Restriction map for pF1KE0152
 (Circular) (Six-base) MAPSORT of: pF1KE0152.seq  Check: 6803  from: 1  to: 3506



pF1KE0152  3506 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AccI GT'mk_AC

Cuts at:    544     544 
   Size:       3506
 
Acc65I G'GTAC_C

Cuts at:    522     522 
   Size:       3506
 
AclI AA'CG_TT

Cuts at:   3346    3346 
   Size:       3506
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1331    1763    2510    2778    1331 
   Size:        432     747     268    2059

  Fragments arranged by size: 

                2059     747     432     268
 
AflIII A'CryG_T

Cuts at:    858    1962    2939     858 
   Size:       1104     977    1425

  Fragments arranged by size: 

                1425    1104     977
 
AgeI A'CCGG_T

Cuts at:   1894    1894 
   Size:       3506
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    443     443 
   Size:       3506
 
AlwNI CAG_nnn'CTG

Cuts at:    165     811    2378     165 
   Size:        646    1567    1293

  Fragments arranged by size: 

                1567    1293     646
 
ApaLI G'TGCA_C

Cuts at:   2276    2276 
   Size:       3506
 
ApoI r'AATT_y

Cuts at:    510    2859    3115     510 
   Size:       2349     256     901

  Fragments arranged by size: 

                2349     901     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3506
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3506
 
AvaI C'yCGr_G

Cuts at:    433     526     433 
   Size:         93    3413
 
BamHI G'GATC_C

Cuts at:    531     531 
   Size:       3506
 
BanI G'GyrC_C

Cuts at:    522     936    1184    1219     522 
   Size:        414     248      35    2809

  Fragments arranged by size: 

                2809     414     248      35
 
BanII G_rGCy'C

Cuts at:    520    1550     520 
   Size:       1030    2476
 
BbeI G_GCGC'C

Cuts at:    940    1188     940 
   Size:        248    3258
 
BciVI GTATCCnnnnn_n'

Cuts at:   1404    2165    3506    1404 
   Size:        761    1341    1404

  Fragments arranged by size: 

                1404    1341     761
 
BclI T'GATC_A

Cuts at:    167    1026     167 
   Size:        859    2647
 
BglII A'GATC_T

Cuts at:    201    1021    3058     201 
   Size:        820    2037     649

  Fragments arranged by size: 

                2037     820     649
 
BlpI GC'TnA_GC

Cuts at:    618     618 
   Size:       3506
 
Bme1580I G_kGCm'C

Cuts at:   1131    1224    2280    1131 
   Size:         93    1056    2357

  Fragments arranged by size: 

                2357    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    718     799    1134    3278     718 
   Size:         81     335    2144     946

  Fragments arranged by size: 

                2144     946     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1873    1873 
   Size:       3506
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    415     682    1561    2053    2351     415 
   Size:        267     879     492     298    1570

  Fragments arranged by size: 

                1570     879     492     298     267
 
BsaI GGTCTCn'nnnn_

Cuts at:    287    3210     287 
   Size:       2923     583
 
BsaAI yAC'GTr

Cuts at:    859     859 
   Size:       3506
 
BsaBI GATnn'nnATC

Cuts at:      6     137    1020    3057       6 
   Size:        131     883    2037     455

  Fragments arranged by size: 

                2037     883     455     131
 
BsaHI Gr'CG_yC

Cuts at:    937    1185    1887    3383     937 
   Size:        248     702    1496    1060

  Fragments arranged by size: 

                1496    1060     702     248
 
BsaWI w'CCGG_w

Cuts at:    695    1216    1894    2168    2315    2726     695 
   Size:        521     678     274     147     411    1475

  Fragments arranged by size: 

                1475     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    236     236 
   Size:       3506
 
BseYI C'CCAG_C

Cuts at:    931    1689    2266    2762     931 
   Size:        758     577     496    1675

  Fragments arranged by size: 

                1675     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1048    1094    2302     104 
   Size:        944      46    1208    1308

  Fragments arranged by size: 

                1308    1208     944      46
 
BsiHKAI G_wGCw'C

Cuts at:    520    1298    1488    2280     520 
   Size:        778     190     792    1746

  Fragments arranged by size: 

                1746     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    520    1131    1224    1298    1488    1550    2280     520 
   Size:        611      93      74     190      62     730    1746

  Fragments arranged by size: 

                1746     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2726    2726 
   Size:       3506
 
BspHI T'CATG_A

Cuts at:   2682    2682 
   Size:       3506
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    556     770    1072    1453     556 
   Size:        214     302     381    2609

  Fragments arranged by size: 

                2609     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1798    1852    3336    3501      50 
   Size:       1748      54    1484     165      55

  Fragments arranged by size: 

                1748    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1418    1418 
   Size:       3506
 
BsrFI r'CCGG_y

Cuts at:   1504    1685    1894    1504 
   Size:        181     209    3116

  Fragments arranged by size: 

                3116     209     181
 
BssSI C'ACGA_G

Cuts at:   1777    2135    1777 
   Size:        358    3148
 
BstAPI GCAn_nnn'nTGC

Cuts at:    408     883     408 
   Size:        475    3031
 
BstBI TT'CG_AA

Cuts at:   1867    1867 
   Size:       3506
 
BstYI r'GATC_y

Cuts at:      1     201     531     998    1021    1356    1602    2614    2700 
   Size:        200     330     467      23     335     246    1012      86

Cuts at:   2700    2712    3058       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     330     246     200
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3506
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1456    1637    1800    1456 
   Size:        181     163    3162

  Fragments arranged by size: 

                3162     181     163
 
BtsI GCAGTG_nn'

