Restriction map for pF1KB3134
 (Linear) (Six-base) MAPSORT of: KIBB3134.seq  Check: 6683  from: 1  to: 3690



KIBB3134.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1692    3690 
   Size:       1692    1998
 
Acc65I G'GTAC_C

Cuts at:      0      24    3690 
   Size:         24    3666
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307    3690 
   Size:        307    3383
 
AleI CACnn'nnGTG

Cuts at:      0    3409    3690 
   Size:       3409     281
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1366    1398    2708    2740    3010    3042    3690 
   Size:       1366      32    1310      32     270      32     648

  Fragments arranged by size: 

                1366    1310     648     270      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     742    2536    3369    3690 
   Size:        742    1794     833     321

  Fragments arranged by size: 

                1794     833     742     321
 
ApoI r'AATT_y

Cuts at:      0     225     287    2348    2755    3690 
   Size:        225      62    2061     407     935

  Fragments arranged by size: 

                2061     935     407     225      62
 
AseI AT'TA_AT

Cuts at:      0    2072    3690 
   Size:       2072    1618
 
AvaI C'yCGr_G

Cuts at:      0    1543    3239    3690 
   Size:       1543    1696     451

  Fragments arranged by size: 

                1696    1543     451
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2586    2619    3690 
   Size:       2586      33    1071

  Fragments arranged by size: 

                2586    1071      33
 
BamHI G'GATC_C

Cuts at:      0    1492    2198    3690 
   Size:       1492     706    1492

  Fragments arranged by size: 

                1492    1492     706
 
BanI G'GyrC_C

Cuts at:      0      24    1113    3690 
   Size:         24    1089    2577

  Fragments arranged by size: 

                2577    1089      24
 
BanII G_rGCy'C

Cuts at:      0    1283    1311    1892    3254    3690 
   Size:       1283      28     581    1362     436

  Fragments arranged by size: 

                1362    1283     581     436      28
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     803    1679    1965    2261    3326    3690 
   Size:        803     876     286     296    1065     364

  Fragments arranged by size: 

                1065     876     803     364     296     286
 
BbvCI CC'TCA_GC

Cuts at:      0    1343    1478    1660    2858    3260    3690 
   Size:       1343     135     182    1198     402     430

  Fragments arranged by size: 

                1343    1198     430     402     182     135
 
BclI T'GATC_A

Cuts at:      0      87     146    1484    3690 
   Size:         87      59    1338    2206

  Fragments arranged by size: 

                2206    1338      87      59
 
BglII A'GATC_T

Cuts at:      0    3059    3114    3510    3690 
   Size:       3059      55     396     180

  Fragments arranged by size: 

                3059     396     180      55
 
BlpI GC'TnA_GC

Cuts at:      0     395    3248    3690 
   Size:        395    2853     442

  Fragments arranged by size: 

                2853     442     395
 
Bme1580I G_kGCm'C

Cuts at:      0    1832    3690 
   Size:       1832    1858
 
BmgBI CAC'GTC

Cuts at:      0    1602    1784    3690 
   Size:       1602     182    1906

  Fragments arranged by size: 

                1906    1602     182
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     189    2481    3690 
   Size:        189    2292    1209

  Fragments arranged by size: 

                2292    1209     189
 
BmtI G_CTAG'C

Cuts at:      0    2906    2914    3690 
   Size:       2906       8     776

  Fragments arranged by size: 

                2906     776       8
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3059    3690 
   Size:       3059     631
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2122    3358    3391    3690 
   Size:       2122    1236      33     299

  Fragments arranged by size: 

                2122    1236     299      33
 
Bpu10I CC'TnA_GC

Cuts at:      0     383    1343    1478    1660    2858    3034    3260    3358 
   Size:        383     960     135     182    1198     176     226      98

Cuts at:   3358    3690 
   Size:        332

  Fragments arranged by size: 

                1198     960     383     332     226     182     176     135
                  98
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     448    1237    1447    2010    2017    3139    3690 
   Size:        148     300     789     210     563       7    1122     551

  Fragments arranged by size: 

                1122     789     563     551     300     210     148       7
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1405    3690 
   Size:       1405    2285
 
BsaBI GATnn'nnATC

Cuts at:      0     148     872    3310    3690 
   Size:        148     724    2438     380

