Restriction map for pF1KB6727
 (Circular) (Six-base) MAPSORT of: pF1KB6727.seq  Check: 4484  from: 1  to: 3389



pF1KB6727  3389 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AccI GT'mk_AC

Cuts at:    427     427 
   Size:       3389
 
Acc65I G'GTAC_C

Cuts at:    405     405 
   Size:       3389
 
AclI AA'CG_TT

Cuts at:   3229    3229 
   Size:       3389
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1214    1646    2393    2661    1214 
   Size:        432     747     268    1942

  Fragments arranged by size: 

                1942     747     432     268
 
AflIII A'CryG_T

Cuts at:    741    1845    2822     741 
   Size:       1104     977    1308

  Fragments arranged by size: 

                1308    1104     977
 
AgeI A'CCGG_T

Cuts at:   1777    1777 
   Size:       3389
 
AhdI GACnn_n'nnGTC

Cuts at:    207     207 
   Size:       3389
 
AlwNI CAG_nnn'CTG

Cuts at:    213     694    2261     213 
   Size:        481    1567    1341

  Fragments arranged by size: 

                1567    1341     481
 
ApaLI G'TGCA_C

Cuts at:   2159    2159 
   Size:       3389
 
ApoI r'AATT_y

Cuts at:    393    2742    2998     393 
   Size:       2349     256     784

  Fragments arranged by size: 

                2349     784     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3389
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3389
 
AvaI C'yCGr_G

Cuts at:    409     409 
   Size:       3389
 
BamHI G'GATC_C

Cuts at:    242     414     242 
   Size:        172    3217
 
BanI G'GyrC_C

Cuts at:    405     819    1067    1102     405 
   Size:        414     248      35    2692

  Fragments arranged by size: 

                2692     414     248      35
 
BanII G_rGCy'C

Cuts at:    403    1433     403 
   Size:       1030    2359
 
BbeI G_GCGC'C

Cuts at:    823    1071     823 
   Size:        248    3141
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    249     283     249 
   Size:         34    3355
 
BciVI GTATCCnnnnn_n'

Cuts at:   1287    2048    3389    1287 
   Size:        761    1341    1287

  Fragments arranged by size: 

                1341    1287     761
 
BclI T'GATC_A

Cuts at:    909     909 
   Size:       3389
 
BglII A'GATC_T

Cuts at:    904    2941     904 
   Size:       2037    1352
 
BlpI GC'TnA_GC

Cuts at:    274     501     274 
   Size:        227    3162
 
Bme1580I G_kGCm'C

Cuts at:   1014    1107    2163    1014 
   Size:         93    1056    2240

  Fragments arranged by size: 

                2240    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    326     601     682    1017    3161     326 
   Size:        275      81     335    2144     554

  Fragments arranged by size: 

                2144     554     335     275      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1756    1756 
   Size:       3389
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    565    1444    1936    2234     565 
   Size:        879     492     298    1720

  Fragments arranged by size: 

                1720     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    151    3093     151 
   Size:       2942     447
 
BsaAI yAC'GTr

Cuts at:    742     742 
   Size:       3389
 
BsaBI GATnn'nnATC

Cuts at:      6     903    2940       6 
   Size:        897    2037     455

  Fragments arranged by size: 

                2037     897     455
 
BsaHI Gr'CG_yC

Cuts at:    112     820    1068    1770    3266     112 
   Size:        708     248     702    1496     235

  Fragments arranged by size: 

                1496     708     702     248     235
 
BsaWI w'CCGG_w

Cuts at:    578    1099    1777    2051    2198    2609     578 
   Size:        521     678     274     147     411    1358

  Fragments arranged by size: 

                1358     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    215     245     215 
   Size:         30    3359
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    139     349     139 
   Size:        210    3179
 
BseYI C'CCAG_C

Cuts at:    814    1572    2149    2645     814 
   Size:        758     577     496    1558

  Fragments arranged by size: 

