Restriction map for pF1KB6461
 (Linear) (Six-base) MAPSORT of: KIBB6461.seq  Check: 770  from: 1  to: 963



KIBB6461.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     447     963 
   Size:        447     516
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     386     703     963 
   Size:        386     317     260

  Fragments arranged by size: 

                 386     317     260
 
AflIII A'CryG_T

Cuts at:      0     482     963 
   Size:        482     481
 
AleI CACnn'nnGTG

Cuts at:      0     224     963 
   Size:        224     739
 
AlwNI CAG_nnn'CTG

Cuts at:      0     273     731     922     963 
   Size:        273     458     191      41

  Fragments arranged by size: 

                 458     273     191      41
 
BanI G'GyrC_C

Cuts at:      0     538     832     963 
   Size:        538     294     131

  Fragments arranged by size: 

                 538     294     131
 
BanII G_rGCy'C

Cuts at:      0     738     749     793     963 
   Size:        738      11      44     170

  Fragments arranged by size: 

                 738     170      44      11
 
BbeI G_GCGC'C

Cuts at:      0     836     963 
   Size:        836     127
 
BglI GCCn_nnn'nGGC

Cuts at:      0     650     963 
   Size:        650     313
 
BglII A'GATC_T

Cuts at:      0     354     963 
   Size:        354     609
 
Bme1580I G_kGCm'C

Cuts at:      0     490     541     963 
   Size:        490      51     422

  Fragments arranged by size: 

                 490     422      51
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     147     243     741     963 
   Size:        147      96     498     222

  Fragments arranged by size: 

                 498     222     147      96
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     722     963 
   Size:        295     427     241

  Fragments arranged by size: 

                 427     295     241
 
Bpu10I CC'TnA_GC

Cuts at:      0     442     963 
   Size:        442     521
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179     963 
   Size:        179     784
 
BsaAI yAC'GTr

Cuts at:      0     485     717     963 
   Size:        485     232     246

  Fragments arranged by size: 

                 485     246     232
 
BsaBI GATnn'nnATC

Cuts at:      0     306     963 
   Size:        306     657
 
BsaHI Gr'CG_yC

Cuts at:      0     809     833     963 
   Size:        809      24     130

  Fragments arranged by size: 

                 809     130      24
 
BsaWI w'CCGG_w

Cuts at:      0     793     963 
   Size:        793     170
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      33      63     637     667     963 
   Size:         33      30     574      30     296

  Fragments arranged by size: 

                 574     296      33      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      43     442     628     963 
   Size:         43     399     186     335

  Fragments arranged by size: 

                 399     335     186      43
 
BseYI C'CCAG_C

Cuts at:      0      78     106     828     963 
   Size:         78      28     722     135

  Fragments arranged by size: 

                 722     135      78      28
 
BsiEI CG_ry'CG

Cuts at:      0     798     822     930     963 
   Size:        798      24     108      33

  Fragments arranged by size: 

                 798     108      33      24
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     195     963 
   Size:        195     768
 
Bsp1286I G_dGCh'C

Cuts at:      0     490     541     738     749     793     963 
   Size:        490      51     197      11      44     170

  Fragments arranged by size: 

                 490     197     170      51      44      11
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     447     963 
   Size:        447     516
 
BsrBI CCG'CTC

Cuts at:      0      97     963 
   Size:         97     866
 
BsrFI r'CCGG_y

Cuts at:      0     649     963 
   Size:        649     314
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     485     963 
   Size:        485     478
 
BstEII G'GTnAC_C

Cuts at:      0      87     226     963 
   Size:         87     139     737

  Fragments arranged by size: 

                 737     139      87
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     210     963 
   Size:        210     753
 
BstYI r'GATC_y

Cuts at:      0     354     963 
   Size:        354     609
 
BtgI C'CryG_G

Cuts at:      0     222     887     896     963 
   Size:        222     665       9      67

  Fragments arranged by size: 

                 665     222      67       9
 
BtsI GCAGTG_nn'

