Restriction map for pF1KB5050
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BanII       BbeI        BbrPI       BbvCI       
BcgI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BsrGI       BssHII      BssSI       BstAPI      BstUI       
CelII       CfoI        ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRI       FauI        FseI        FspI        
HaeII       HhaI        HinP1I      HindIII     KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        MvnI        NaeI        NarI        
NciI        NgoMIV      NheI        NotI        NruI        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SwaI        
Tth111I     Van91I      XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AcyI          425  GACGCC               G[GA]^CG[CT]C
AocI          877  CCTAAGG              CC^T[ACGT]AGG
Asp700I      1723  GAATTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          408  TGGCCA               TGG^CCA
BamHI        1338  GGATCC               G^GATCC
BanI         1496  GGCACC               G^G[CT][GA]CC
BbsI          706  GAAGAC               GAAGAC(2/6)
BciVI        1412  GTATCC               GTATCC(6/5)
BfaI         1738  CTAG                 C^TAG
BpuAI         706  GAAGAC               GAAGAC(2/6)
BsaHI         425  GACGCC               G[GA]^CG[CT]C
BseRI         860  GAGGAG               GAGGAG(10/8)
BsgI         1811  GTGCAG               GTGCAG(16/14)
BspHI        1167  TCATGA               T^CATGA
BspMI         659  ACCTGC               ACCTGC(4/8)
BsrBI         294  CCGCTC               CCGCTC(-3/-3)
BsrFI          63  GCCGGT               [GA]^CCGG[CT]
Bst1107I     1118  GTATAC               GTA^TAC
BstBI        1730  TTCGAA               TT^CGAA
BstEII        931  GGTAACC              G^GT[ACGT]ACC
BstF5I       1458  GGATG                GGATG(2/0)
BstPI         931  GGTAACC              G^GT[ACGT]ACC
BstXI        1844  CCAAGCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1118  GTATAC               GTA^TAC
Bsu36I        877  CCTAAGG              CC^T[ACGT]AGG
Cac8I         510  GCTTGC               GC[ACGT]^[ACGT]GC
Cfr10I         63  GCCGGT               [GA]^CCGG[CT]
DraI          966  TTTAAA               TTT^AAA
EaeI          408  TGGCCA               [CT]^GGCC[GA]
EarI          739  GAAGAG               CTCTTC(1/4)
Eco81I        877  CCTAAGG              CC^T[ACGT]AGG
EcoNI         136  CCTGAAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       931  GGTAACC              G^GT[ACGT]ACC
EcoRV        1579  GATATC               GAT^ATC
EcoT22I      1159  ATGCAT               ATGCA^T
FokI         1458  GGATG                GGATG(9/13)
HgaI          425  GACGC                GACGC(5/10)
Hin1I         425  GACGCC               G[GA]^CG[CT]C
Ksp632I       739  GAAGAG               CTCTTC(1/4)
MaeI         1738  CTAG                 C^TAG
MluNI         408  TGGCCA               TGG^CCA
MscI          408  TGGCCA               TGG^CCA
MspA1I       1272  CCGCTG               C[AC]G^C[GT]G
NsiI         1159  ATGCAT               ATGCA^T
NspV         1730  TTCGAA               TT^CGAA
PaeR7I       1193  CTCGAG               C^TCGAG
RcaI         1167  TCATGA               T^CATGA
SexAI         789  ACCAGGT              A^CC[AT]GGT
SfuI         1730  TTCGAA               TT^CGAA
SmlI         1193  CTCGAG               C^T[CT][GA]AG
SspI         1534  AATATT               AAT^ATT
StuI          456  AGGCCT               AGG^CCT
XcmI         1416  CCATGTGGTGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1193  CTCGAG               C^TCGAG
XmnI         1723  GAATTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI         1118  GTATAC               GT^[AC][GT]AC
             1358  GTCTAC              
AcsI          695  GAATTT               [GA]^AATT[CT]
             1723  GAATTT              
AfaI          344  GTAC                 GT^AC
              358  GTAC                
ApoI          695  GAATTT               [GA]^AATT[CT]
             1723  GAATTT              
AvaI            9  CCCGAG               C^[CT]CG[GA]G
             1193  CTCGAG              
BclI          900  TGATCA               T^GATCA
              908  TGATCA              
BmyI          186  GGGCAC               G[AGT]GC[ACT]^C
             1495  GGGCAC              
BsoBI           9  CCCGAG               C^[CT]CG[GA]G
             1193  CTCGAG              
Bsp1286I      186  GGGCAC               G[AGT]GC[ACT]^C
             1495  GGGCAC              
BspLU11I      679  ACATGT               A^CATGT
             1242  ACATGT              
DraII         389  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1614  AGGTCCC             
DsaI          279  CCATGG               C^C[GA][CT]GG
              808  CCATGG              
EcoO109I      389  GGGACCT              [GA]G^G[ACGT]CC[CT]
             1614  AGGTCCC             
FbaI          900  TGATCA               T^GATCA
              908  TGATCA              
HapII          64  CCGG                 C^CGG
              174  CCGG                
HincII       1488  GTTAAC               GT[CT]^[GA]AC
             1506  GTTAAC              
HindII       1488  GTTAAC               GT[CT]^[GA]AC
             1506  GTTAAC              
HpaI         1488  GTTAAC               GTT^AAC
             1506  GTTAAC              
HpaII          64  CCGG                 C^CGG
              174  CCGG                
HphI         1422  GGTGA                GGTGA(8/7)
             1720  GGTGA               
MspI           64  CCGG                 C^CGG
              174  CCGG                
NcoI          279  CCATGG               C^CATGG
              808  CCATGG              
NdeI          559  CATATG               CA^TATG
             1313  CATATG              
PleI         1388  GAGTC                GAGTC(4/5)
             1821  GAGTC               
PpuMI         389  GGGACCT              [GA]G^G[AT]CC[CT]
             1614  AGGTCCC             
RsaI          344  GTAC                 GT^AC
              358  GTAC                
TaqI         1194  TCGA                 T^CGA
             1731  TCGA                
TthHB8I      1194  TCGA                 T^CGA
             1731  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AflIII      AluI        AlwI        AlwNI       
AvaII       BbvI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsrDI       
BsrI        BssKI       BstNI       BstYI       Cfr13I      
DdeI        DpnII       EcoRII      EcoT14I     Fnu4HI      
HaeIII      HinfI       ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com