Restriction map for
pF1KE0135
(Linear) (Six-base) MAPSORT of: KIEE0135.seq Check: 7415 from: 1 to: 1017
KIEE0135.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 591 696 1017
Size: 591 105 321
Fragments arranged by size:
591 321 105
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 682 934 1017
Size: 682 252 83
Fragments arranged by size:
682 252 83
AlwNI CAG_nnn'CTG
Cuts at: 0 614 678 968 1017
Size: 614 64 290 49
Fragments arranged by size:
614 290 64 49
AvaI C'yCGr_G
Cuts at: 0 691 1017
Size: 691 326
BanI G'GyrC_C
Cuts at: 0 16 629 1017
Size: 16 613 388
Fragments arranged by size:
613 388 16
BanII G_rGCy'C
Cuts at: 0 179 1017
Size: 179 838
BbeI G_GCGC'C
Cuts at: 0 20 1017
Size: 20 997
BbvCI CC'TCA_GC
Cuts at: 0 721 922 1017
Size: 721 201 95
Fragments arranged by size:
721 201 95
BclI T'GATC_A
Cuts at: 0 767 1017
Size: 767 250
BglII A'GATC_T
Cuts at: 0 347 1017
Size: 347 670
Bme1580I G_kGCm'C
Cuts at: 0 632 1017
Size: 632 385
BmrI ACTGGGnnnn_n'
Cuts at: 0 475 624 1017
Size: 475 149 393
Fragments arranged by size:
475 393 149
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 336 391 1017
Size: 322 14 55 626
Fragments arranged by size:
626 322 55 14
Bpu10I CC'TnA_GC
Cuts at: 0 721 922 1017
Size: 721 201 95
Fragments arranged by size:
721 201 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1017
Size: 163 854
BsaI GGTCTCn'nnnn_
Cuts at: 0 202 503 1017
Size: 202 301 514
Fragments arranged by size:
514 301 202
BsaBI GATnn'nnATC
Cuts at: 0 598 1017
Size: 598 419
BsaHI Gr'CG_yC
Cuts at: 0 17 878 1017
Size: 17 861 139
Fragments arranged by size:
861 139 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 388 409 418 436 439 532 535
Size: 124 264 21 9 18 3 93 3
Cuts at: 535 538 604 939 1017
Size: 3 66 335 78
Fragments arranged by size:
335 264 124 93 78 66 21 18
9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 968 1017
Size: 968 49
BsiEI CG_ry'CG
Cuts at: 0 36 1017
Size: 36 981
BsiHKAI G_wGCw'C
Cuts at: 0 179 1017
Size: 179 838
Bsp1286I G_dGCh'C
Cuts at: 0 179 632 1017
Size: 179 453 385
Fragments arranged by size:
453 385 179
BspHI T'CATG_A
Cuts at: 0 119 852 1017
Size: 119 733 165
Fragments arranged by size:
733 165 119
BsrBI CCG'CTC
Cuts at: 0 249 1017
Size: 249 768
BsrFI r'CCGG_y
Cuts at: 0 31 632 1017
Size: 31 601 385
Fragments arranged by size:
601 385 31
BsrGI T'GTAC_A
Cuts at: 0 749 1017
Size: 749 268
BssSI C'ACGA_G
Cuts at: 0 199 1017
Size: 199 818
BstAPI GCAn_nnn'nTGC
Cuts at: 0 678 1017
Size: 678 339
BstXI CCAn_nnnn'nTGG
Cuts at: 0 192 1017
Size: 192 825
BstYI r'GATC_y
Cuts at: 0 347 695 1017
Size: 347 348 322
Fragments arranged by size:
348 347 322
BtgI C'CryG_G
Cuts at: 0 191 1017
Size: 191 826
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 87 1017
Size: 87 930
DrdI GACnn_nn'nnGTC
Cuts at: 0 802 1017
Size: 802 215
EaeI y'GGCC_r
Cuts at: 0 33 263 410 1017
Size: 33 230 147 607
Fragments arranged by size:
607 230 147 33
EagI C'GGCC_G
Cuts at: 0 33 1017
Size: 33 984
EarI CTCTTCn'nnn_
Cuts at: 0 377 395 434 451 536 817 841 1017
Size: 377 18 39 17 85 281 24 176
