Restriction map for pF1KE0442
 (Linear) (Six-base) MAPSORT of: KIEE0442.seq  Check: 1054  from: 1  to: 969



KIEE0442.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     292     447     451     949     969 
   Size:        109     183     155       4     498      20

  Fragments arranged by size: 

                 498     183     155     109      20       4
 
AlwNI CAG_nnn'CTG

Cuts at:      0     378     969 
   Size:        378     591
 
ApoI r'AATT_y

Cuts at:      0     398     969 
   Size:        398     571
 
BbvCI CC'TCA_GC

Cuts at:      0     667     969 
   Size:        667     302
 
BlpI GC'TnA_GC

Cuts at:      0      74     969 
   Size:         74     895
 
Bme1580I G_kGCm'C

Cuts at:      0     250     969 
   Size:        250     719
 
Bpu10I CC'TnA_GC

Cuts at:      0     598     667     969 
   Size:        598      69     302

  Fragments arranged by size: 

                 598     302      69
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     733     969 
   Size:        733     236
 
BsaWI w'CCGG_w

Cuts at:      0     708     969 
   Size:        708     261
 
BsmI GAATG_Cn'

Cuts at:      0     243     969 
   Size:        243     726
 
Bsp1286I G_dGCh'C

Cuts at:      0     250     969 
   Size:        250     719
 
BspEI T'CCGG_A

Cuts at:      0     708     969 
   Size:        708     261
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      17     161     969 
   Size:         17     144     808

  Fragments arranged by size: 

                 808     144      17
 
BsrDI GCAATG_nn'

Cuts at:      0     609     965     969 
   Size:        609     356       4

  Fragments arranged by size: 

                 609     356       4
 
BstYI r'GATC_y

Cuts at:      0     182     969 
   Size:        182     787
 
Bsu36I CC'TnA_GG

Cuts at:      0     904     969 
   Size:        904      65
 
ClaI AT'CG_AT

Cuts at:      0     833     969 
   Size:        833     136
 
DraIII CAC_nnn'GTG

Cuts at:      0     804     969 
   Size:        804     165
 
EaeI y'GGCC_r

Cuts at:      0     545     969 
   Size:        545     424
 
EarI CTCTTCn'nnn_

Cuts at:      0     311     568     731     969 
   Size:        311     257     163     238

  Fragments arranged by size: 

                 311     257     238     163
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     292     447     451     949     969 
   Size:        109     183     155       4     498      20

  Fragments arranged by size: 

                 498     183     155     109      20       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      83     382     969 
   Size:         83     299     587

  Fragments arranged by size: 

                 587     299      83
 
EcoO109I rG'GnC_Cy

Cuts at:      0     375     870     969 
   Size:        375     495      99

  Fragments arranged by size: 

                 495     375      99
 
HaeII r_GCGC'y

Cuts at:      0     119     969 
   Size:        119     850
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     534     566     764     796     969 
   Size:        534      32     198      32     173

  Fragments arranged by size: 

                 534     198     173      32      32
 
HindIII A'AGCT_T

Cuts at:      0     230     969 
   Size:        230     739
 
MscI TGG'CCA

Cuts at:      0     547     969 
   Size:        547     422
 
MslI CAynn'nnrTG

Cuts at:      0     583     760     969 
   Size:        583     177     209

  Fragments arranged by size: 

                 583     209     177
 
NspI r_CATG'y

Cuts at:      0     510     969 
   Size:        510     459
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     360     804     969 
   Size:        360     444     165

  Fragments arranged by size: 

                 444     360     165
 
PfoI T'CCnGG_A

Cuts at:      0      56     969 
   Size:         56     913
 
PshAI GACnn'nnGTC

Cuts at:      0     682     969 
   Size:        682     287
 
PstI C_TGCA'G

Cuts at:      0     158     969 
   Size:        158     811
 
SapI GCTCTTCn'nnn_

Cuts at:      0     311     969 
   Size:        311     658
 
SbfI CC_TGCA'GG

Cuts at:      0     158     969 
   Size:        158     811
 
SfcI C'TryA_G

Cuts at:      0      80     154     471     969 
   Size:         80      74     317     498

  Fragments arranged by size: 

                 498     317      80      74
 
TatI w'GTAC_w

Cuts at:      0     713     969 
   Size:        713     256
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      38      70     969 
   Size:         38      32     899

  Fragments arranged by size: 

                 899      38      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     793     969 
   Size:        793     176
 
XmnI GAAnn'nnTTC

Cuts at:      0     460     969 
   Size:        460     509
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    BbvCI     BlpI Bme1580I   Bpu10I     BsaI
    BsaWI     BsmI Bsp1286I    BspEI    BspMI    BsrDI    BstYI   Bsu36I
     ClaI   DraIII     EaeI     EarI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I  HindIII     MscI     MslI     NspI    PflMI     PfoI    PshAI
     PstI     SapI     SbfI     SfcI     TatI     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI    CspCI
     DraI     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     XbaI
     XcmI     XhoI     XmaI     ZraI
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