  Fragments arranged by size: 

                2438     724     380     148
 
BsaHI Gr'CG_yC

Cuts at:      0     189    3690 
   Size:        189    3501
 
BsaWI w'CCGG_w

Cuts at:      0     329    2408    3690 
   Size:        329    2079    1282

  Fragments arranged by size: 

                2079    1282     329
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1366    1396    2187    2217    2708    2738    3010    3040 
   Size:       1366      30     791      30     491      30     272      30

Cuts at:   3040    3331    3361    3690 
   Size:        291      30     329

  Fragments arranged by size: 

                1366     791     491     329     291     272      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1315    1360    1507    1580    3565    3690 
   Size:       1315      45     147      73    1985     125

  Fragments arranged by size: 

                1985    1315     147     125      73      45
 
BseYI C'CCAG_C

Cuts at:      0    1865    2319    3690 
   Size:       1865     454    1371

  Fragments arranged by size: 

                1865    1371     454
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1033    1363    2497    2830    3690 
   Size:       1033     330    1134     333     860

  Fragments arranged by size: 

                1134    1033     860     333     330
 
BsiHKAI G_wGCw'C

Cuts at:      0    1892    2114    3254    3690 
   Size:       1892     222    1140     436

  Fragments arranged by size: 

                1892    1140     436     222
 
BsmI GAATG_Cn'

Cuts at:      0     635    1637    3690 
   Size:        635    1002    2053

  Fragments arranged by size: 

                2053    1002     635
 
Bsp1286I G_dGCh'C

Cuts at:      0    1283    1311    1832    1892    2114    3254    3690 
   Size:       1283      28     521      60     222    1140     436

  Fragments arranged by size: 

                1283    1140     521     436     222      60      28
 
BspEI T'CCGG_A

Cuts at:      0    2408    3690 
   Size:       2408    1282
 
BspHI T'CATG_A

Cuts at:      0    1205    3665    3690 
   Size:       1205    2460      25

  Fragments arranged by size: 

                2460    1205      25
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      36     390    1385    1692    2810    3690 
   Size:         36     354     995     307    1118     880

  Fragments arranged by size: 

                1118     995     880     354     307      36
 
BsrDI GCAATG_nn'

Cuts at:      0    2937    3254    3690 
   Size:       2937     317     436

  Fragments arranged by size: 

                2937     436     317
 
BsrFI r'CCGG_y

Cuts at:      0    1106    3690 
   Size:       1106    2584
 
BsrGI T'GTAC_A

Cuts at:      0    1158    3690 
   Size:       1158    2532
 
BssSI C'ACGA_G

Cuts at:      0    1846    3690 
   Size:       1846    1844
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2011    2536    3690 
   Size:       2011     525    1154

  Fragments arranged by size: 

                2011    1154     525
 
BstBI TT'CG_AA

Cuts at:      0    2760    3690 
   Size:       2760     930
 
BstEII G'GTnAC_C

Cuts at:      0     121    3690 
   Size:        121    3569
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     495    3690 
   Size:        495    3195
 
BstYI r'GATC_y

Cuts at:      0     910    1492    1983    2198    2935    3059    3114    3510 
   Size:        910     582     491     215     737     124      55     396

Cuts at:   3510    3690 
   Size:        180

  Fragments arranged by size: 

                 910     737     582     491     396     215     180     124
                  55
 
Bsu36I CC'TnA_GG

Cuts at:      0    1298    3690 
   Size:       1298    2392
 
BtgI C'CryG_G

Cuts at:      0    1445    1505    2051    2492    2684    3497    3690 
   Size:       1445      60     546     441     192     813     193

  Fragments arranged by size: 

                1445     813     546     441     193     192      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3658    3690 
   Size:       3658      32
 
BtsI GCAGTG_nn'

Cuts at:      0    3193    3690 
   Size:       3193     497
 
DraI TTT'AAA

Cuts at:      0    2217    3690 
   Size:       2217    1473
 
DraIII CAC_nnn'GTG

Cuts at:      0    2520    3690 
   Size:       2520    1170
 
EaeI y'GGCC_r

Cuts at:      0     497    1706    3690 
   Size:        497    1209    1984

  Fragments arranged by size: 

