Restriction map for pF1KB6461
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaI        
BseAI       BsgI        BsiHKAI     BsiWI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HpaI        KpnI        MaeI        
MfeI        MluI        MroI        MseI        MunI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsaI        
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
StuI        SwaI        Tru9I       Tth111I     XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccII         888  CGCG                 CG^CG
AflIII        482  ACACGT               A^C[GA][CT]GT
AlwI          500  GGATC                GGATC(4/5)
BalI          854  TGGCCA               TGG^CCA
BbeI          832  GGCGCC               GGCGC^C
BglI          644  GCCACACCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         354  AGATCT               A^GATCT
BsaBI         302  GATTGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         793  TCCGGT               [AT]^CCGG[AT]
BsmAI         190  GTCTC                GTCTC(1/5)
BsmBI         189  CGTCTC               CGTCTC(1/5)
BspMI         456  GCAGGT               ACCTGC(4/8)
BsrBI          95  CCGCTC               CCGCTC(-3/-3)
BsrFI         649  ACCGGC               [GA]^CCGG[CT]
BstAPI        479  GCAACACGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         888  CGCG                 CG^CG
BstXI         203  CCAAGAACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         354  AGATCT               [GA]^GATC[CT]
Cfr10I        649  ACCGGC               [GA]^CCGG[CT]
DraII         900  GGGACCT              [GA]G^G[ACGT]CC[CT]
EagI          927  CGGCCG               C^GGCCG
EarI          169  GAAGAG               CTCTTC(1/4)
EclXI         927  CGGCCG               C^GGCCG
Eco52I        927  CGGCCG               C^GGCCG
EcoO109I      900  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       896  CCATGG               C^C[AT][AT]GG
FauI          786  GCGGG                CCCGC(4/6)
HaeII         832  GGCGCC               [GA]GCGC^[CT]
HgaI          808  GACGC                GACGC(5/10)
HindIII       398  AAGCTT               A^AGCTT
KasI          832  GGCGCC               G^GCGCC
Ksp632I       169  GAAGAG               CTCTTC(1/4)
KspI          887  CCGCGG               CCGC^GG
MamI          302  GATTGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII         169  GAAGA                GAAGA(8/7)
MflI          354  AGATCT               [GA]^GATC[CT]
MluNI         854  TGGCCA               TGG^CCA
MscI          854  TGGCCA               TGG^CCA
MslI          220  CACCACGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          888  CGCG                 CG^CG
NarI          832  GGCGCC               GG^CGCC
NcoI          896  CCATGG               C^CATGG
PflMI         542  CCAAGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         900  GGGACCT              [GA]G^G[AT]CC[CT]
SacII         887  CCGCGG               CCGC^GG
SfcI          723  CTACAG               C^T[GA][CT]AG
SfoI          832  GGCGCC               GGC^GCC
SmlI          194  CTCAAG               C^T[CT][GA]AG
SphI          636  GCATGC               GCATG^C
SspI           14  AATATT               AAT^ATT
StyI          896  CCATGG               C^C[AT][AT]GG
TfiI          907  GATTC                G^A[AT]TC
Van91I        542  CCAAGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         354  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcyI          808  GACGCC               G[GA]^CG[CT]C
              832  GGCGCC              
BanI          538  GGCACC               G^G[CT][GA]CC
              832  GGCGCC              
BbrPI         483  CACGTG               CAC^GTG
              715  CACGTG              
BpmI          737  CTCCAG               CTGGAG(16/14)
              274  CTGGAG              
BsaAI         483  CACGTG               [CT]AC^GT[GA]
              715  CACGTG              
BsaHI         808  GACGCC               G[GA]^CG[CT]C
              832  GGCGCC              
BstEII         87  GGTGACC              G^GT[ACGT]ACC
              226  GGTGACC             
BstPI          87  GGTGACC              G^GT[ACGT]ACC
              226  GGTGACC             
CfoI          238  GCGC                 GCG^C
              833  GCGC                
EaeI          854  TGGCCA               [CT]^GGCC[GA]
              927  CGGCCG              
EcoO65I        87  GGTGACC              G^GT[ACGT]ACC
              226  GGTGACC             
HhaI          238  GCGC                 GCG^C
              833  GCGC                
Hin1I         808  GACGCC               G[GA]^CG[CT]C
              832  GGCGCC              
HinP1I        238  GCGC                 G^CGC
              833  GCGC                
NspI          636  GCATGC               [GA]CATG^[CT]
              859  ACATGC              
PmaCI         483  CACGTG               CAC^GTG
              715  CACGTG              
PmlI          483  CACGTG               CAC^GTG
              715  CACGTG              
SfaNI         783  GATGC                GCATC(5/9)
              599  GCATC               
TaqI           26  TCGA                 T^CGA
              285  TCGA                
Tsp509I       380  AATT                 ^AATT
              505  AATT                
TspRI         136  CACTG                CA[GC]TG[ACGT][ACGT]^
              547  CAGTG               
TthHB8I        26  TCGA                 T^CGA
              285  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AluI        AlwNI       AvaII       BanII       
BbvI        BcnI        BmyI        BsaJI       BseRI       
BsiEI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DsaI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PvuII       Sau3AI      Sau96I      ScrFI       
TseI        Tsp45I      
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