Cuts at:    749     749 
   Size:       3506
 
DraI TTT'AAA

Cuts at:    505     505 
   Size:       3506
 
DrdI GACnn_nn'nnGTC

Cuts at:   1212    2070    1212 
   Size:        858    2648
 
EaeI y'GGCC_r

Cuts at:    110    1091    1265    1656    1683    3028     110 
   Size:        981     174     391      27    1345     588

  Fragments arranged by size: 

                1345     981     588     391     174      27
 
EagI C'GGCC_G

Cuts at:   1091    1091 
   Size:       3506
 
EarI CTCTTCn'nnn_

Cuts at:    410    1529    1739     410 
   Size:       1119     210    2177

  Fragments arranged by size: 

                2177    1119     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1714    2024    2170    3316    1714 
   Size:        310     146    1146    1904

  Fragments arranged by size: 

                1904    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    518     518 
   Size:       3506
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1331    1763    2510    2778    1331 
   Size:        432     747     268    2059

  Fragments arranged by size: 

                2059     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    645     645 
   Size:       3506
 
EcoRI G'AATT_C

Cuts at:    510     510 
   Size:       3506
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1525    1525 
   Size:       3506
 
FspI TGC'GCA

Cuts at:   1287    1287 
   Size:       3506
 
HaeII r_GCGC'y

Cuts at:    940    1188    2210    3189     940 
   Size:        248    1022     979    1257

  Fragments arranged by size: 

                1257    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1348    1378    1380    1410    1348 
   Size:         30       2      30    3444

  Fragments arranged by size: 

                3444      30      30       2
 
HincII GTy'rAC

Cuts at:    545     545 
   Size:       3506
 
KasI G'GCGC_C

Cuts at:    936    1184     936 
   Size:        248    3258
 
KpnI G_GTAC'C

Cuts at:    526     526 
   Size:       3506
 
MluI A'CGCG_T

Cuts at:   2939    2939 
   Size:       3506
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    359    2177    2361     359 
   Size:       1818     184    1504

  Fragments arranged by size: 

                1818    1504     184
 
MscI TGG'CCA

Cuts at:   1267    1267 
   Size:       3506
 
MslI CAynn'nnrTG

Cuts at:   1495    1622    1495 
   Size:        127    3379
 
MspA1I CmG'CkG

Cuts at:    617     931    1291    2304    2549     617 
   Size:        314     360    1013     245    1574

  Fragments arranged by size: 

                1574    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1687    1687 
   Size:       3506
 
NarI GG'CG_CC

Cuts at:    937    1185     937 
   Size:        248    3258
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3506
 
NgoMIV G'CCGG_C

Cuts at:   1685    1685 
   Size:       3506
 
NspI r_CATG'y

Cuts at:    559    1059    1966     559 
   Size:        500     907    2099

  Fragments arranged by size: 

                2099     907     500
 
PciI A'CATG_T

Cuts at:   1962    1962 
   Size:       3506
 
PfoI T'CCnGG_A

Cuts at:    187     187 
   Size:       3506
 
PmeI GTTT'AAAC

Cuts at:    505     505 
   Size:       3506
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2645    2677    2645 
   Size:         32    3474
 
PstI C_TGCA'G

Cuts at:    553    1238     553 
   Size:        685    2821
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3506
 
PvuII CAG'CTG

Cuts at:    931    1291     931 
   Size:        360    3146
 
RsrII CG'GwC_CG

Cuts at:   1701    1701 
   Size:       3506
 
SacI G_AGCT'C

Cuts at:    520     520 
   Size:       3506
 
SalI G'TCGA_C

Cuts at:    543     543 
   Size:       3506
 
SapI GCTCTTCn'nnn_

Cuts at:    410    1529    1739     410 
   Size:       1119     210    2177

  Fragments arranged by size: 

                2177    1119     210
 
SbfI CC_TGCA'GG

Cuts at:    553     553 
   Size:       3506
 
ScaI AGT'ACT

Cuts at:   3023    3023 
   Size:       3506
 
SfcI C'TryA_G

Cuts at:     33     549    1234    2227    2418      33 
   Size:        516     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     516     191
 
SfoI GGC'GCC

Cuts at:    938    1186     938 
   Size:        248    3258
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3506
 
SmaI CCC'GGG

Cuts at:    528     528 
   Size:       3506
 
SmlI C'TyrA_G

Cuts at:    394     661    1576    2068    2330     394 
   Size:        267     915     492     262    1570

  Fragments arranged by size: 

                1570     915     492     267     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3506
 
SphI G_CATG'C

Cuts at:    559    1059     559 
   Size:        500    3006
 
StuI AGG'CCT

Cuts at:    156     156 
   Size:       3506
 
StyI C'CwwG_G

Cuts at:    107     640     994     107 
   Size:        533     354    2619

  Fragments arranged by size: 

                2619     533     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1089    1222    1681    1890    1089 
   Size:        133     459     209    2705

  Fragments arranged by size: 

                2705     459     209     133
 
TatI w'GTAC_w

Cuts at:   3021    3021 
   Size:       3506
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3023    3055    3023 
   Size:         32    3474
 
Tth111I GACn'n_nGTC

Cuts at:   1303    1303 
   Size:       3506
 
XbaI T'CTAG_A

Cuts at:    537     537 
   Size:       3506
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1001    1001 
   Size:       3506
 
XmaI C'CCGG_G

Cuts at:    526     526 
   Size:       3506
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PciI     PfoI     PmeI     PpiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI     BpmI   Bpu10I    BsaXI     BsgI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     SwaI     XhoI     XmnI     ZraI
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