Restriction map for pF1KE0442
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AvaI        AvaII       AviII       AvrII       BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BpmI        BpuAI       BsaAI       
BsaBI       BsaHI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsoBI       Bsp1407I    
BspHI       BspLU11I    BsrBI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Cfr10I      CpoI        DraI        DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HpaI        
KasI        KpnI        KspI        MaeII       MaeIII      
MamI        MfeI        MluI        MseI        MspA1I      
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        SspBI       
SspI        StuI        StyI        SwaI        Tru9I       
Tsp45I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccIII        708  TCCGGA               T^CCGGA
AcsI          398  AAATTT               [GA]^AATT[CT]
AfaI          714  GTAC                 GT^AC
AlwI          183  GATCC                GGATC(4/5)
AlwNI         373  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          903  CCTCAGG              CC^T[ACGT]AGG
ApoI          398  AAATTT               [GA]^AATT[CT]
Asp700I       456  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          790  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          545  TGGCCA               TGG^CCA
BbvCI         666  CCTCAGC              CCTCAGC(-5/-2)
BcnI          444  CCCGG                CC^[GC]GG
BlpI           73  GCTCAGC              GC^T[ACGT]AGC
BmyI          246  GTGCCC               G[AGT]GC[ACT]^C
Bpu1102I       73  GCTCAGC              GC^T[ACGT]AGC
BsaI          739  GAGACC               GGTCTC(1/5)
BsaWI         708  TCCGGA               [AT]^CCGG[AT]
BseAI         708  TCCGGA               T^CCGGA
BsmFI         558  GTCCC                GGGAC(10/14)
BsmI          237  GAATGC               GAATGC(1/-1)
Bsp1286I      246  GTGCCC               G[AGT]GC[ACT]^C
BspDI         832  ATCGAT               AT^CGAT
BspEI         708  TCCGGA               T^CCGGA
BstYI         182  AGATCC               [GA]^GATC[CT]
Bsu36I        903  CCTCAGG              CC^T[ACGT]AGG
CelII          73  GCTCAGC              GC^T[ACGT]AGC
ClaI          832  ATCGAT               AT^CGAT
DraIII        799  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI          545  TGGCCA               [CT]^GGCC[GA]
Eco81I        903  CCTCAGG              CC^T[ACGT]AGG
HaeII         115  AGCGCC               [GA]GCGC^[CT]
HindIII       230  AAGCTT               A^AGCTT
HphI          762  GGTGA                GGTGA(8/7)
MflI          182  AGATCC               [GA]^GATC[CT]
MluNI         545  TGGCCA               TGG^CCA
MroI          708  TCCGGA               T^CCGGA
MscI          545  TGGCCA               TGG^CCA
MwoI          232  GCTTGGAATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          444  CCCGG                CC^[GC]GG
NspI          506  ACATGC               [GA]CATG^[CT]
PflFI         790  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          773  GAGTC                GAGTC(4/5)
PshAI         678  GACAAGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          154  CTGCAG               CTGCA^G
RsaI          714  GTAC                 GT^AC
SapI          304  GCTCTTC              GCTCTTC(1/4)
SbfI          153  CCTGCAGG             CCTGCA^GG
Sse8387I      153  CCTGCAGG             CCTGCA^GG
TspRI         879  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       790  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         182  AGATCC               [GA]^GATC[CT]
XmnI          456  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AciI          495  CCGC                 CCGC(-3/-1)
              241  GCGG                
BbvI          845  GCTGC                GCAGC(8/12)
              891  GCTGC               
BfaI          295  CTAG                 C^TAG
              514  CTAG                
BsaJI          26  CCAGGG               C^C[ACGT][ACGT]GG
              377  CCCTGG              
BsmAI         739  GAGAC                GTCTC(1/5)
              622  GTCTC               
BspMI           8  ACCTGC               ACCTGC(4/8)
              152  ACCTGC              
BsrDI         602  GCAATG               GCAATG(2/0)
              958  GCAATG              
CfoI          116  GCGC                 GCG^C
              170  GCGC                
DraII         374  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              869  AGGCCCC             
EcoNI          79  CCTACAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              378  CCTGGCAAAGG         
EcoO109I      374  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              869  AGGCCCC             
Fnu4HI        845  GCTGC                GC^[ACGT]GC
              891  GCTGC               
HapII         445  CCGG                 C^CGG
              709  CCGG                
HhaI          116  GCGC                 GCG^C
              170  GCGC                
HinP1I        116  GCGC                 G^CGC
              170  GCGC                
HpaII         445  CCGG                 C^CGG
              709  CCGG                
ItaI          845  GCTGC                GC^[ACGT]GC
              891  GCTGC               
MaeI          295  CTAG                 C^TAG
              514  CTAG                
MslI          579  CACGCCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              756  CATGAGGGTG          
MspI          445  CCGG                 C^CGG
              709  CCGG                
PflMI         354  CCAACACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              798  CCACCCTGTGG         
TseI          845  GCTGC                G^C[AT]GC
              891  GCTGC               
Van91I        354  CCAACACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              798  CCACCCTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AluI        BsiYI       BslI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
EarI        EcoRII      FokI        HaeIII      HinfI       
Ksp632I     MboI        MboII       MnlI        MvaI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TfiI        
Tsp509I     TthHB8I     
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