Restriction map for pF1KE0434
 (Linear) (Six-base) MAPSORT of: KIEE0434.seq  Check: 4689  from: 1  to: 945



KIEE0434.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     945 
   Size:        374     571
 
AlwNI CAG_nnn'CTG

Cuts at:      0     226     945 
   Size:        226     719
 
ApoI r'AATT_y

Cuts at:      0     196     780     945 
   Size:        196     584     165

  Fragments arranged by size: 

                 584     196     165
 
BamHI G'GATC_C

Cuts at:      0     714     945 
   Size:        714     231
 
BanI G'GyrC_C

Cuts at:      0     186     945 
   Size:        186     759
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      39     945 
   Size:         39     906
 
BclI T'GATC_A

Cuts at:      0     660     945 
   Size:        660     285
 
BglII A'GATC_T

Cuts at:      0      90     726     945 
   Size:         90     636     219

  Fragments arranged by size: 

                 636     219      90
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     782     945 
   Size:        782     163
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     945 
   Size:        365     580
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      50     945 
   Size:         50     895
 
BsaBI GATnn'nnATC

Cuts at:      0     892     945 
   Size:        892      53
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     758     788     945 
   Size:        758      30     157

  Fragments arranged by size: 

                 758     157      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      30     276     445     457     779     782     945 
   Size:         30     246     169      12     322       3     163

  Fragments arranged by size: 

                 322     246     169     163      30      12       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     944     945 
   Size:        944       1
 
BsmI GAATG_Cn'

Cuts at:      0     504     880     945 
   Size:        504     376      65

  Fragments arranged by size: 

                 504     376      65
 
Bsp1286I G_dGCh'C

Cuts at:      0     944     945 
   Size:        944       1
 
BspHI T'CATG_A

Cuts at:      0     840     945 
   Size:        840     105
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     217     945 
   Size:        217     728
 
BstEII G'GTnAC_C

Cuts at:      0     314     945 
   Size:        314     631
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     589     945 
   Size:        589     356
 
BstYI r'GATC_y

Cuts at:      0      90     600     714     726     945 
   Size:         90     510     114      12     219

  Fragments arranged by size: 

                 510     219     114      90      12
 
Bsu36I CC'TnA_GG

Cuts at:      0     320     440     945 
   Size:        320     120     505

  Fragments arranged by size: 

                 505     320     120
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      64     945 
   Size:         64     881
 
EaeI y'GGCC_r

Cuts at:      0     872     945 
   Size:        872      73
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     782     945 
   Size:        374     408     163

  Fragments arranged by size: 

                 408     374     163
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     117     149     268     300     334     366     822     854 
   Size:        117      32     119      32      34      32     456      32

Cuts at:    854     945 
   Size:         91

  Fragments arranged by size: 

                 456     119     117      91      34      32      32      32
                  32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     945 
   Size:         93     852
 
MscI TGG'CCA

Cuts at:      0     874     945 
   Size:        874      71
 
MslI CAynn'nnrTG

Cuts at:      0     668     945 
   Size:        668     277
 
MspA1I CmG'CkG

Cuts at:      0      35      63     945 
   Size:         35      28     882

  Fragments arranged by size: 

                 882      35      28
 
NspI r_CATG'y

Cuts at:      0     862     945 
   Size:        862      83
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     509     945 
   Size:        509     436
 
PstI C_TGCA'G

Cuts at:      0     213     945 
   Size:        213     732
 
PvuII CAG'CTG

Cuts at:      0      35      63     945 
   Size:         35      28     882

  Fragments arranged by size: 

                 882      35      28
 
ScaI AGT'ACT

Cuts at:      0     645     945 
   Size:        645     300
 
SfcI C'TryA_G

Cuts at:      0     209     467     945 
   Size:        209     258     478

  Fragments arranged by size: 

                 478     258     209
 
SmlI C'TyrA_G

Cuts at:      0     380     945 
   Size:        380     565
 
SphI G_CATG'C

Cuts at:      0     862     945 
   Size:        862      83
 
StuI AGG'CCT

Cuts at:      0     923     945 
   Size:        923      22
 
StyI C'CwwG_G

Cuts at:      0      78     543     945 
   Size:         78     465     402

  Fragments arranged by size: 

                 465     402      78
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     626     945 
   Size:        626     319
 
TatI w'GTAC_w

Cuts at:      0     643     945 
   Size:        643     302
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     758     790     945 
   Size:        758      32     155

  Fragments arranged by size: 

                 758     155      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      76     726     945 
   Size:         76     650     219

  Fragments arranged by size: 

                 650     219      76
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    BamHI     BanI     BbsI     BclI    BglII
     BpmI    BpuEI     BsaI    BsaBI    BsaXI    BseRI  BsiHKAI     BsmI
 Bsp1286I    BspHI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     DrdI
     EaeI  Eco57MI    Hin4I     MmeI     MscI     MslI   MspA1I     NspI
    PflMI     PstI    PvuII     ScaI     SfcI     SmlI     SphI     StuI
     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BsaAI    BsaHI    BsaWI    BseYI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI  BstZ17I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com