Restriction map for pF1KB8679
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
AocI        Aor51HI     AscI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BbeI        BbrPI       BbvCI       BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MunI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AfaI          365  GTAC                 GT^AC
AflIII        143  ACACGT               A^C[GA][CT]GT
Alw44I        691  GTGCAC               G^TGCAC
AlwNI         808  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          512  GGGCCC               GGGCC^C
ApaLI         691  GTGCAC               G^TGCAC
Asp700I       100  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         691  GTGCAC               G[AT]GC[AT]^C
BamHI         372  GGATCC               G^GATCC
BanII         512  GGGCCC               G[GA]GC[CT]^C
BbsI          348  GAAGAC               GAAGAC(2/6)
BcgI          729  GCAGGAGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         332  GGATAC               GTATCC(6/5)
BpuAI         348  GAAGAC               GAAGAC(2/6)
BsiHKAI       691  GTGCAC               G[AT]GC[AT]^C
BsmBI         146  CGTCTC               CGTCTC(1/5)
BspHI         758  TCATGA               T^CATGA
BsrDI          62  CATTGC               GCAATG(2/0)
BsrI          385  ACTGG                ACTGG(1/-1)
BstXI         416  CCACTCGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         372  GGATCC               [GA]^GATC[CT]
DraIII        775  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1077  CCATGG               C^C[GA][CT]GG
EcoT22I       787  ATGCAT               ATGCA^T
HapII         471  CCGG                 C^CGG
HpaII         471  CCGG                 C^CGG
MaeII         145  ACGT                 A^CGT
MflI          372  GGATCC               [GA]^GATC[CT]
MslI          826  CACAAGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1055  CAGCGG               C[AC]G^C[GT]G
MspI          471  CCGG                 C^CGG
NcoI         1077  CCATGG               C^CATGG
NsiI          787  ATGCAT               ATGCA^T
NspI          217  GCATGT               [GA]CATG^[CT]
PflMI         306  CCATGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         496  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          563  CTGCAG               CTGCA^G
RcaI          758  TCATGA               T^CATGA
RsaI          365  GTAC                 GT^AC
Van91I        306  CCATGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         372  GGATCC               [GA]^GATC[CT]
XmnI          100  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AcsI          103  GAATTT               [GA]^AATT[CT]
              171  GAATTT              
ApoI          103  GAATTT               [GA]^AATT[CT]
              171  GAATTT              
AseI          274  ATTAAT               AT^TAAT
             1044  ATTAAT              
AsnI          274  ATTAAT               AT^TAAT
             1044  ATTAAT              
BanI          532  GGTGCC               G^G[CT][GA]CC
              667  GGCACC              
BfaI          509  CTAG                 C^TAG
              517  CTAG                
BseRI        1373  CTCCTC               GAGGAG(10/8)
             1385  CTCCTC              
BsrBI          52  GAGCGG               CCGCTC(-3/-3)
             1452  GAGCGG              
BstF5I          6  GGATG                GGATG(2/0)
              831  GGATG               
Cac8I         445  GCAGGC               GC[ACGT]^[ACGT]GC
              664  GCTGGC              
FauI         1302  CCCGC                CCCGC(4/6)
             1454  GCGGG               
FokI            6  GGATG                GGATG(9/13)
              831  GGATG               
HincII        876  GTTGAC               GT[CT]^[GA]AC
             1249  GTTGAC              
HindII        876  GTTGAC               GT[CT]^[GA]AC
             1249  GTTGAC              
MaeI          509  CTAG                 C^TAG
              517  CTAG                
PshBI         274  ATTAAT               AT^TAAT
             1044  ATTAAT              
SfcI          563  CTGCAG               C^T[GA][CT]AG
             1254  CTACAG              
TaqI          327  TCGA                 T^CGA
              963  TCGA                
TthHB8I       327  TCGA                 T^CGA
              963  TCGA                
XcmI         1268  CCAAAAAAGAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1436  CCAAAGCTGGTCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BbvI        
BclI        BmyI        BpmI        BsaJI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BssKI       BstAPI      BstNI       Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      EcoT14I     FbaI        Fnu4HI      HaeIII      
HinfI       HphI        ItaI        Ksp632I     MaeIII      
MboI        MboII       MnlI        MseI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       
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