Restriction map for pF1KB8635
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BalI        BamHI       BbrPI       BbsI        
BbvCI       BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaWI       BseAI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DdeI        DraI        DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MspA1I      
MunI        NcoI        NdeI        NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tsp509I     Tth111I     TthHB8I     XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcyI          369  GGCGCC               G[GA]^CG[CT]C
AflIII        104  ACGTGT               A^C[GA][CT]GT
AlwNI          81  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          183  GGGCCC               GGGCC^C
BanI          369  GGCGCC               G^G[CT][GA]CC
BbeI          369  GGCGCC               GGCGC^C
BcgI          376  GCACCGTCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI           52  CTAG                 C^TAG
BsaHI         369  GGCGCC               G[GA]^CG[CT]C
BsaI          532  GAGACC               GGTCTC(1/5)
BsiEI         395  CGATCG               CG[GA][CT]^CG
BsmAI         532  GAGAC                GTCTC(1/5)
BsrBI         650  CCGCTC               CCGCTC(-3/-3)
BsrI          251  ACTGG                ACTGG(1/-1)
BssHII        490  GCGCGC               G^CGCGC
BssSI         292  CACGAG               CACGAG(-5/-1)
BstF5I        708  GGATG                GGATG(2/0)
DraIII        100  CACGACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          355  CCACGG               C^C[GA][CT]GG
EcoNI          51  CCTAGACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI          708  GGATG                GGATG(9/13)
HaeII         369  GGCGCC               [GA]GCGC^[CT]
HgaI          680  GCGTC                GACGC(5/10)
Hin1I         369  GGCGCC               G[GA]^CG[CT]C
HinfI         390  GACTC                G^A[ACGT]TC
KasI          369  GGCGCC               G^GCGCC
MaeI           52  CTAG                 C^TAG
MaeII         104  ACGT                 A^CGT
MaeIII        469  GTGAC                ^GT[ACGT]AC
MslI          416  CACCTCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          369  GGCGCC               GG^CGCC
PflMI         206  CCATCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          390  GACTC                GAGTC(4/5)
PstI          282  CTGCAG               CTGCA^G
PvuI          395  CGATCG               CGAT^CG
SapI           69  GAAGAGC              GCTCTTC(1/4)
SfcI          282  CTGCAG               C^T[GA][CT]AG
SfoI          369  GGCGCC               GGC^GCC
SmlI          256  CTCAAG               C^T[CT][GA]AG
Tsp45I        469  GTGAC                ^GT[GC]AC
TspRI         153  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        206  CCATCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AluI           14  AGCT                 AG^CT
               95  AGCT                
AvaI          384  CTCGGG               C^[CT]CG[GA]G
              462  CTCGGG              
BanII         183  GGGCCC               G[GA]GC[CT]^C
              578  GAGCCC              
BmyI          183  GGGCCC               G[AGT]GC[ACT]^C
              578  GAGCCC              
BsoBI         384  CTCGGG               C^[CT]CG[GA]G
              462  CTCGGG              
Bsp1286I      183  GGGCCC               G[AGT]GC[ACT]^C
              578  GAGCCC              
BspMI          85  ACCTGC               ACCTGC(4/8)
              231  GCAGGT              
BsrFI         287  GCCGGC               [GA]^CCGG[CT]
              540  GCCGGC              
Cfr10I        287  GCCGGC               [GA]^CCGG[CT]
              540  GCCGGC              
DpnII         396  GATC                 ^GATC
              591  GATC                
EarI           40  CTCTTC               CTCTTC(1/4)
               69  GAAGAG              
HaeIII        184  GGCC                 GG^CC
              477  GGCC                
Ksp632I        40  CTCTTC               CTCTTC(1/4)
               69  GAAGAG              
MboI          396  GATC                 ^GATC
              591  GATC                
MboII          69  GAAGA                GAAGA(8/7)
               41  TCTTC               
NaeI          287  GCCGGC               GCC^GGC
              540  GCCGGC              
NdeII         396  GATC                 ^GATC
              591  GATC                
NgoMIV        287  GCCGGC               G^CCGGC
              540  GCCGGC              
Sau3AI        396  GATC                 ^GATC
              591  GATC                
SfaNI         709  GATGC                GCATC(5/9)
              278  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AccII       AciI        AvaII       BbvI        BcnI        
BpmI        BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmFI       BssKI       BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DraII       EcoO109I    
EcoRII      FauI        Fnu4HI      HapII       HhaI        
HinP1I      HpaII       HphI        ItaI        MnlI        
MspI        MvaI        MvnI        MwoI        NciI        
NlaIV       PpuMI       Sau96I      ScrFI       TseI        
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