                1558     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     931     977    2185     104 
   Size:        827      46    1208    1308

  Fragments arranged by size: 

                1308    1208     827      46
 
BsiHKAI G_wGCw'C

Cuts at:    403    1181    1371    2163     403 
   Size:        778     190     792    1629

  Fragments arranged by size: 

                1629     792     778     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    119     119 
   Size:       3389
 
Bsp1286I G_dGCh'C

Cuts at:    403    1014    1107    1181    1371    1433    2163     403 
   Size:        611      93      74     190      62     730    1629

  Fragments arranged by size: 

                1629     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2609    2609 
   Size:       3389
 
BspHI T'CATG_A

Cuts at:   2565    2565 
   Size:       3389
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    439     653     955    1336     439 
   Size:        214     302     381    2492

  Fragments arranged by size: 

                2492     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1681    1735    3219    3384      50 
   Size:       1631      54    1484     165      55

  Fragments arranged by size: 

                1631    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1301    1301 
   Size:       3389
 
BsrFI r'CCGG_y

Cuts at:    287    1387    1568    1777     287 
   Size:       1100     181     209    1899

  Fragments arranged by size: 

                1899    1100     209     181
 
BssSI C'ACGA_G

Cuts at:   1660    2018    1660 
   Size:        358    3031
 
BstAPI GCAn_nnn'nTGC

Cuts at:    284     766     284 
   Size:        482    2907
 
BstBI TT'CG_AA

Cuts at:   1750    1750 
   Size:       3389
 
BstYI r'GATC_y

Cuts at:      1     242     414     881     904    1239    1485    2497    2583 
   Size:        241     172     467      23     335     246    1012      86

Cuts at:   2583    2595    2941       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     246     241     172
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     155     107 
   Size:         48    3341
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    219    1339    1520    1683     219 
   Size:       1120     181     163    1925

  Fragments arranged by size: 

                1925    1120     181     163
 
BtsI GCAGTG_nn'

Cuts at:    632     632 
   Size:       3389
 
DraI TTT'AAA

Cuts at:    388     388 
   Size:       3389
 
DrdI GACnn_nn'nnGTC

Cuts at:   1095    1953    1095 
   Size:        858    2531
 
EaeI y'GGCC_r

Cuts at:    120     158     974    1148    1539    1566    2911     120 
   Size:         38     816     174     391      27    1345     598

  Fragments arranged by size: 

                1345     816     598     391     174      38      27
 
EagI C'GGCC_G

Cuts at:    974     974 
   Size:       3389
 
EarI CTCTTCn'nnn_

Cuts at:    253     326    1412    1622     253 
   Size:         73    1086     210    2020

  Fragments arranged by size: 

                2020    1086     210      73
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    349    1597    1907    2053    3199     349 
   Size:       1248     310     146    1146     539

  Fragments arranged by size: 

                1248    1146     539     310     146
 
EcoICRI GAG'CTC

Cuts at:    401     401 
   Size:       3389
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1214    1646    2393    2661    1214 
   Size:        432     747     268    1942

  Fragments arranged by size: 

                1942     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    528     528 
   Size:       3389
 
EcoRI G'AATT_C

Cuts at:    393     393 
   Size:       3389
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1408    1408 
   Size:       3389
 
FspI TGC'GCA

Cuts at:   1170    1170 
   Size:       3389
 
HaeII r_GCGC'y

Cuts at:    823    1071    2093    3072     823 
   Size:        248    1022     979    1140

  Fragments arranged by size: 

                1140    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    193     215     225     247    1231    1261    1263    1293     193 
   Size:         22      10      22     984      30       2      30    2289

  Fragments arranged by size: 

                2289     984      30      30      22      22      10       2
            
 
HincII GTy'rAC

Cuts at:    428     428 
   Size:       3389
 
KasI G'GCGC_C

Cuts at:    819    1067     819 
   Size:        248    3141
 
KpnI G_GTAC'C

Cuts at:    409     409 
   Size:       3389
 
MluI A'CGCG_T

Cuts at:   2822    2822 
   Size:       3389
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2060    2244    2060 
   Size:        184    3205
 