                1984    1209     497
 
EarI CTCTTCn'nnn_

Cuts at:      0    2464    3690 
   Size:       2464    1226
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     499    3690 
   Size:        499    3191
 
EcoICRI GAG'CTC

Cuts at:      0    1890    3252    3690 
   Size:       1890    1362     438

  Fragments arranged by size: 

                1890    1362     438
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307    2122    3358    3391    3690 
   Size:        307    1815    1236      33     299

  Fragments arranged by size: 

                1815    1236     307     299      33
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1121    1173    1253    3690 
   Size:       1121      52      80    2437

  Fragments arranged by size: 

                2437    1121      80      52
 
EcoO109I rG'GnC_Cy

Cuts at:      0     445    1010    1166    1795    1971    3690 
   Size:        445     565     156     629     176    1719

  Fragments arranged by size: 

                1719     629     565     445     176     156
 
EcoRI G'AATT_C

Cuts at:      0     287    3690 
   Size:        287    3403
 
EcoRV GAT'ATC

Cuts at:      0     135    2058    3690 
   Size:        135    1923    1632

  Fragments arranged by size: 

                1923    1632     135
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     337    3690 
   Size:        337    3353
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     231     263    2941    2973    3690 
   Size:        231      32    2678      32     717

  Fragments arranged by size: 

                2678     717     231      32      32
 
HincII GTy'rAC

Cuts at:      0    2127    3280    3690 
   Size:       2127    1153     410

  Fragments arranged by size: 

                2127    1153     410
 
HindIII A'AGCT_T

Cuts at:      0     424    1693    3690 
   Size:        424    1269    1997

  Fragments arranged by size: 

                1997    1269     424
 
KpnI G_GTAC'C

Cuts at:      0      28    3690 
   Size:         28    3662
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     179    2728    3467    3690 
   Size:        179    2549     739     223

  Fragments arranged by size: 

                2549     739     223     179
 
MscI TGG'CCA

Cuts at:      0     499    3690 
   Size:        499    3191
 
MslI CAynn'nnrTG

Cuts at:      0     866    1604    3409    3690 
   Size:        866     738    1805     281

  Fragments arranged by size: 

                1805     866     738     281
 
MspA1I CmG'CkG

Cuts at:      0     217    1086    1387    1731    2002    3336    3366    3690 
   Size:        217     869     301     344     271    1334      30     324

  Fragments arranged by size: 

                1334     869     344     324     301     271     217      30
            
 
NaeI GCC'GGC

Cuts at:      0    1108    3690 
   Size:       1108    2582
 
NcoI C'CATG_G

Cuts at:      0    2051    2492    2684    3497    3690 
   Size:       2051     441     192     813     193

  Fragments arranged by size: 

                2051     813     441     193     192
 
NgoMIV G'CCGG_C

Cuts at:      0    1106    3690 
   Size:       1106    2584
 
NheI G'CTAG_C

Cuts at:      0    2902    2910    3690 
   Size:       2902       8     780

  Fragments arranged by size: 

                2902     780       8
 
NspI r_CATG'y

Cuts at:      0    2009    3690 
   Size:       2009    1681
 
PasI CC'CwG_GG

Cuts at:      0    1799    3690 
   Size:       1799    1891
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2052    2493    2498    2584    3690 
   Size:       2052     441       5      86    1106

  Fragments arranged by size: 

                2052    1106     441      86       5
 
PfoI T'CCnGG_A

Cuts at:      0     563    1427    3314    3690 
   Size:        563     864    1887     376

  Fragments arranged by size: 

                1887     864     563     376
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1366    1398    2708    2740    3010    3042    3690 
   Size:       1366      32    1310      32     270      32     648

  Fragments arranged by size: 

                1366    1310     648     270      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     445    1010    1795    3690 
   Size:        445     565     785    1895

  Fragments arranged by size: 

                1895     785     565     445
 
PshAI GACnn'nnGTC

Cuts at:      0    1754    3449    3690 
   Size:       1754    1695     241

  Fragments arranged by size: 

                1754    1695     241
 
PsiI TTA'TAA

Cuts at:      0      97    3690 
   Size:         97    3593
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     252     284    3690 
   Size:        252      32    3406

  Fragments arranged by size: 

