Restriction map for pF1KB8348
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AhdI        Alw44I      AocI        ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvaI        AviII       AvrII       BbrPI       
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BsaAI       BsaI        BseAI       BsiEI       
BsiWI       BsmBI       BsoBI       BspEI       BspLU11I    
BspMI       BsrDI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstZ17I     Bsu36I      CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      Eco81I      EcoNI       EcoRV       
FbaI        FseI        FspI        HindIII     HpaI        
KpnI        KspI        MfeI        MroI        MunI        
NaeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspI        StuI        SwaI        XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   67 enzymes) #
###################################

AatII         460  GACGTC               GACGT^C
AcsI         1359  GAATTC               [GA]^AATT[CT]
AflIII        147  ACGCGT               A^C[GA][CT]GT
AgeI          781  ACCGGT               A^CCGGT
AlwNI         421  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1151  AGCGCT               AGC^GCT
ApoI         1359  GAATTC               [GA]^AATT[CT]
AspHI         955  GAGCTC               G[AT]GC[AT]^C
AspI           99  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1026  GGATCC               G^GATCC
BbeI         1293  GGCGCC               GGCGC^C
BbsI          850  GAAGAC               GAAGAC(2/6)
BbvCI         154  CCTCAGC              CCTCAGC(-5/-2)
BcgI          368  GCAGAGTTATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         1092  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1092  GCTCAGC              GC^T[ACGT]AGC
BpuAI         850  GAAGAC               GAAGAC(2/6)
BsaBI         871  GATCCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         781  ACCGGT               [AT]^CCGG[AT]
BsgI          677  GTGCAG               GTGCAG(16/14)
BsiHKAI       955  GAGCTC               G[AT]GC[AT]^C
BsmFI         151  GTCCC                GGGAC(10/14)
BsmI          250  GAATGC               GAATGC(1/-1)
Bsp1407I     1223  TGTACA               T^GTACA
BspDI         487  ATCGAT               AT^CGAT
BspHI        1130  TCATGA               T^CATGA
BsrBI        1057  GAGCGG               CCGCTC(-3/-3)
BsrFI         781  ACCGGT               [GA]^CCGG[CT]
BsrGI        1223  TGTACA               T^GTACA
BstEII        189  GGTCACC              G^GT[ACGT]ACC
BstPI         189  GGTCACC              G^GT[ACGT]ACC
CelII        1092  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        781  ACCGGT               [GA]^CCGG[CT]
ClaI          487  ATCGAT               AT^CGAT
DraII        1190  AGGCCCT              [GA]G^G[ACGT]CC[CT]
Eco47III     1151  AGCGCT               AGC^GCT
EcoO109I     1190  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       189  GGTCACC              G^GT[ACGT]ACC
EcoRI        1359  GAATTC               G^AATTC
EcoT22I       655  ATGCAT               ATGCA^T
FauI          269  GCGGG                CCCGC(4/6)
HincII        463  GTCAAC               GT[CT]^[GA]AC
HindII        463  GTCAAC               GT[CT]^[GA]AC
KasI         1293  GGCGCC               G^GCGCC
MaeII         461  ACGT                 A^CGT
MamI          871  GATCCTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI          147  ACGCGT               A^CGCGT
MslI          195  CATCAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        981  CAGCTG               C[AC]G^C[GT]G
NarI         1293  GGCGCC               GG^CGCC
NcoI         1143  CCATGG               C^CATGG
NsiI          655  ATGCAT               ATGCA^T
PflFI          99  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         916  CCAGGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         781  ACCGGT               A^CCGGT
PvuII         981  CAGCTG               CAG^CTG
RcaI         1130  TCATGA               T^CATGA
SacI          955  GAGCTC               GAGCT^C
SapI         1062  GAAGAGC              GCTCTTC(1/4)
SfaNI         986  GCATC                GCATC(5/9)
SfoI         1293  GGCGCC               GGC^GCC
SmlI          231  CTCAAG               C^T[CT][GA]AG
SphI          657  GCATGC               GCATG^C
SspBI        1223  TGTACA               T^GTACA
TfiI          882  GATTC                G^A[AT]TC
Tth111I        99  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        916  CCAGGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcyI          460  GACGTC               G[GA]^CG[CT]C
             1293  GGCGCC              
BanI         1000  GGCACC               G^G[CT][GA]CC
             1293  GGCGCC              
BcnI         1043  CCCGG                CC^[GC]GG
             1209  CCCGG               
BsaHI         460  GACGTC               G[GA]^CG[CT]C
             1293  GGCGCC              
BsmAI         589  GAGAC                GTCTC(1/5)
              171  GTCTC               
BstF5I        558  CATCC                GGATG(2/0)
              302  GGATG               
BstXI         194  CCATCAAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              219  CCAGGTGAATGG        
EarI          596  CTCTTC               CTCTTC(1/4)
             1062  GAAGAG              
FokI          558  CATCC                GGATG(9/13)
              302  GGATG               
Hin1I         460  GACGTC               G[GA]^CG[CT]C
             1293  GGCGCC              
Ksp632I       596  CTCTTC               CTCTTC(1/4)
             1062  GAAGAG              
MseI           14  TTAA                 T^TAA
              652  TTAA                
MwoI          159  GCAGGATTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              417  GCGGCAGTGGC         
NciI         1043  CCCGG                CC^[GC]GG
             1209  CCCGG               
NdeI          752  CATATG               CA^TATG
             1408  CATATG              
NspI          335  ACATGC               [GA]CATG^[CT]
              657  GCATGC              
PleI          591  GACTC                GAGTC(4/5)
             1213  GAGTC               
PshAI         853  GACCAGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1134  GACCAAGGTC          
Tru9I          14  TTAA                 T^TAA
              652  TTAA                
Tsp509I       729  AATT                 ^AATT
             1360  AATT                
XcmI          480  CCAGAACATCGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1345  CCAAACCCATACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaII       BalI        BanII       BbvI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EaeI        EcoRII      
EcoT14I     Fnu4HI      HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MspI        MvaI        MvnI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XhoII       
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