Restriction map for pF1KB8635
 (Linear) (Six-base) MAPSORT of: KIBB8635.seq  Check: 4757  from: 1  to: 723



KIBB8635.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     222     723 
   Size:        222     501
 
AflIII A'CryG_T

Cuts at:      0     104     723 
   Size:        104     619
 
AleI CACnn'nnGTG

Cuts at:      0     420     723 
   Size:        420     303
 
AlwNI CAG_nnn'CTG

Cuts at:      0      86     723 
   Size:         86     637
 
ApaI G_GGCC'C

Cuts at:      0     187     723 
   Size:        187     536
 
AvaI C'yCGr_G

Cuts at:      0     384     462     723 
   Size:        384      78     261

  Fragments arranged by size: 

                 384     261      78
 
BanI G'GyrC_C

Cuts at:      0     369     723 
   Size:        369     354
 
BanII G_rGCy'C

Cuts at:      0     187     582     723 
   Size:        187     395     141

  Fragments arranged by size: 

                 395     187     141
 
BbeI G_GCGC'C

Cuts at:      0     373     723 
   Size:        373     350
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     365     399     723 
   Size:        365      34     324

  Fragments arranged by size: 

                 365     324      34
 
Bme1580I G_kGCm'C

Cuts at:      0     187     723 
   Size:        187     536
 
BmgBI CAC'GTC

Cuts at:      0     105     723 
   Size:        105     618
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     204     557     723 
   Size:        145      59     353     166

  Fragments arranged by size: 

                 353     166     145      59
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     723 
   Size:        241     482
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     526     723 
   Size:        526     197
 
BsaHI Gr'CG_yC

Cuts at:      0     370     723 
   Size:        370     353
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28      58     569     599     723 
   Size:         28      30     511      30     124

  Fragments arranged by size: 

                 511     124      30      30      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     307     622     644     723 
   Size:        307     315      22      79

  Fragments arranged by size: 

                 315     307      79      22
 
BseYI C'CCAG_C

Cuts at:      0      11      92     526     723 
   Size:         11      81     434     197

  Fragments arranged by size: 

                 434     197      81      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      19     121     250     258     359     723 
   Size:         19     102     129       8     101     364

  Fragments arranged by size: 

                 364     129     102     101      19       8
 
BsiEI CG_ry'CG

Cuts at:      0     398     723 
   Size:        398     325
 
Bsp1286I G_dGCh'C

Cuts at:      0     187     582     723 
   Size:        187     395     141

  Fragments arranged by size: 

                 395     187     141
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      94     222     723 
   Size:         94     128     501

  Fragments arranged by size: 

                 501     128      94
 
BsrBI CCG'CTC

Cuts at:      0     652     723 
   Size:        652      71
 
BsrFI r'CCGG_y

Cuts at:      0     287     540     723 
   Size:        287     253     183

  Fragments arranged by size: 

                 287     253     183
 
BssHII G'CGCG_C

Cuts at:      0     490     723 
   Size:        490     233
 
BssSI C'ACGA_G

Cuts at:      0     292     723 
   Size:        292     431
 
BtgI C'CryG_G

Cuts at:      0     355     723 
   Size:        355     368
 
DraIII CAC_nnn'GTG

Cuts at:      0     105     723 
   Size:        105     618
 
EarI CTCTTCn'nnn_

Cuts at:      0      46      64     723 
   Size:         46      18     659

  Fragments arranged by size: 

                 659      46      18
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     532     721     723 
   Size:        532     189       2

  Fragments arranged by size: 

                 532     189       2
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     204     557     723 
   Size:        145      59     353     166

  Fragments arranged by size: 

                 353     166     145      59
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      55     723 
   Size:         55     668
 
EcoO109I rG'GnC_Cy

Cuts at:      0      83     114     430     723 
   Size:         83      31     316     293

  Fragments arranged by size: 

                 316     293      83      31
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     189     282     723 
   Size:        189      93     441

  Fragments arranged by size: 

                 441     189      93
 
HaeII r_GCGC'y

Cuts at:      0     373     723 
   Size:        373     350
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     445     477     723 
   Size:        445      32     246

  Fragments arranged by size: 

                 445     246      32
 
KasI G'GCGC_C

Cuts at:      0     369     723 
   Size:        369     354
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     384     723 
   Size:        384     339
 
MslI CAynn'nnrTG

Cuts at:      0     420     723 
   Size:        420     303
 
NaeI GCC'GGC

Cuts at:      0     289     542     723 
   Size:        289     253     181

  Fragments arranged by size: 

                 289     253     181
 
NarI GG'CG_CC

Cuts at:      0     370     723 
   Size:        370     353
 
NgoMIV G'CCGG_C

Cuts at:      0     287     540     723 
   Size:        287     253     183

  Fragments arranged by size: 

                 287     253     183
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     212     723 
   Size:        212     511
 
PfoI T'CCnGG_A

Cuts at:      0     546     573     723 
   Size:        546      27     150

  Fragments arranged by size: 

                 546     150      27
 
PpuMI rG'GwC_Cy

Cuts at:      0      83     114     430     723 
   Size:         83      31     316     293

  Fragments arranged by size: 

                 316     293      83      31
 
PspOMI G'GGCC_C

Cuts at:      0     183     723 
   Size:        183     540
 
PstI C_TGCA'G

Cuts at:      0     286     723 
   Size:        286     437
 
PvuI CG_AT'CG

Cuts at:      0     398     723 
   Size:        398     325
 
SapI GCTCTTCn'nnn_

Cuts at:      0      64     723 
   Size:         64     659
 
SfcI C'TryA_G

Cuts at:      0     282     723 
   Size:        282     441
 
SfoI GGC'GCC

Cuts at:      0     371     723 
   Size:        371     352
 
SmlI C'TyrA_G

Cuts at:      0     256     723 
   Size:        256     467
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     423     723 
   Size:        423     300
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      28      60     130     162     569     601     723 
   Size:         28      32      70      32     407      32     122

  Fragments arranged by size: 

                 407     122      70      32      32      32      28
 

 Enzymes that do cut:

     AarI   AflIII     AleI    AlwNI     ApaI     AvaI     BanI    BanII
     BbeI     BcgI Bme1580I    BmgBI     BpmI    BpuEI     BsaI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI Bsp1286I    BspMI    BsrBI
    BsrFI   BssHII    BssSI     BtgI   DraIII     EarI     EciI  Eco57MI
    EcoNI EcoO109I     FalI    HaeII    Hin4I     KasI     MmeI     MslI
     NaeI     NarI   NgoMIV    PflMI     PfoI    PpuMI   PspOMI     PstI
     PvuI     SapI     SfcI     SfoI     SmlI    TaqII     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI    BbvCI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI     BmrI     BmtI     BplI   Bpu10I    BsaAI
    BsaBI    BsaWI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrDI    BsrGI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MscI   MspA1I     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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