Restriction map for
pF1KB6502
(Circular) (Six-base) MAPSORT of: pF1KB6502.seq Check: 9282 from: 1 to: 4256
pF1KB6502 4256 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1223 1294 1223
Size: 71 4185
Acc65I G'GTAC_C
Cuts at: 1272 1272
Size: 4256
AclI AA'CG_TT
Cuts at: 4096 4096
Size: 4256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 2081 2513 3260 3528 498
Size: 1583 432 747 268 1226
Fragments arranged by size:
1583 1226 747 432 268
AflIII A'CryG_T
Cuts at: 785 1608 2712 3689 785
Size: 823 1104 977 1352
Fragments arranged by size:
1352 1104 977 823
AgeI A'CCGG_T
Cuts at: 2644 2644
Size: 4256
AhdI GACnn_n'nnGTC
Cuts at: 648 648
Size: 4256
AleI CACnn'nnGTG
Cuts at: 391 391
Size: 4256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 310 310
Size: 4256
AlwNI CAG_nnn'CTG
Cuts at: 419 1012 1128 1191 1561 3128 419
Size: 593 116 63 370 1567 1547
Fragments arranged by size:
1567 1547 593 370 116 63
ApaLI G'TGCA_C
Cuts at: 3026 3026
Size: 4256
ApoI r'AATT_y
Cuts at: 796 1260 3609 3865 796
Size: 464 2349 256 1187
Fragments arranged by size:
2349 1187 464 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4256
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
AvaI C'yCGr_G
Cuts at: 282 451 607 953 1276 282
Size: 169 156 346 323 3262
Fragments arranged by size:
3262 346 323 169 156
BamHI G'GATC_C
Cuts at: 1281 1281
Size: 4256
BanI G'GyrC_C
Cuts at: 299 379 1272 1686 1934 1969 299
Size: 80 893 414 248 35 2586
Fragments arranged by size:
2586 893 414 248 80 35
BanII G_rGCy'C
Cuts at: 608 1270 2300 608
Size: 662 1030 2564
Fragments arranged by size:
2564 1030 662
BbeI G_GCGC'C
Cuts at: 303 1690 1938 303
Size: 1387 248 2621
Fragments arranged by size:
2621 1387 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 668 702 668
Size: 34 4222
BciVI GTATCCnnnnn_n'
Cuts at: 2154 2915 4256 2154
Size: 761 1341 2154
Fragments arranged by size:
2154 1341 761
BclI T'GATC_A
Cuts at: 1776 1776
Size: 4256
BglII A'GATC_T
Cuts at: 734 1771 3808 734
Size: 1037 2037 1182
Fragments arranged by size:
2037 1182 1037
BlpI GC'TnA_GC
Cuts at: 1368 1368
Size: 4256
Bme1580I G_kGCm'C
Cuts at: 134 1881 1974 3030 134
Size: 1747 93 1056 1360
Fragments arranged by size:
1747 1360 1056 93
BmgBI CAC'GTC
Cuts at: 786 786
Size: 4256
BmrI ACTGGGnnnn_n'
Cuts at: 721 909 1468 1549 1884 4028 721
Size: 188 559 81 335 2144 949
Fragments arranged by size:
2144 949 559 335 188 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2623 2623
Size: 4256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 918 208
Size: 710 3546
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 457 1432 2311 2803 3101 448
Size: 9 975 879 492 298 1603
Fragments arranged by size:
1603 975 879 492 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 544 659 3960 544
Size: 115 3301 840
Fragments arranged by size:
3301 840 115
BsaAI yAC'GTr
Cuts at: 1609 1609
Size: 4256
BsaBI GATnn'nnATC
Cuts at: 6 1770 3807 6
Size: 1764 2037 455
Fragments arranged by size:
2037 1764 455
BsaHI Gr'CG_yC
Cuts at: 300 1687 1935 2637 4133 300
Size: 1387 248 702 1496 423
Fragments arranged by size:
1496 1387 702 423 248
BsaWI w'CCGG_w
Cuts at: 1445 1966 2644 2918 3065 3476 1445
Size: 521 678 274 147 411 2225
Fragments arranged by size:
2225 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 192 600 1146 1681 2439 3016 3512 192
Size: 408 546 535 758 577 496 936
Fragments arranged by size:
936 758 577 546 535 496 408
BsiEI CG_ry'CG