                3406     252      32
 
PstI C_TGCA'G

Cuts at:      0     579    1064    1703    1967    2066    2532    3690 
   Size:        579     485     639     264      99     466    1158

  Fragments arranged by size: 

                1158     639     579     485     466     264      99
 
PvuII CAG'CTG

Cuts at:      0    1086    1731    2002    3336    3366    3690 
   Size:       1086     645     271    1334      30     324

  Fragments arranged by size: 

                1334    1086     645     324     271      30
 
SacI G_AGCT'C

Cuts at:      0    1892    3254    3690 
   Size:       1892    1362     436

  Fragments arranged by size: 

                1892    1362     436
 
SanDI GG'GwC_CC

Cuts at:      0     445    1795    3690 
   Size:        445    1350    1895

  Fragments arranged by size: 

                1895    1350     445
 
ScaI AGT'ACT

Cuts at:      0    1212    3690 
   Size:       1212    2478
 
SfcI C'TryA_G

Cuts at:      0     575     763    1060    1081    1699    1963    2062    2528 
   Size:        575     188     297      21     618     264      99     466

Cuts at:   2528    3433    3690 
   Size:        905     257

  Fragments arranged by size: 

                 905     618     575     466     297     264     257     188
                  99      21
 
SmaI CCC'GGG

Cuts at:      0    3241    3690 
   Size:       3241     449
 
SmlI C'TyrA_G

Cuts at:      0     127     427    1216    1462    1543    1989    2032    3118 
   Size:        127     300     789     246      81     446      43    1086

Cuts at:   3118    3690 
   Size:        572

  Fragments arranged by size: 

                1086     789     572     446     300     246     127      81
                  43
 
SspI AAT'ATT

Cuts at:      0    1232    2695    3690 
   Size:       1232    1463     995

  Fragments arranged by size: 

                1463    1232     995
 
StuI AGG'CCT

Cuts at:      0    3185    3690 
   Size:       3185     505
 
StyI C'CwwG_G

Cuts at:      0     803     843    2015    2051    2492    2684    2738    3497 
   Size:        803      40    1172      36     441     192      54     759

Cuts at:   3497    3690 
   Size:        193

  Fragments arranged by size: 

                1172     803     759     441     193     192      54      40
                  36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     109    1733    2353    3490    3690 
   Size:        109    1624     620    1137     200

  Fragments arranged by size: 

                1624    1137     620     200     109
 
TatI w'GTAC_w

Cuts at:      0     309    1158    1210    2261    3690 
   Size:        309     849      52    1051    1429

  Fragments arranged by size: 

                1429    1051     849     309      52
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1762    1794    2187    2219    3690 
   Size:       1762      32     393      32    1471

  Fragments arranged by size: 

                1762    1471     393      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1720    3690 
   Size:       1720    1970
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1452    1750    3690 
   Size:       1452     298    1940

  Fragments arranged by size: 

                1940    1452     298
 
XhoI C'TCGA_G

Cuts at:      0    1543    3690 
   Size:       1543    2147
 
XmaI C'CCGG_G

Cuts at:      0    3239    3690 
   Size:       3239     451
 
XmnI GAAnn'nnTTC

Cuts at:      0     287     320     719    2723    3512    3690 
   Size:        287      33     399    2004     789     178

  Fragments arranged by size: 

                2004     789     399     287     178      33
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AleI     AloI    AlwNI     ApoI     AseI
     AvaI     BaeI    BamHI     BanI    BanII     BbsI    BbvCI     BclI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI    Hin4I   HincII  HindIII     KpnI     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NheI     NspI     PasI    PflMI
     PfoI     PpiI    PpuMI    PshAI     PsiI     PsrI     PstI    PvuII
     SacI    SanDI     ScaI     SfcI     SmaI     SmlI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     ApaI    ApaLI     AscI    AsiSI    AvrII     BbeI     BcgI
    BciVI    BfrBI     BglI    BsaAI    BsiEI    BsiWI    BsmBI    BsrBI
   BssHII  BstZ17I     ClaI    CspCI     DrdI     EagI     FseI     FspI
    FspAI    HaeII     HpaI     KasI     MfeI     MluI     NarI     NdeI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI   PspOMI
    PspXI     PvuI    RsrII    SacII     SalI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SwaI     XbaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com