Fragments arranged by size:
377 281 176 85 39 24 18 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 651 1017
Size: 651 366
EcoICRI GAG'CTC
Cuts at: 0 177 1017
Size: 177 840
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 336 391 591 696 1017
Size: 322 14 55 200 105 321
Fragments arranged by size:
322 321 200 105 55 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 373 682 1017
Size: 373 309 335
Fragments arranged by size:
373 335 309
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 780 969 1017
Size: 780 189 48
Fragments arranged by size:
780 189 48
FseI GG_CCGG'CC
Cuts at: 0 35 1017
Size: 35 982
FspI TGC'GCA
Cuts at: 0 152 1017
Size: 152 865
HaeII r_GCGC'y
Cuts at: 0 20 691 1017
Size: 20 671 326
Fragments arranged by size:
671 326 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 709 741 759 791 1017
Size: 166 32 511 32 18 32 226
Fragments arranged by size:
511 226 166 32 32 32 18
HincII GTy'rAC
Cuts at: 0 486 1017
Size: 486 531
HindIII A'AGCT_T
Cuts at: 0 307 492 1017
Size: 307 185 525
Fragments arranged by size:
525 307 185
KasI G'GCGC_C
Cuts at: 0 16 1017
Size: 16 1001
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 1017
Size: 152 865
MscI TGG'CCA
Cuts at: 0 265 1017
Size: 265 752
MslI CAynn'nnrTG
Cuts at: 0 857 1017
Size: 857 160
MspA1I CmG'CkG
Cuts at: 0 261 968 1017
Size: 261 707 49
Fragments arranged by size:
707 261 49
NaeI GCC'GGC
Cuts at: 0 33 1017
Size: 33 984
NarI GG'CG_CC
Cuts at: 0 17 1017
Size: 17 1000
NgoMIV G'CCGG_C
Cuts at: 0 31 1017
Size: 31 986
PflMI CCAn_nnn'nTGG
Cuts at: 0 968 1017
Size: 968 49
PstI C_TGCA'G
Cuts at: 0 293 683 1017
Size: 293 390 334
Fragments arranged by size:
390 334 293
PvuII CAG'CTG
Cuts at: 0 261 1017
Size: 261 756
SacI G_AGCT'C
Cuts at: 0 179 1017
Size: 179 838
SapI GCTCTTCn'nnn_
Cuts at: 0 536 1017
Size: 536 481
SfcI C'TryA_G
Cuts at: 0 289 679 1017
Size: 289 390 338
Fragments arranged by size:
390 338 289
SfoI GGC'GCC
Cuts at: 0 18 1017
Size: 18 999
SgrAI Cr'CCGG_yG
Cuts at: 0 632 1017
Size: 632 385
SmlI C'TyrA_G
Cuts at: 0 178 691 1017
Size: 178 513 326
Fragments arranged by size:
513 326 178
StyI C'CwwG_G
Cuts at: 0 281 809 1017
Size: 281 528 208
Fragments arranged by size:
528 281 208
TatI w'GTAC_w
Cuts at: 0 749 1004 1017
Size: 749 255 13
Fragments arranged by size:
749 255 13
XhoI C'TCGA_G
Cuts at: 0 691 1017
Size: 691 326
XmnI GAAnn'nnTTC
Cuts at: 0 709 1017
Size: 709 308
Enzymes that do cut:
AcuI AlfI AlwNI AvaI BanI BanII BbeI BbvCI
BclI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI
BsrBI BsrFI BsrGI BssSI BstAPI BstXI BstYI BtgI
BtgZI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI FalI FseI FspI HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
PflMI PstI PvuII SacI SapI SfcI SfoI SgrAI
SmlI StyI TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI
BfrBI BglI BlpI BmgBI BmtI BplI BsaAI BsaWI
BsaXI BsgI BsiWI BsmI BsmBI BspEI BspMI BsrDI
BssHII BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII EcoO109I EcoRI EcoRV FspAI HpaI KpnI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XmaI ZraI