Cuts at:      0     553     963 
   Size:        553     410
 
EaeI y'GGCC_r

Cuts at:      0     854     927     963 
   Size:        854      73      36

  Fragments arranged by size: 

                 854      73      36
 
EagI C'GGCC_G

Cuts at:      0     927     963 
   Size:        927      36
 
EarI CTCTTCn'nnn_

Cuts at:      0     164     963 
   Size:        164     799
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     386     703     722     963 
   Size:        295      91     317      19     241

  Fragments arranged by size: 

                 317     295     241      91      19
 
EcoO109I rG'GnC_Cy

Cuts at:      0     901     963 
   Size:        901      62
 
HaeII r_GCGC'y

Cuts at:      0     836     963 
   Size:        836     127
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      81     113     963 
   Size:         81      32     850

  Fragments arranged by size: 

                 850      81      32
 
HindIII A'AGCT_T

Cuts at:      0     398     963 
   Size:        398     565
 
KasI G'GCGC_C

Cuts at:      0     832     963 
   Size:        832     131
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     534     963 
   Size:        534     429
 
MscI TGG'CCA

Cuts at:      0     856     963 
   Size:        856     107
 
MslI CAynn'nnrTG

Cuts at:      0     224     963 
   Size:        224     739
 
MspA1I CmG'CkG

Cuts at:      0     165     467     591     852     889     963 
   Size:        165     302     124     261      37      74

  Fragments arranged by size: 

                 302     261     165     124      74      37
 
NarI GG'CG_CC

Cuts at:      0     833     963 
   Size:        833     130
 
NcoI C'CATG_G

Cuts at:      0     896     963 
   Size:        896      67
 
NspI r_CATG'y

Cuts at:      0     640     863     963 
   Size:        640     223     100

  Fragments arranged by size: 

                 640     223     100
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     548     963 
   Size:        548     415
 
PfoI T'CCnGG_A

Cuts at:      0     328     422     655     963 
   Size:        328      94     233     308

  Fragments arranged by size: 

                 328     308     233      94
 
PmlI CAC'GTG

Cuts at:      0     485     717     963 
   Size:        485     232     246

  Fragments arranged by size: 

                 485     246     232
 
PpuMI rG'GwC_Cy

Cuts at:      0     901     963 
   Size:        901      62
 
PvuII CAG'CTG

Cuts at:      0     165     591     852     963 
   Size:        165     426     261     111

  Fragments arranged by size: 

                 426     261     165     111
 
SacII CC_GC'GG

Cuts at:      0     890     963 
   Size:        890      73
 
SfcI C'TryA_G

Cuts at:      0     723     963 
   Size:        723     240
 
SfoI GGC'GCC

Cuts at:      0     834     963 
   Size:        834     129
 
SmlI C'TyrA_G

Cuts at:      0     194     963 
   Size:        194     769
 
SphI G_CATG'C

Cuts at:      0     640     963 
   Size:        640     323
 
SspI AAT'ATT

Cuts at:      0      16     963 
   Size:         16     947
 
StyI C'CwwG_G

Cuts at:      0     896     963 
   Size:        896      67
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     690     828     963 
   Size:        690     138     135

  Fragments arranged by size: 

                 690     138     135
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     637     669     963 
   Size:        637      32     294

  Fragments arranged by size: 

                 637     294      32
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI    AlwNI     BanI    BanII     BbeI
     BglI    BglII Bme1580I     BmrI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrFI   BstAPI   BstEII    BstXI    BstYI
     BtgI     BtsI     EaeI     EagI     EarI  Eco57MI EcoO109I    HaeII
    Hin4I  HindIII     KasI     MmeI     MscI     MslI   MspA1I     NarI
     NcoI     NspI    PflMI     PfoI     PmlI    PpuMI    PvuII    SacII
     SfcI     SfoI     SmlI     SphI     SspI     StyI    TaqII     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BlpI    BmgBI     BmtI     BplI     BsaI     BsgI
  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BsrDI    BsrGI   BssHII
    BssSI    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII     HpaI     KpnI     MfeI     MluI
     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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