Restriction map for pF1KE0421
##################################
# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        AlwNI       
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbsI        BcgI        
BciVI       BclI        BcnI        BfrI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       Hin1I       
HindIII     HpaI        KasI        KpnI        Ksp632I     
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MunI        MvaI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NlaIV       NotI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        ScrFI       SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        Tru9I       Tth111I     TthHB8I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AfaI          378  GTAC                 GT^AC
AflIII         84  ACGTGT               A^C[GA][CT]GT
Alw44I        285  GTGCAC               G^TGCAC
ApaLI         285  GTGCAC               G^TGCAC
AspHI         285  GTGCAC               G[AT]GC[AT]^C
BbrPI          17  CACGTG               CAC^GTG
BbvCI         484  GCTGAGG              CCTCAGC(-5/-2)
BfaI          520  CTAG                 C^TAG
BglI          189  GCCATGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          285  GTGCAC               G[AGT]GC[ACT]^C
BseRI         487  GAGGAG               GAGGAG(10/8)
BsiHKAI       285  GTGCAC               G[AT]GC[AT]^C
BsmAI          98  GAGAC                GTCTC(1/5)
Bsp1286I      285  GTGCAC               G[AGT]GC[ACT]^C
BssHII         49  GCGCGC               G^CGCGC
BstXI         303  CCAGAAGGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI          116  TGGCCG               [CT]^GGCC[GA]
EcoT14I       190  CCATGG               C^C[AT][AT]GG
HapII          23  CCGG                 C^CGG
HgaI          121  GCGTC                GACGC(5/10)
HincII        388  GTTGAC               GT[CT]^[GA]AC
HindII        388  GTTGAC               GT[CT]^[GA]AC
HpaII          23  CCGG                 C^CGG
HphI          107  TCACC                GGTGA(8/7)
KspI           59  CCGCGG               CCGC^GG
MaeI          520  CTAG                 C^TAG
MslI          108  CACCGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           23  CCGG                 C^CGG
NcoI          190  CCATGG               C^CATGG
NruI           94  TCGCGA               TCG^CGA
PflMI          30  CCAAGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          154  GAGTC                GAGTC(4/5)
PmaCI          17  CACGTG               CAC^GTG
PmlI           17  CACGTG               CAC^GTG
RsaI          378  GTAC                 GT^AC
SacII          59  CCGCGG               CCGC^GG
SfaNI         562  GCATC                GCATC(5/9)
StyI          190  CCATGG               C^C[AT][AT]GG
TfiI          217  GAATC                G^A[AT]TC
Van91I         30  CCAAGTGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          255  CCAGCTGTTCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcsI          147  AAATTT               [GA]^AATT[CT]
              172  GAATTT              
AlwI           76  GATCC                GGATC(4/5)
              441  GATCC               
ApoI          147  AAATTT               [GA]^AATT[CT]
              172  GAATTT              
AvaII         162  GGACC                G^G[AT]CC
              223  GGACC               
BsaAI          17  CACGTG               [CT]AC^GT[GA]
               83  TACGTG              
BsaJI          59  CCGCGG               C^C[ACGT][ACGT]GG
              190  CCATGG              
BstF5I        563  CATCC                GGATG(2/0)
              280  GGATG               
BstYI          75  AGATCC               [GA]^GATC[CT]
              440  AGATCC              
Cac8I          49  GCGCGC               GC[ACGT]^[ACGT]GC
              360  GCTTGC              
Cfr13I        162  GGACC                G^G[ACGT]CC
              223  GGACC               
DpnII          76  GATC                 ^GATC
              441  GATC                
DsaI           59  CCGCGG               C^C[GA][CT]GG
              190  CCATGG              
FokI          563  CATCC                GGATG(9/13)
              280  GGATG               
HaeIII         63  GGCC                 GG^CC
              117  GGCC                
HinfI         154  GAGTC                G^A[ACGT]TC
              217  GAATC               
MaeII          18  ACGT                 A^CGT
               84  ACGT                
MboI           76  GATC                 ^GATC
              441  GATC                
MflI           75  AGATCC               [GA]^GATC[CT]
              440  AGATCC              
NdeII          76  GATC                 ^GATC
              441  GATC                
Sau3AI         76  GATC                 ^GATC
              441  GATC                
Sau96I        162  GGACC                G^G[ACGT]CC
              223  GGACC               
Tsp45I        156  GTCAC                ^GT[GC]AC
              274  GTCAC               
Tsp509I       148  AATT                 ^AATT
              173  AATT                
TspRI         158  CACTG                CA[GC]TG[ACGT][ACGT]^
              276  CACTG               
XhoII          75  AGATCC               [GA]^GATC[CT]
              440  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BsiYI       
BslI        BsrI        BstUI       CfoI        DdeI        
Fnu4HI      HhaI        HinP1I      ItaI        MaeIII      
MboII       MnlI        MspA1I      MvnI        MwoI        
NlaIII      PvuII       TseI        
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