Restriction map for pF1KB5050
 (Linear) (Six-base) MAPSORT of: KIBB5050.seq  Check: 786  from: 1  to: 1881



KIBB5050.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AccI GT'mk_AC

Cuts at:      0    1119    1359    1881 
   Size:       1119     240     522

  Fragments arranged by size: 

                1119     522     240
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     725    1856    1881 
   Size:        220     505    1131      25

  Fragments arranged by size: 

                1131     505     220      25
 
AflIII A'CryG_T

Cuts at:      0     679    1242    1364    1484    1881 
   Size:        679     563     122     120     397

  Fragments arranged by size: 

                 679     563     397     122     120
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1649    1881 
   Size:       1649     232
 
AlwNI CAG_nnn'CTG

Cuts at:      0     162    1108    1348    1881 
   Size:        162     946     240     533

  Fragments arranged by size: 

                 946     533     240     162
 
ApoI r'AATT_y

Cuts at:      0     695    1723    1881 
   Size:        695    1028     158

  Fragments arranged by size: 

                1028     695     158
 
AvaI C'yCGr_G

Cuts at:      0       9    1193    1881 
   Size:          9    1184     688

  Fragments arranged by size: 

                1184     688       9
 
BamHI G'GATC_C

Cuts at:      0    1338    1881 
   Size:       1338     543
 
BanI G'GyrC_C

Cuts at:      0    1496    1881 
   Size:       1496     385
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     713    1881 
   Size:        713    1168
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1423    1881 
   Size:       1423     458
 
BclI T'GATC_A

Cuts at:      0     900     908    1881 
   Size:        900       8     973

  Fragments arranged by size: 

                 973     900       8
 
BfrBI ATG'CAT

Cuts at:      0    1161    1881 
   Size:       1161     720
 
Bme1580I G_kGCm'C

Cuts at:      0     190    1499    1881 
   Size:        190    1309     382

  Fragments arranged by size: 

                1309     382     190
 
BmgBI CAC'GTC

Cuts at:      0      95    1881 
   Size:         95    1786
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     359     576    1881 
   Size:        359     217    1305

  Fragments arranged by size: 

                1305     359     217
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     312     883    1448    1881 
   Size:        312     571     565     433

  Fragments arranged by size: 

                 571     565     433     312
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445     510     711     741     873    1130    1881 
   Size:        445      65     201      30     132     257     751

  Fragments arranged by size: 

                 751     445     257     201     132      65      30
 
Bpu10I CC'TnA_GC

Cuts at:      0     100    1881 
   Size:        100    1781
 
BsaHI Gr'CG_yC

Cuts at:      0     426    1881 
   Size:        426    1455
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     875    1881 
   Size:        875    1006
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1832    1881 
   Size:       1832      49
 
BsmI GAATG_Cn'

Cuts at:      0    1104    1478    1557    1881 
   Size:       1104     374      79     324

  Fragments arranged by size: 

                1104     374     324      79
 
Bsp1286I G_dGCh'C

Cuts at:      0     190    1499    1881 
   Size:        190    1309     382

  Fragments arranged by size: 

                1309     382     190
 
BspHI T'CATG_A

Cuts at:      0    1167    1881 
   Size:       1167     714
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     668    1881 
   Size:        668    1213
 
BsrBI CCG'CTC

Cuts at:      0     296    1881 
   Size:        296    1585
 
BsrDI GCAATG_nn'

Cuts at:      0     604     757    1606    1881 
   Size:        604     153     849     275

  Fragments arranged by size: 

                 849     604     275     153
 
BsrFI r'CCGG_y

Cuts at:      0      63    1881 
   Size:         63    1818
 
BstBI TT'CG_AA

Cuts at:      0    1731    1881 
   Size:       1731     150
 
BstEII G'GTnAC_C

Cuts at:      0     931    1881 
   Size:        931     950
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1851    1881 
   Size:       1851      30
 
BstYI r'GATC_y

Cuts at:      0    1069    1338    1429    1881 
   Size:       1069     269      91     452

  Fragments arranged by size: 

                1069     452     269      91
 
BstZ17I GTA'TAC

Cuts at:      0    1120    1881 
   Size:       1120     761
 
Bsu36I CC'TnA_GG

Cuts at:      0     878    1881 
   Size:        878    1003
 
BtgI C'CryG_G

Cuts at:      0     279     808    1881 
   Size:        279     529    1073

  Fragments arranged by size: 

                1073     529     279
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     336     371    1881 
   Size:        336      35    1510

  Fragments arranged by size: 

                1510     336      35
 
DraI TTT'AAA

Cuts at:      0     968    1881 
   Size:        968     913
 
EaeI y'GGCC_r

Cuts at:      0     408    1881 
   Size:        408    1473
 
EarI CTCTTCn'nnn_

Cuts at:      0     734    1881 
   Size:        734    1147
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1794    1881 
   Size:       1794      87
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     445     510     711     725     741     873    1130 
   Size:        220     225      65     201      14      16     132     257

Cuts at:   1130    1856    1881 
   Size:        726      25

  Fragments arranged by size: 

                 726     257     225     220     201     132      65      25
                  16      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     140    1881 
   Size:        140    1741
 
EcoO109I rG'GnC_Cy

Cuts at:      0     390    1615    1881 
   Size:        390    1225     266

  Fragments arranged by size: 

                1225     390     266
 
EcoRV GAT'ATC

Cuts at:      0    1581    1881 
   Size:       1581     300
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     730    1881 
   Size:        730    1151
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1040    1072    1881 
   Size:       1040      32     809

  Fragments arranged by size: 

                1040     809      32
 
HincII GTy'rAC

Cuts at:      0    1490    1508    1881 
   Size:       1490      18     373

  Fragments arranged by size: 

                1490     373      18
 
HpaI GTT'AAC

Cuts at:      0    1490    1508    1881 
   Size:       1490      18     373

  Fragments arranged by size: 

                1490     373      18
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     254    1804    1881 
   Size:        254    1550      77

  Fragments arranged by size: 

                1550     254      77
 
MscI TGG'CCA

Cuts at:      0     410    1881 
   Size:        410    1471
 
MslI CAynn'nnrTG

Cuts at:      0    1022    1166    1631    1881 
   Size:       1022     144     465     250

  Fragments arranged by size: 

                1022     465     250     144
 
MspA1I CmG'CkG

Cuts at:      0    1274    1881 
   Size:       1274     607
 
NcoI C'CATG_G

Cuts at:      0     279     808    1881 
   Size:        279     529    1073

  Fragments arranged by size: 

                1073     529     279
 
NdeI CA'TA_TG

Cuts at:      0     560    1314    1881 
   Size:        560     754     567

  Fragments arranged by size: 

                 754     567     560
 
NsiI A_TGCA'T

Cuts at:      0    1163    1881 
   Size:       1163     718
 
NspI r_CATG'y

Cuts at:      0     108     683     917    1246    1881 
   Size:        108     575     234     329     635

  Fragments arranged by size: 

                 635     575     329     234     108
 
PciI A'CATG_T

Cuts at:      0     679    1242    1881 
   Size:        679     563     639

  Fragments arranged by size: 

                 679     639     563
 
PfoI T'CCnGG_A

Cuts at:      0     461     644    1881 
   Size:        461     183    1237

  Fragments arranged by size: 

                1237     461     183
 
PpuMI rG'GwC_Cy

Cuts at:      0     390    1615    1881 
   Size:        390    1225     266

  Fragments arranged by size: 

                1225     390     266
 
SexAI A'CCwGG_T

Cuts at:      0     789    1881 
   Size:        789    1092
 
SfcI C'TryA_G

Cuts at:      0     452    1073    1562    1881 
   Size:        452     621     489     319

  Fragments arranged by size: 

                 621     489     452     319
 
SmlI C'TyrA_G

Cuts at:      0    1193    1881 
   Size:       1193     688
 
SspI AAT'ATT

Cuts at:      0    1536    1881 
   Size:       1536     345
 
StuI AGG'CCT

Cuts at:      0     458    1881 
   Size:        458    1423
 
StyI C'CwwG_G

Cuts at:      0     279     411     808    1881 
   Size:        279     132     397    1073

  Fragments arranged by size: 

                1073     397     279     132
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1423    1881 
   Size:       1423     458
 
XhoI C'TCGA_G

Cuts at:      0    1193    1881 
   Size:       1193     688
 
XmnI GAAnn'nnTTC

Cuts at:      0    1727    1881 
   Size:       1727     154
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AlfI    AlwNI     ApoI     AvaI    BamHI
     BanI     BbsI    BciVI     BclI    BfrBI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BsaHI    BseRI     BsgI     BsmI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    CspCI     DraI     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI    Hin4I   HincII
     HpaI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NsiI
     NspI     PciI     PfoI    PpuMI    SexAI     SfcI     SmlI     SspI
     StuI     StyI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BanII     BbeI    BbvCI     BcgI     BglI    BglII     BlpI
     BmtI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BsrGI   BssHII    BssSI
   BstAPI    BtgZI     BtsI     ClaI   DraIII     DrdI     EagI  EcoICRI
    EcoRI     FseI     FspI    FspAI    HaeII  HindIII     KasI     KpnI
     MfeI     MluI     NaeI     NarI   NgoMIV     NheI     NotI     NruI
     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com