MscI TGG'CCA

Cuts at:    122     160    1150     122 
   Size:         38     990    2361

  Fragments arranged by size: 

                2361     990      38
 
MslI CAynn'nnrTG

Cuts at:   1378    1505    1378 
   Size:        127    3262
 
MspA1I CmG'CkG

Cuts at:    500     814    1174    2187    2432     500 
   Size:        314     360    1013     245    1457

  Fragments arranged by size: 

                1457    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1570    1570 
   Size:       3389
 
NarI GG'CG_CC

Cuts at:    820    1068     820 
   Size:        248    3141
 
NcoI C'CATG_G

Cuts at:    107     155     107 
   Size:         48    3341
 
NgoMIV G'CCGG_C

Cuts at:   1568    1568 
   Size:       3389
 
NspI r_CATG'y

Cuts at:    442     942    1849     442 
   Size:        500     907    1982

  Fragments arranged by size: 

                1982     907     500
 
PciI A'CATG_T

Cuts at:   1845    1845 
   Size:       3389
 
PflMI CCAn_nnn'nTGG

Cuts at:    129     129 
   Size:       3389
 
PmeI GTTT'AAAC

Cuts at:    388     388 
   Size:       3389
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2528    2560    2528 
   Size:         32    3357
 
PstI C_TGCA'G

Cuts at:    218     436    1121     218 
   Size:        218     685    2486

  Fragments arranged by size: 

                2486     685     218
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3389
 
PvuII CAG'CTG

Cuts at:    814    1174     814 
   Size:        360    3029
 
RsrII CG'GwC_CG

Cuts at:   1584    1584 
   Size:       3389
 
SacI G_AGCT'C

Cuts at:    403     403 
   Size:       3389
 
SalI G'TCGA_C

Cuts at:    426     426 
   Size:       3389
 
SapI GCTCTTCn'nnn_

Cuts at:   1412    1622    1412 
   Size:        210    3179
 
SbfI CC_TGCA'GG

Cuts at:    436     436 
   Size:       3389
 
ScaI AGT'ACT

Cuts at:   2906    2906 
   Size:       3389
 
SfcI C'TryA_G

Cuts at:     33     214     432    1117    2110    2301      33 
   Size:        181     218     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     218     191     181
 
SfoI GGC'GCC

Cuts at:    821    1069     821 
   Size:        248    3141
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3389
 
SmaI CCC'GGG

Cuts at:    411     411 
   Size:       3389
 
SmlI C'TyrA_G

Cuts at:    544    1459    1951    2213     544 
   Size:        915     492     262    1720

  Fragments arranged by size: 

                1720     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3389
 
SphI G_CATG'C

Cuts at:    442     942     442 
   Size:        500    2889
 
StuI AGG'CCT

Cuts at:    227     227 
   Size:       3389
 
StyI C'CwwG_G

Cuts at:    107     155     161     523     877     107 
   Size:         48       6     362     354    2619

  Fragments arranged by size: 

                2619     362     354      48       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    972    1105    1564    1773     972 
   Size:        133     459     209    2588

  Fragments arranged by size: 

                2588     459     209     133
 
TatI w'GTAC_w

Cuts at:   2904    2904 
   Size:       3389
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2906    2938    2906 
   Size:         32    3357
 
Tth111I GACn'n_nGTC

Cuts at:   1186    1186 
   Size:       3389
 
XbaI T'CTAG_A

Cuts at:    420     420 
   Size:       3389
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    884     884 
   Size:       3389
 
XmaI C'CCGG_G

Cuts at:    409     409 
   Size:       3389
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI    PflMI
     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI    BmgBI
     BmtI     BpmI   Bpu10I     BsgI    BsiWI     BsmI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PfoI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI
     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI
     ZraI
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