Restriction map for pF1KB8348
 (Linear) (Six-base) MAPSORT of: KIBB8348.seq  Check: 2951  from: 1  to: 1437



KIBB8348.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

AatII G_ACGT'C

Cuts at:      0     464    1437 
   Size:        464     973
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     596     686     734     869    1437 
   Size:        596      90      48     135     568

  Fragments arranged by size: 

                 596     568     135      90      48
 
AfeI AGC'GCT

Cuts at:      0    1153    1437 
   Size:       1153     284
 
AflIII A'CryG_T

Cuts at:      0     147    1437 
   Size:        147    1290
 
AgeI A'CCGG_T

Cuts at:      0     781    1437 
   Size:        781     656
 
AlwNI CAG_nnn'CTG

Cuts at:      0     426    1437 
   Size:        426    1011
 
ApoI r'AATT_y

Cuts at:      0    1359    1437 
   Size:       1359      78
 
BamHI G'GATC_C

Cuts at:      0    1026    1437 
   Size:       1026     411
 
BanI G'GyrC_C

Cuts at:      0    1000    1293    1437 
   Size:       1000     293     144

  Fragments arranged by size: 

                1000     293     144
 
BanII G_rGCy'C

Cuts at:      0     145     761     959    1258    1437 
   Size:        145     616     198     299     179

  Fragments arranged by size: 

                 616     299     198     179     145
 
BbeI G_GCGC'C

Cuts at:      0    1297    1437 
   Size:       1297     140
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     857    1437 
   Size:        857     580
 
BbvCI CC'TCA_GC

Cuts at:      0     155    1437 
   Size:        155    1282
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     357     391    1437 
   Size:        357      34    1046

  Fragments arranged by size: 

                1046     357      34
 
BfrBI ATG'CAT

Cuts at:      0     657    1437 
   Size:        657     780
 
BlpI GC'TnA_GC

Cuts at:      0    1093    1437 
   Size:       1093     344
 
Bme1580I G_kGCm'C

Cuts at:      0     842    1429    1437 
   Size:        842     587       8

  Fragments arranged by size: 

                 842     587       8
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     798    1379    1437 
   Size:        798     581      58

  Fragments arranged by size: 

                 798     581      58
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      96     448     963     973    1180    1289    1437 
   Size:         96     352     515      10     207     109     148

  Fragments arranged by size: 

                 515     352     207     148     109      96      10
 
Bpu10I CC'TnA_GC

Cuts at:      0     155    1437 
   Size:        155    1282
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     216    1437 
   Size:        216    1221
 
BsaBI GATnn'nnATC

Cuts at:      0     875    1437 
   Size:        875     562
 
BsaHI Gr'CG_yC

Cuts at:      0     461    1294    1437 
   Size:        461     833     143

  Fragments arranged by size: 

                 833     461     143
 
BsaWI w'CCGG_w

Cuts at:      0     781    1437 
   Size:        781     656
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     946     976    1240    1270    1437 
   Size:        946      30     264      30     167

  Fragments arranged by size: 

                 946     264     167      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      99     340     584    1437 
   Size:         99     241     244     853

  Fragments arranged by size: 

                 853     244     241      99
 
BseYI C'CCAG_C

Cuts at:      0     180     541    1049    1437 
   Size:        180     361     508     388

  Fragments arranged by size: 

                 508     388     361     180
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     698    1437 
   Size:        698     739
 
BsiHKAI G_wGCw'C

Cuts at:      0     959    1437 
   Size:        959     478
 
BsmI GAATG_Cn'

Cuts at:      0     256    1437 
   Size:        256    1181
 
Bsp1286I G_dGCh'C

Cuts at:      0     145     761     842     959    1258    1429    1437 
   Size:        145     616      81     117     299     171       8

  Fragments arranged by size: 

                 616     299     171     145     117      81       8
 
BspHI T'CATG_A

Cuts at:      0    1130    1437 
   Size:       1130     307
 
BsrBI CCG'CTC

Cuts at:      0    1059    1437 
   Size:       1059     378
 
BsrFI r'CCGG_y

Cuts at:      0     781    1437 
   Size:        781     656
 
BsrGI T'GTAC_A

Cuts at:      0    1223    1437 
   Size:       1223     214
 
BstEII G'GTnAC_C

Cuts at:      0     189    1437 
   Size:        189    1248
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     201     226    1437 
   Size:        201      25    1211

  Fragments arranged by size: 

                1211     201      25
 
BstYI r'GATC_y

Cuts at:      0     387     870     929    1026    1437 
   Size:        387     483      59      97     411

  Fragments arranged by size: 

                 483     411     387      97      59
 
BtgI C'CryG_G

Cuts at:      0     646    1143    1176    1437 
   Size:        646     497      33     261

  Fragments arranged by size: 

                 646     497     261      33
 
BtsI GCAGTG_nn'

Cuts at:      0     261     427     927    1437 
   Size:        261     166     500     510

  Fragments arranged by size: 

                 510     500     261     166
 
ClaI AT'CG_AT

Cuts at:      0     488    1437 
   Size:        488     949
 
EaeI y'GGCC_r

Cuts at:      0       2     260     789     924    1437 
   Size:          2     258     529     135     513

