Restriction map for pF1KE0434
##################################
# NO CUT ENZYMES (  158 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BcnI        BfaI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmFI       BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstUI       BstZ17I     CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DsaI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoRI       EcoRV       EcoT22I     FauI        FseI        
FspI        HaeII       HapII       HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MfeI        MluI        MroI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
TaqI        Tth111I     TthHB8I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AciI          100  GCGG                 CCGC(-3/-1)
AlwNI         221  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         940  GAGCAC               G[AT]GC[AT]^C
BalI          872  TGGCCA               TGG^CCA
BamHI         714  GGATCC               G^GATCC
BanI          186  GGTGCC               G^G[CT][GA]CC
BbsI           46  GTCTTC               GAAGAC(2/6)
BclI          660  TGATCA               T^GATCA
BmyI          940  GAGCAC               G[AGT]GC[ACT]^C
BpmI          761  CTGGAG               CTGGAG(16/14)
BpuAI          46  GTCTTC               GAAGAC(2/6)
BsaBI         888  GATGGCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI           56  GAGACC               GGTCTC(1/5)
BsiHKAI       940  GAGCAC               G[AT]GC[AT]^C
BsiYI         503  CCAATGTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          503  CCAATGTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I      940  GAGCAC               G[AGT]GC[ACT]^C
BspHI         840  TCATGA               T^CATGA
BstAPI        211  GCAGAGATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        314  GGTTACC              G^GT[ACGT]ACC
BstPI         314  GGTTACC              G^GT[ACGT]ACC
BstXI         582  CCATAGCATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI           58  GACCAGCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          872  TGGCCA               [CT]^GGCC[GA]
EcoO65I       314  GGTTACC              G^GT[ACGT]ACC
FbaI          660  TGATCA               T^GATCA
HgaI          831  GACGC                GACGC(5/10)
MamI          888  GATGGCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         872  TGGCCA               TGG^CCA
MscI          872  TGGCCA               TGG^CCA
MseI          805  TTAA                 T^TAA
MslI          664  CATCGGGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI          858  GCATGC               [GA]CATG^[CT]
PflMI         503  CCAATGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          209  CTGCAG               CTGCA^G
RcaI          840  TCATGA               T^CATGA
ScaI          643  AGTACT               AGT^ACT
SmlI          380  CTCAAG               C^T[CT][GA]AG
SphI          858  GCATGC               GCATG^C
StuI          921  AGGCCT               AGG^CCT
Tru9I         805  TTAA                 T^TAA
Van91I        503  CCAATGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          196  AAATTT               [GA]^AATT[CT]
              780  GAATTT              
AfaI           13  GTAC                 GT^AC
              644  GTAC                
AocI          319  CCTGAGG              CC^T[ACGT]AGG
              439  CCTGAGG             
ApoI          196  AAATTT               [GA]^AATT[CT]
              780  GAATTT              
BglII          90  AGATCT               A^GATCT
              726  AGATCT              
BsmI          498  GAATGC               GAATGC(1/-1)
              880  GCATTC              
BssKI         137  CCTGG                ^CC[ACGT]GG
              696  CCAGG               
BstF5I        447  GGATG                GGATG(2/0)
              887  GGATG               
BstNI         137  CCTGG                CC^[AT]GG
              696  CCAGG               
Bsu36I        319  CCTGAGG              CC^T[ACGT]AGG
              439  CCTGAGG             
Eco81I        319  CCTGAGG              CC^T[ACGT]AGG
              439  CCTGAGG             
EcoRII        137  CCTGG                ^CC[AT]GG
              696  CCAGG               
EcoT14I        78  CCTTGG               C^C[AT][AT]GG
              543  CCAAGG              
FokI          447  GGATG                GGATG(9/13)
              887  GGATG               
MspA1I         33  CAGCTG               C[AC]G^C[GT]G
               61  CAGCTG              
MvaI          137  CCTGG                CC^[AT]GG
              696  CCAGG               
MwoI          211  GCAGAGATTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              493  GCCCTGAATGC         
PvuII          33  CAGCTG               CAG^CTG
               61  CAGCTG              
RsaI           13  GTAC                 GT^AC
              644  GTAC                
ScrFI         137  CCTGG                CC^[ACGT]GG
              696  CCAGG               
SfcI          209  CTGCAG               C^T[GA][CT]AG
              467  CTACAG              
StyI           78  CCTTGG               C^C[AT][AT]GG
              543  CCAAGG              
TspRI         299  CACTG                CA[GC]TG[ACGT][ACGT]^
              759  CACTG               
XcmI           69  CCAACATACCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              719  CCATGTTAGATCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AluI        AlwI        AvaII       BbvI        BsaJI       
BseRI       BsmAI       BsrI        BstYI       Cac8I       
Cfr13I      DdeI        DpnII       Fnu4HI      HaeIII      
HinfI       HphI        ItaI        MaeIII      MboI        
MboII       MflI        MnlI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      SfaNI       TfiI        
TseI        Tsp45I      Tsp509I     XhoII       
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