Restriction map for pF1KB6806
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BbrPI       BbsI        
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BpmI        BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BsrI        BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        Eam1105I    
EarI        Eco47III    Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FokI        FspI        HgaI        HincII      HindII      
HindIII     HpaI        KpnI        Ksp632I     KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NcoI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tsp509I     Tth111I     
Van91I      XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcyI           16  GGCGCC               G[GA]^CG[CT]C
AluI          184  AGCT                 AG^CT
AlwNI         509  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          173  GGGCCC               GGGCC^C
AvaII          86  GGTCC                G^G[AT]CC
BamHI          97  GGATCC               G^GATCC
BanI           16  GGCGCC               G^G[CT][GA]CC
BanII         173  GGGCCC               G[GA]GC[CT]^C
BbeI           16  GGCGCC               GGCGC^C
BbvCI         111  GCTGAGG              CCTCAGC(-5/-2)
BglI           56  GCCCGGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          156  GCTGAGC              GC^T[ACGT]AGC
BmyI          173  GGGCCC               G[AGT]GC[ACT]^C
Bpu1102I      156  GCTGAGC              GC^T[ACGT]AGC
BsaHI          16  GGCGCC               G[GA]^CG[CT]C
BseRI         397  GAGGAG               GAGGAG(10/8)
BsgI           73  CTGCAC               GTGCAG(16/14)
BsmFI          87  GTCCC                GGGAC(10/14)
Bsp1286I      173  GGGCCC               G[AGT]GC[ACT]^C
BssHII         30  GCGCGC               G^CGCGC
BstNI         327  CCTGG                CC^[AT]GG
BstXI         530  CCATTGATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI          97  GGATCC               [GA]^GATC[CT]
CelII         156  GCTGAGC              GC^T[ACGT]AGC
DraII          85  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I       85  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRII        327  CCTGG                ^CC[AT]GG
FseI           60  GGCCGGCC             GGCCGG^CC
HaeII          16  GGCGCC               [GA]GCGC^[CT]
Hin1I          16  GGCGCC               G[GA]^CG[CT]C
HinfI         259  GATTC                G^A[ACGT]TC
KasI           16  GGCGCC               G^GCGCC
MaeIII        344  GTCAC                ^GT[ACGT]AC
MflI           97  GGATCC               [GA]^GATC[CT]
MseI          235  TTAA                 T^TAA
MvaI          327  CCTGG                CC^[AT]GG
NaeI           61  GCCGGC               GCC^GGC
NarI           16  GGCGCC               GG^CGCC
NdeI          422  CATATG               CA^TATG
NgoMIV         61  GCCGGC               G^CCGGC
PpuMI          85  GGGTCCC              [GA]G^G[AT]CC[CT]
SfiI           55  GGCCCGGCCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI           16  GGCGCC               GGC^GCC
TfiI          259  GATTC                G^A[AT]TC
Tru9I         235  TTAA                 T^TAA
Tsp45I        344  GTCAC                ^GT[GC]AC
XbaI          291  TCTAGA               T^CTAGA
XhoII          97  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AlwI           98  GATCC                GGATC(4/5)
               97  GGATC               
BfaI          268  CTAG                 C^TAG
              292  CTAG                
BsiYI          62  CCGGCCGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              120  CCGGGGGCCGG         
BslI           62  CCGGCCGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              120  CCGGGGGCCGG         
EaeI           59  CGGCCG               [CT]^GGCC[GA]
               63  CGGCCG              
EagI           59  CGGCCG               C^GGCCG
               63  CGGCCG              
EclXI          59  CGGCCG               C^GGCCG
               63  CGGCCG              
Eco52I         59  CGGCCG               C^GGCCG
               63  CGGCCG              
MaeI          268  CTAG                 C^TAG
              292  CTAG                
MboII         190  GAAGA                GAAGA(8/7)
              358  GAAGA               
MslI          364  CACATATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              531  CATTGATGTG          
MspA1I          8  CCGCTG               C[AC]G^C[GT]G
              102  CAGCGG              
NlaIII        314  CATG                 CATG^
              408  CATG                
NspI          313  GCATGT               [GA]CATG^[CT]
              407  ACATGC              
SfaNI         382  GATGC                GCATC(5/9)
              517  GATGC               
TaqI          413  TCGA                 T^CGA
              495  TCGA                
TspRI         390  CACTG                CA[GC]TG[ACGT][ACGT]^
              509  CAGTG               
TthHB8I       413  TCGA                 T^CGA
              495  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AccII       AciI        BbvI        BcnI        BsaJI       
BsiEI       BsrFI       BssKI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
FauI        Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MboI        
MnlI        MspI        MvnI        MwoI        NciI        
NdeII       NlaIV       Sau3AI      Sau96I      ScrFI       
TseI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com