Restriction map for pF1KB8679
 (Linear) (Six-base) MAPSORT of: KIBB8679.seq  Check: 6405  from: 1  to: 1542



KIBB8679.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1233    1542 
   Size:       1233     309
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     256    1307    1542 
   Size:        119     137    1051     235

  Fragments arranged by size: 

                1051     235     137     119
 
AflIII A'CryG_T

Cuts at:      0     143    1542 
   Size:        143    1399
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1328    1542 
   Size:       1328     214
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     732     764    1542 
   Size:        732      32     778

  Fragments arranged by size: 

                 778     732      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     813    1542 
   Size:        813     729
 
ApaI G_GGCC'C

Cuts at:      0     516    1542 
   Size:        516    1026
 
ApaLI G'TGCA_C

Cuts at:      0     691    1542 
   Size:        691     851
 
ApoI r'AATT_y

Cuts at:      0     103     171    1542 
   Size:        103      68    1371

  Fragments arranged by size: 

                1371     103      68
 
AseI AT'TA_AT

Cuts at:      0     275    1045    1542 
   Size:        275     770     497

  Fragments arranged by size: 

                 770     497     275
 
BamHI G'GATC_C

Cuts at:      0     372    1542 
   Size:        372    1170
 
BanI G'GyrC_C

Cuts at:      0     532     667    1542 
   Size:        532     135     875

  Fragments arranged by size: 

                 875     532     135
 
BanII G_rGCy'C

Cuts at:      0     516    1542 
   Size:        516    1026
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     355    1542 
   Size:        355    1187
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     718     752    1542 
   Size:        718      34     790

  Fragments arranged by size: 

                 790     718      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     326    1542 
   Size:        326    1216
 
BclI T'GATC_A

Cuts at:      0    1106    1175    1394    1542 
   Size:       1106      69     219     148

  Fragments arranged by size: 

                1106     219     148      69
 
BfrBI ATG'CAT

Cuts at:      0     789    1542 
   Size:        789     753
 
Bme1580I G_kGCm'C

Cuts at:      0     359     516     695    1542 
   Size:        359     157     179     847

  Fragments arranged by size: 

                 847     359     179     157
 
BmgBI CAC'GTC

Cuts at:      0     146    1542 
   Size:        146    1396
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     773    1361    1468    1487    1542 
   Size:        773     588     107      19      55

  Fragments arranged by size: 

                 773     588     107      55      19
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      43      73     732     762    1542 
   Size:         43      30     659      30     780

  Fragments arranged by size: 

                 780     659      43      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1364    1376    1542 
   Size:       1364      12     166

  Fragments arranged by size: 

                1364     166      12
 
BseYI C'CCAG_C

Cuts at:      0     500    1542 
   Size:        500    1042
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     460     464     932    1542 
   Size:        460       4     468     610

  Fragments arranged by size: 

                 610     468     460       4
 
BsiHKAI G_wGCw'C

Cuts at:      0     695    1542 
   Size:        695     847
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     152    1542 
   Size:        152    1390
 
Bsp1286I G_dGCh'C

Cuts at:      0     359     516     695    1542 
   Size:        359     157     179     847

  Fragments arranged by size: 

                 847     359     179     157
 
BspHI T'CATG_A

Cuts at:      0     758    1542 
   Size:        758     784
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     851    1078    1233    1296    1542 
   Size:        851     227     155      63     246

  Fragments arranged by size: 

                 851     246     227     155      63
 
BsrBI CCG'CTC

Cuts at:      0      54    1454    1542 
   Size:         54    1400      88

  Fragments arranged by size: 

                1400      88      54
 
BsrDI GCAATG_nn'

Cuts at:      0      61    1542 
   Size:         61    1481
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     795     919    1330    1542 
   Size:        795     124     411     212

  Fragments arranged by size: 

                 795     411     212     124
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     423    1542 
   Size:        423    1119
 
BstYI r'GATC_y

Cuts at:      0     372    1542 
   Size:        372    1170
 
BtgI C'CryG_G

Cuts at:      0    1077    1542 
   Size:       1077     465
 
DraIII CAC_nnn'GTG

Cuts at:      0     780    1542 
   Size:        780     762
 
EarI CTCTTCn'nnn_

Cuts at:      0     232     306    1408    1542 
   Size:        232      74    1102     134

  Fragments arranged by size: 

                1102     232     134      74
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1542 
   Size:         19    1523
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     256     773    1307    1361    1468    1487    1542 
   Size:        119     137     517     534      54     107      19      55

  Fragments arranged by size: 

                 534     517     137     119     107      55      54      19
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     497     512     513    1185    1542 
   Size:        497      15       1     672     357

  Fragments arranged by size: 

                 672     497     357      15       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     124    1542 
   Size:        124    1418
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      43      75     274     306     626     658    1010    1042 
   Size:         43      32     199      32     320      32     352      32

Cuts at:   1042    1542 
   Size:        500

  Fragments arranged by size: 

                 500     352     320     199      43      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     878    1251    1542 
   Size:        878     373     291

  Fragments arranged by size: 

                 878     373     291
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     119    1542 
   Size:        119    1423
 
MslI CAynn'nnrTG

Cuts at:      0     830    1542 
   Size:        830     712
 
MspA1I CmG'CkG

Cuts at:      0    1057    1542 
   Size:       1057     485
 
NcoI C'CATG_G

Cuts at:      0    1077    1542 
   Size:       1077     465
 
NsiI A_TGCA'T

Cuts at:      0     791    1542 
   Size:        791     751
 
NspI r_CATG'y

Cuts at:      0     221    1542 
   Size:        221    1321
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     312    1542 
   Size:        312    1230
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     732     764    1542 
   Size:        732      32     778

  Fragments arranged by size: 

                 778     732      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     497    1542 
   Size:        497    1045
 
PspOMI G'GGCC_C

Cuts at:      0     512    1542 
   Size:        512    1030
 
PstI C_TGCA'G

Cuts at:      0     567    1542 
   Size:        567     975
 
SanDI GG'GwC_CC

Cuts at:      0     497    1542 
   Size:        497    1045
 
SfcI C'TryA_G

Cuts at:      0     563    1254    1542 
   Size:        563     691     288

  Fragments arranged by size: 

                 691     563     288
 
StyI C'CwwG_G

Cuts at:      0     377     696    1077    1542 
   Size:        377     319     381     465

  Fragments arranged by size: 

                 465     381     377     319
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     639    1542 
   Size:        639     903
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     672     704    1542 
   Size:        672      32     838

  Fragments arranged by size: 

                 838     672      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1275    1443    1542 
   Size:       1275     168      99

  Fragments arranged by size: 

                1275     168      99
 
XmnI GAAnn'nnTTC

Cuts at:      0     104    1542 
   Size:        104    1438
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI    BfrBI Bme1580I    BmgBI     BpmI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
   BstAPI    BstXI    BstYI     BtgI   DraIII     EarI     EciI  Eco57MI
 EcoO109I     FalI    Hin4I   HincII     MmeI     MslI   MspA1I     NcoI
     NsiI     NspI    PflMI     PpiI    PpuMI   PspOMI     PstI    SanDI
     SfcI     StyI    TaqII     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AscI    AsiSI     AvaI    AvrII     BaeI     BbeI    BbvCI
     BglI    BglII     BlpI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI     BsmI
    BspEI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI     PfoI     PmeI     PmlI    PshAI     PsiI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI  Tth111I     XbaI     XhoI     XmaI     ZraI
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