           5  CGTCTC               CGTCTC(1/5)
BsmFI         102  GTCCC                GGGAC(10/14)
BsrFI         179  GCCGGT               [GA]^CCGG[CT]
BstAPI        170  GCAGAAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         147  CCTGG                CC^[AT]GG
BstYI         134  GGATCC               [GA]^GATC[CT]
Cac8I         269  GCAGGC               GC[ACGT]^[ACGT]GC
CelII         165  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        179  GCCGGT               [GA]^CCGG[CT]
DdeI          166  CTGAG                C^T[ACGT]AG
DsaI           47  CCATGG               C^C[GA][CT]GG
Eam1105I       94  GACTACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII        147  CCTGG                ^CC[AT]GG
FauI          266  CCCGC                CCCGC(4/6)
HgaI            4  GCGTC                GACGC(5/10)
Hin1I           3  GGCGTC               G[GA]^CG[CT]C
HphI           44  TCACC                GGTGA(8/7)
MflI          134  GGATCC               [GA]^GATC[CT]
MvaI          147  CCTGG                CC^[AT]GG
NciI          131  CCGGG                CC^[GC]GG
NcoI           47  CCATGG               C^CATGG
NlaIII         48  CATG                 CATG^
PflMI          15  CCACATCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          106  CTGCAG               CTGCA^G
SfcI          106  CTGCAG               C^T[GA][CT]AG
StuI          117  AGGCCT               AGG^CCT
Van91I         15  CCACATCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         134  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AlwI          135  GATCC                GGATC(4/5)
              134  GGATC               
BalI           12  TGGCCA               TGG^CCA
               50  TGGCCA              
BbvI          198  GCAGC                GCAGC(8/12)
              242  GCAGC               
BseRI          40  CTCCTC               GAGGAG(10/8)
              226  GAGGAG              
BsmAI           6  GTCTC                GTCTC(1/5)
               38  GTCTC               
BsrI          208  ACTGG                ACTGG(1/-1)
               99  CCAGT               
BssKI         131  CCGGG                ^CC[ACGT]GG
              147  CCTGG               
DpnII         111  GATC                 ^GATC
              135  GATC                
EaeI           12  TGGCCA               [CT]^GGCC[GA]
               50  TGGCCA              
EarI          139  CTCTTC               CTCTTC(1/4)
              223  GAAGAG              
EcoT14I        47  CCATGG               C^C[AT][AT]GG
               53  CCAAGG              
Fnu4HI        198  GCAGC                GC^[ACGT]GC
              242  GCAGC               
HapII         131  CCGG                 C^CGG
              180  CCGG                
HpaII         131  CCGG                 C^CGG
              180  CCGG                
ItaI          198  GCAGC                GC^[ACGT]GC
              242  GCAGC               
Ksp632I       139  CTCTTC               CTCTTC(1/4)
              223  GAAGAG              
MboI          111  GATC                 ^GATC
              135  GATC                
MluNI          12  TGGCCA               TGG^CCA
               50  TGGCCA              
MscI           12  TGGCCA               TGG^CCA
               50  TGGCCA              
MspI          131  CCGG                 C^CGG
              180  CCGG                
MwoI          170  GCAGAAGATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              245  GCTCCATCCGC         
NdeII         111  GATC                 ^GATC
              135  GATC                
NlaIV         134  GGATCC               GG[ACGT]^[ACGT]CC
              213  GGAACC              
Sau3AI        111  GATC                 ^GATC
              135  GATC                
ScrFI         131  CCGGG                CC^[ACGT]GG
              147  CCTGG               
SfaNI         176  GATGC                GCATC(5/9)
              152  GCATC               
StyI           47  CCATGG               C^C[AT][AT]GG
               53  CCAAGG              
TseI          198  GCAGC                G^C[AT]GC
              242  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (    9 enzymes) #
#########################################

AciI        BsaJI       BsiYI       BslI        BstF5I      
FokI        HaeIII      MboII       MnlI        
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