  Fragments arranged by size: 

                 529     513     258     135       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     602    1057    1437 
   Size:        602     455     380

  Fragments arranged by size: 

                 602     455     380
 
EcoICRI GAG'CTC

Cuts at:      0     957    1437 
   Size:        957     480
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      96     448     596     686     734     869     963     973 
   Size:         96     352     148      90      48     135      94      10

Cuts at:    973    1180    1289    1437 
   Size:        207     109     148

  Fragments arranged by size: 

                 352     207     148     148     135     109      96      94
                  90      48      10
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1191    1437 
   Size:       1191     246
 
EcoRI G'AATT_C

Cuts at:      0    1359    1437 
   Size:       1359      78
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      39     540    1188    1302    1437 
   Size:         39     501     648     114     135

  Fragments arranged by size: 

                 648     501     135     114      39
 
HaeII r_GCGC'y

Cuts at:      0    1050    1155    1297    1437 
   Size:       1050     105     142     140

  Fragments arranged by size: 

                1050     142     140     105
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      31      63     154     186     946     978    1011    1043 
   Size:         31      32      91      32     760      32      33      32

Cuts at:   1043    1437 
   Size:        394

  Fragments arranged by size: 

                 760     394      91      33      32      32      32      32
                  31
 
HincII GTy'rAC

Cuts at:      0     465    1437 
   Size:        465     972
 
KasI G'GCGC_C

Cuts at:      0    1293    1437 
   Size:       1293     144
 
MluI A'CGCG_T

Cuts at:      0     147    1437 
   Size:        147    1290
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     944     998    1437 
   Size:        944      54     439

  Fragments arranged by size: 

                 944     439      54
 
MscI TGG'CCA

Cuts at:      0       4     262     926    1437 
   Size:          4     258     664     511

  Fragments arranged by size: 

                 664     511     258       4
 
MslI CAynn'nnrTG

Cuts at:      0     199    1437 
   Size:        199    1238
 
MspA1I CmG'CkG

Cuts at:      0     983    1437 
   Size:        983     454
 
NarI GG'CG_CC

Cuts at:      0    1294    1437 
   Size:       1294     143
 
NcoI C'CATG_G

Cuts at:      0    1143    1437 
   Size:       1143     294
 
NdeI CA'TA_TG

Cuts at:      0     753    1409    1437 
   Size:        753     656      28

  Fragments arranged by size: 

                 753     656      28
 
NsiI A_TGCA'T

Cuts at:      0     659    1437 
   Size:        659     778
 
NspI r_CATG'y

Cuts at:      0     339     661    1437 
   Size:        339     322     776

  Fragments arranged by size: 

                 776     339     322
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     922    1437 
   Size:        922     515
 
PfoI T'CCnGG_A

Cuts at:      0     950    1437 
   Size:        950     487
 
PshAI GACnn'nnGTC

Cuts at:      0     857    1138    1437 
   Size:        857     281     299

  Fragments arranged by size: 

                 857     299     281
 
PvuII CAG'CTG

Cuts at:      0     983    1437 
   Size:        983     454
 
SacI G_AGCT'C

Cuts at:      0     959    1437 
   Size:        959     478
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1057    1437 
   Size:       1057     380
 
SfoI GGC'GCC

Cuts at:      0    1295    1437 
   Size:       1295     142
 
SmlI C'TyrA_G

Cuts at:      0     231    1437 
   Size:        231    1206
 
SphI G_CATG'C

Cuts at:      0     661    1437 
   Size:        661     776
 
StyI C'CwwG_G

Cuts at:      0     515    1136    1143    1437 
   Size:        515     621       7     294

  Fragments arranged by size: 

                 621     515     294       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1286    1437 
   Size:       1286     151
 
TatI w'GTAC_w

Cuts at:      0     403    1223    1437 
   Size:        403     820     214

  Fragments arranged by size: 

                 820     403     214
 
Tth111I GACn'n_nGTC

Cuts at:      0     102    1437 
   Size:        102    1335
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     487    1352    1437 
   Size:        487     865      85

  Fragments arranged by size: 

                 865     487      85
 
ZraI GAC'GTC

Cuts at:      0     462    1437 
   Size:        462     975
 

 Enzymes that do cut:

    AatII     AcuI     AfeI   AflIII     AgeI    AlwNI     ApoI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BfrBI     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI
    BsrBI    BsrFI    BsrGI   BstEII    BstXI    BstYI     BtgI     BtsI
     ClaI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
    HaeII    Hin4I   HincII     KasI     MluI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NsiI     NspI    PflMI     PfoI
    PshAI    PvuII     SacI     SapI     SfoI     SmlI     SphI     StyI
    TaqII     TatI  Tth111I     XcmI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI    AflII     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BciVI     BclI     BglI    BglII    BmgBI     BmtI     BplI
     BsaI    BsaAI    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrDI
   BssHII    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I    BtgZI    CspCI
     DraI   DraIII     DrdI     EagI     EciI    EcoNI    EcoRV     FseI
     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     NaeI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI     TstI     XbaI     XhoI     XmaI     XmnI
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