Cuts at: 104 229 1798 1844 3052 104
Size: 125 1569 46 1208 1308
Fragments arranged by size:
1569 1308 1208 125 46
BsiHKAI G_wGCw'C
Cuts at: 398 1270 2048 2238 3030 398
Size: 872 778 190 792 1624
Fragments arranged by size:
1624 872 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 134 398 608 1270 1881 1974 2048 2238 2300
Size: 264 210 662 611 93 74 190 62
Cuts at: 2300 3030 134
Size: 730 1360
Fragments arranged by size:
1360 730 662 611 264 210 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3476 3476
Size: 4256
BspHI T'CATG_A
Cuts at: 3432 3432
Size: 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 326 1306 1520 1822 2203 326
Size: 980 214 302 381 2379
Fragments arranged by size:
2379 980 381 302 214
BsrBI CCG'CTC
Cuts at: 50 204 1004 2548 2602 4086 4251 50
Size: 154 800 1544 54 1484 165 55
Fragments arranged by size:
1544 1484 800 165 154 55 54
BsrDI GCAATG_nn'
Cuts at: 543 981 2168 543
Size: 438 1187 2631
Fragments arranged by size:
2631 1187 438
BsrFI r'CCGG_y
Cuts at: 224 321 2254 2435 2644 224
Size: 97 1933 181 209 1836
Fragments arranged by size:
1933 1836 209 181 97
BssHII G'CGCG_C
Cuts at: 327 329 327
Size: 2 4254
BssSI C'ACGA_G
Cuts at: 567 2527 2885 567
Size: 1960 358 1938
Fragments arranged by size:
1960 1938 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1633 1633
Size: 4256
BstBI TT'CG_AA
Cuts at: 2617 2617
Size: 4256
BstEII G'GTnAC_C
Cuts at: 313 313
Size: 4256
BstXI CCAn_nnnn'nTGG
Cuts at: 273 780 273
Size: 507 3749
BstYI r'GATC_y
Cuts at: 1 734 1281 1748 1771 2106 2352 3364 3450
Size: 733 547 467 23 335 246 1012 86
Cuts at: 3450 3462 3808 1
Size: 12 346 449
Fragments arranged by size:
1012 733 547 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1224 1224
Size: 4256
Bsu36I CC'TnA_GG
Cuts at: 926 926
Size: 4256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2206 2387 2550 2206
Size: 181 163 3912
Fragments arranged by size:
3912 181 163
BtsI GCAGTG_nn'
Cuts at: 1499 1499
Size: 4256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 948 983 948
Size: 35 4221
DraI TTT'AAA
Cuts at: 867 1255 867
Size: 388 3868
DrdI GACnn_nn'nnGTC
Cuts at: 1962 2820 1962
Size: 858 3398
EaeI y'GGCC_r
Cuts at: 226 263 275 1841 2015 2406 2433 3778 226
Size: 37 12 1566 174 391 27 1345 704
Fragments arranged by size:
1566 1345 704 391 174 37 27 12
EagI C'GGCC_G
Cuts at: 226 1841 226
Size: 1615 2641
EarI CTCTTCn'nnn_
Cuts at: 340 639 2279 2489 340
Size: 299 1640 210 2107
Fragments arranged by size:
2107 1640 299 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2464 2774 2920 4066 2464
Size: 310 146 1146 2654
Fragments arranged by size:
2654 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1268 1268
Size: 4256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 498 918 2081 2513 3260 3528 208
Size: 290 420 1163 432 747 268 936
Fragments arranged by size:
1163 936 747 432 420 290 268
EcoNI CCTnn'n_nnAGG
Cuts at: 307 307
Size: 4256
EcoO109I rG'GnC_Cy
Cuts at: 612 1395 612
Size: 783 3473
EcoRI G'AATT_C
Cuts at: 796 1260 796
Size: 464 3792
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 831 2275 831
Size: 1444 2812
FspI TGC'GCA
Cuts at: 2037 2037
Size: 4256
HaeII r_GCGC'y
Cuts at: 303 375 1690 1938 2960 3939 303
Size: 72 1315 248 1022 979 620
Fragments arranged by size:
1315 1022 979 620 248 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 622 634 654 666 2098 2128 2130 2160 622
Size: 12 20 12 1432 30 2 30 2718
Fragments arranged by size:
2718 1432 30 30 20 12 12 2
HincII GTy'rAC
Cuts at: 1295 1295
Size: 4256
KasI G'GCGC_C
Cuts at: 299 1686 1934 299
Size: 1387 248 2621
Fragments arranged by size:
2621 1387 248
KpnI G_GTAC'C
Cuts at: 1276 1276
Size: 4256
MluI A'CGCG_T
Cuts at: 3689 3689
Size: 4256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 122 731 770 1115 1235 2927 3111 122
Size: 609 39 345 120 1692 184 1267
Fragments arranged by size:
1692 1267 609 345 184 120 39
MscI TGG'CCA
Cuts at: 277 2017 277
Size: 1740 2516
MslI CAynn'nnrTG
Cuts at: 271 391 2245 2372 271
Size: 120 1854 127 2155
Fragments arranged by size:
2155 1854 127 120
MspA1I CmG'CkG
Cuts at: 186 702 1125 1191 1367 1681 2041 3054 3299
Size: 516 423 66 176 314 360 1013 245
Cuts at: 3299 186
Size: 1143
Fragments arranged by size:
1143 1013 516 423 360 314 245 176
66
NaeI GCC'GGC
Cuts at: 226 2437 226
Size: 2211 2045
NarI GG'CG_CC
Cuts at: 300 1687 1935 300
Size: 1387 248 2621
Fragments arranged by size:
2621 1387 248
NgoMIV G'CCGG_C
Cuts at: 224 2435 224
Size: 2211 2045
NspI r_CATG'y
Cuts at: 1309 1809 2716 1309
Size: 500 907 2849
Fragments arranged by size:
2849 907 500
PciI A'CATG_T
Cuts at: 2712 2712
Size: 4256
PmeI GTTT'AAAC
Cuts at: 1255 1255
Size: 4256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 3395
Size: 32 4224
PstI C_TGCA'G
Cuts at: 308 1303 1988 308
Size: 995 685 2576
Fragments arranged by size:
2576 995 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4256
PvuII CAG'CTG
Cuts at: 186 1125 1191 1681 2041 186
Size: 939 66 490 360 2401
Fragments arranged by size:
2401 939 490 360 66
RsrII CG'GwC_CG
Cuts at: 2451 2451
Size: 4256
SacI G_AGCT'C
Cuts at: 1270 1270
Size: 4256
SalI G'TCGA_C
Cuts at: 1293 1293
Size: 4256
SapI GCTCTTCn'nnn_
Cuts at: 340 2279 2489 340
Size: 1939 210 2107
Fragments arranged by size:
2107 1939 210
SbfI CC_TGCA'GG
Cuts at: 1303 1303
Size: 4256
ScaI AGT'ACT
Cuts at: 3773 3773
Size: 4256
SexAI A'CCwGG_T
Cuts at: 1226 1226
Size: 4256
SfcI C'TryA_G
Cuts at: 33 304 1299 1984 2977 3168 33
Size: 271 995 685 993 191 1121
Fragments arranged by size:
1121 995 993 685 271 191
SfoI GGC'GCC
Cuts at: 301 1688 1936 301
Size: 1387 248 2621
Fragments arranged by size:
2621 1387 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
SmaI CCC'GGG
Cuts at: 284 955 1278 284
Size: 671 323 3262
Fragments arranged by size:
3262 671 323
SmlI C'TyrA_G
Cuts at: 463 472 1411 2326 2818 3080 463
Size: 9 939 915 492 262 1639
Fragments arranged by size:
1639 939 915 492 262 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4256
SphI G_CATG'C
Cuts at: 1309 1809 1309
Size: 500 3756
StyI C'CwwG_G
Cuts at: 1390 1744 1390
Size: 354 3902
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 677 1839 1972 2431 2640 677
Size: 1162 133 459 209 2293
Fragments arranged by size:
2293 1162 459 209 133
TatI w'GTAC_w
Cuts at: 809 3771 809
Size: 2962 1294
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3773 3805 3773
Size: 32 4224
Tth111I GACn'n_nGTC
Cuts at: 1072 2053 1072
Size: 981 3275
XbaI T'CTAG_A
Cuts at: 558 1287 558
Size: 729 3527
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1751 1751
Size: 4256
XmaI C'CCGG_G
Cuts at: 282 953 1276 282
Size: 671 323 3262
Fragments arranged by size:
3262 671 323
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AloI ApaI AscI AvrII
BaeI BbsI BbvCI BfrBI BglI BmtI Bpu10I BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BtgI ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI