Restriction map for pF1KA0749
 (Linear) (Six-base) MAPSORT of: KIAA0749.seq  Check: 5454  from: 1  to: 1971



KIAA0749.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     464    1248    1971 
   Size:        464     784     723

  Fragments arranged by size: 

                 784     723     464
 
AatII G_ACGT'C

Cuts at:      0     680    1971 
   Size:        680    1291
 
AccI GT'mk_AC

Cuts at:      0     261    1971 
   Size:        261    1710
 
AflIII A'CryG_T

Cuts at:      0      46    1287    1971 
   Size:         46    1241     684

  Fragments arranged by size: 

                1241     684      46
 
AhdI GACnn_n'nnGTC

Cuts at:      0     393    1971 
   Size:        393    1578
 
AlwNI CAG_nnn'CTG

Cuts at:      0      20    1049    1173    1708    1971 
   Size:         20    1029     124     535     263

  Fragments arranged by size: 

                1029     535     263     124      20
 
ApaI G_GGCC'C

Cuts at:      0     140     243     413     437    1655    1668    1971 
   Size:        140     103     170      24    1218      13     303

  Fragments arranged by size: 

                1218     303     170     140     103      24      13
 
AscI GG'CGCG_CC

Cuts at:      0    1620    1971 
   Size:       1620     351
 
AvaI C'yCGr_G

Cuts at:      0      60     158     255     290     430     574     682     948 
   Size:         60      98      97      35     140     144     108     266

Cuts at:    948    1782    1816    1971 
   Size:        834      34     155

  Fragments arranged by size: 

                 834     266     155     144     140     108      98      97
                  60      35      34
 
BamHI G'GATC_C

Cuts at:      0      64     657     724    1971 
   Size:         64     593      67    1247

  Fragments arranged by size: 

                1247     593      67      64
 
BanI G'GyrC_C

Cuts at:      0     342     588     862     932    1063    1786    1971 
   Size:        342     246     274      70     131     723     185

  Fragments arranged by size: 

                 723     342     274     246     185     131      70
 
BanII G_rGCy'C

Cuts at:      0      27     140     159     243     253     278     314     413 
   Size:         27     113      19      84      10      25      36      99

Cuts at:    413     437     764     949    1056    1490    1578    1655    1668 
   Size:         24     327     185     107     434      88      77      13

Cuts at:   1668    1739    1971 
   Size:         71     232

  Fragments arranged by size: 

                 434     327     232     185     113     107      99      88
                  84      77      71      36      27      25      24      19
                  13      10
 
BbeI G_GCGC'C

Cuts at:      0     346    1790    1971 
   Size:        346    1444     181

  Fragments arranged by size: 

                1444     346     181
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1858    1971 
   Size:       1858     113
 
BbvCI CC'TCA_GC

Cuts at:      0    1430    1730    1971 
   Size:       1430     300     241

  Fragments arranged by size: 

                1430     300     241
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     756     790    1971 
   Size:        756      34    1181

  Fragments arranged by size: 

                1181     756      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1699    1971 
   Size:       1699     272
 
BglI GCCn_nnn'nGGC

Cuts at:      0      87     304     351    1421    1971 
   Size:         87     217      47    1070     550

  Fragments arranged by size: 

                1070     550     217      87      47
 
BglII A'GATC_T

Cuts at:      0     974    1971 
   Size:        974     997
 
BlpI GC'TnA_GC

Cuts at:      0    1738    1971 
   Size:       1738     233
 
Bme1580I G_kGCm'C

Cuts at:      0     140     243     413     437     593     909    1146    1655 
   Size:        140     103     170      24     156     316     237     509

Cuts at:   1655    1668    1971 
   Size:         13     303

  Fragments arranged by size: 

                 509     316     303     237     170     156     140     103
                  24      13
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      30    1971 
   Size:         30    1941
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1097    1971 
   Size:       1097     874
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     602    1138    1480    1971 
   Size:         84     518     536     342     491

  Fragments arranged by size: 

                 536     518     491     342      84
 
Bpu10I CC'TnA_GC

Cuts at:      0    1430    1730    1971 
   Size:       1430     300     241

  Fragments arranged by size: 

                1430     300     241
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     925    1971 
   Size:        925    1046
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     202     700     718    1105    1971 
   Size:        202     498      18     387     866

  Fragments arranged by size: 

                 866     498     387     202      18
 
BsaAI yAC'GTr

Cuts at:      0      47     519    1211    1971 
   Size:         47     472     692     760

  Fragments arranged by size: 

                 760     692     472      47
 
BsaHI Gr'CG_yC

Cuts at:      0     184     343     492     677    1190    1205    1517    1787 
   Size:        184     159     149     185     513      15     312     270

Cuts at:   1787    1971 
   Size:        184

  Fragments arranged by size: 

                 513     312     270     185     184     184     159     149
                  15
 
BsaWI w'CCGG_w

Cuts at:      0    1066    1107    1971 
   Size:       1066      41     864

  Fragments arranged by size: 

                1066     864      41
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     882     912    1971 
   Size:        882      30    1059

  Fragments arranged by size: 

                1059     882      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1345    1477    1746    1747    1924    1971 
   Size:       1345     132     269       1     177      47

  Fragments arranged by size: 

                1345     269     177     132      47       1
 
BseYI C'CCAG_C

Cuts at:      0     299     497     606    1253    1306    1560    1634    1971 
   Size:        299     198     109     647      53     254      74     337

  Fragments arranged by size: 

                 647     337     299     254     198     109      74      53
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1282    1971 
   Size:       1282     689
 
BsiEI CG_ry'CG

Cuts at:      0     331     508    1629    1971 
   Size:        331     177    1121     342

  Fragments arranged by size: 

                1121     342     331     177
 
BsiHKAI G_wGCw'C

Cuts at:      0     159    1547    1739    1971 
   Size:        159    1388     192     232

  Fragments arranged by size: 

                1388     232     192     159
 
Bsp1286I G_dGCh'C

Cuts at:      0      27     140     159     243     253     278     314     413 
   Size:         27     113      19      84      10      25      36      99

Cuts at:    413     437     593     764     909     949    1056    1146    1490 
   Size:         24     156     171     145      40     107      90     344

Cuts at:   1490    1547    1578    1655    1668    1739    1971 
   Size:         57      31      77      13      71     232

  Fragments arranged by size: 

                 344     232     171     156     145     113     107      99
                  90      84      77      71      57      40      36      31
                  27      25      24      19      13      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     464    1248    1517    1971 
   Size:        464     784     269     454

  Fragments arranged by size: 

                 784     464     454     269
 
BsrBI CCG'CTC

Cuts at:      0     195     742     919    1971 
   Size:        195     547     177    1052

  Fragments arranged by size: 

                1052     547     195     177
 
BsrFI r'CCGG_y

Cuts at:      0      86     503    1624    1971 
   Size:         86     417    1121     347

  Fragments arranged by size: 

                1121     417     347      86
 
BssHII G'CGCG_C

Cuts at:      0    1620    1971 
   Size:       1620     351
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1269    1971 
   Size:       1269     702
 
BstYI r'GATC_y

Cuts at:      0      64     657     665     724     925     974    1883    1971 
   Size:         64     593       8      59     201      49     909      88

  Fragments arranged by size: 

                 909     593     201      88      64      59      49       8
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    1554    1971 
   Size:       1554     417
 
BtgI C'CryG_G

Cuts at:      0    1179    1346    1416    1971 
   Size:       1179     167      70     555

  Fragments arranged by size: 

                1179     555     167      70
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     216     251    1971 
   Size:        216      35    1720

  Fragments arranged by size: 

                1720     216      35
 
EaeI y'GGCC_r

Cuts at:      0     143     328     505     743    1626    1971 
   Size:        143     185     177     238     883     345

  Fragments arranged by size: 

                 883     345     238     185     177     143
 
EagI C'GGCC_G

Cuts at:      0     328     505    1626    1971 
   Size:        328     177    1121     345

  Fragments arranged by size: 

                1121     345     328     177
 
EarI CTCTTCn'nnn_

Cuts at:      0     643    1924    1957    1971 
   Size:        643    1281      33      14

  Fragments arranged by size: 

                1281     643      33      14
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1681    1812    1971 
   Size:       1681     131     159

  Fragments arranged by size: 

                1681     159     131
 
EcoICRI GAG'CTC

Cuts at:      0     157    1737    1971 
   Size:        157    1580     234

  Fragments arranged by size: 

                1580     234     157
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     602    1138    1480    1971 
   Size:         84     518     536     342     491

  Fragments arranged by size: 

                 536     518     491     342      84
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     145    1816    1971 
   Size:        145    1671     155

  Fragments arranged by size: 

                1671     155     145
 
EcoO109I rG'GnC_Cy

Cuts at:      0     136     137     239     409     410     434     541     551 
   Size:        136       1     102     170       1      24     107      10

Cuts at:    551     571    1170    1651    1652    1664    1665    1808    1914 
   Size:         20     599     481       1      12       1     143     106

Cuts at:   1914    1943    1971 
   Size:         29      28

  Fragments arranged by size: 

                 599     481     170     143     136     107     106     102
                  29      28      24      20      12      10       1       1
                   1       1
 
HaeII r_GCGC'y

Cuts at:      0     339     346     361    1790    1971 
   Size:        339       7      15    1429     181

  Fragments arranged by size: 

                1429     339     181      15       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     379     411     642     674     959     991    1971 
   Size:        379      32     231      32     285      32     980

  Fragments arranged by size: 

                 980     379     285     231      32      32      32
 
HincII GTy'rAC

Cuts at:      0     262    1971 
   Size:        262    1709
 
KasI G'GCGC_C

Cuts at:      0     342    1786    1971 
   Size:        342    1444     185

  Fragments arranged by size: 

                1444     342     185
 
MluI A'CGCG_T

Cuts at:      0    1287    1971 
   Size:       1287     684
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     723    1105    1264    1577    1803    1971 
   Size:        723     382     159     313     226     168

  Fragments arranged by size: 

                 723     382     313     226     168     159
 
MspA1I CmG'CkG

Cuts at:      0     303     497     610    1418    1617    1858    1971 
   Size:        303     194     113     808     199     241     113

  Fragments arranged by size: 

                 808     303     241     199     194     113     113
 
NaeI GCC'GGC

Cuts at:      0      88    1626    1971 
   Size:         88    1538     345

  Fragments arranged by size: 

                1538     345      88
 
NarI GG'CG_CC

Cuts at:      0     343    1787    1971 
   Size:        343    1444     184

  Fragments arranged by size: 

                1444     343     184
 
NgoMIV G'CCGG_C

Cuts at:      0      86    1624    1971 
   Size:         86    1538     347

  Fragments arranged by size: 

                1538     347      86
 
PasI CC'CwG_GG

Cuts at:      0      92     391     413    1049    1167    1578    1648    1971 
   Size:         92     299      22     636     118     411      70     323

  Fragments arranged by size: 

                 636     411     323     299     118      92      70      22
            
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      20     736     992    1049    1971 
   Size:         20     716     256      57     922

  Fragments arranged by size: 

                 922     716     256      57      20
 
PfoI T'CCnGG_A

Cuts at:      0    1056    1971 
   Size:       1056     915
 
PmlI CAC'GTG

Cuts at:      0      47    1211    1971 
   Size:         47    1164     760

  Fragments arranged by size: 

                1164     760      47
 
PpuMI rG'GwC_Cy

Cuts at:      0     551    1808    1914    1943    1971 
   Size:        551    1257     106      29      28

  Fragments arranged by size: 

                1257     551     106      29      28
 
PspOMI G'GGCC_C

Cuts at:      0     136     239     409     433    1651    1664    1971 
   Size:        136     103     170      24    1218      13     307

  Fragments arranged by size: 

                1218     307     170     136     103      24      13
 
PspXI vC'TCGA_Gb

Cuts at:      0     948    1971 
   Size:        948    1023
 
PstI C_TGCA'G

Cuts at:      0     113    1971 
   Size:        113    1858
 
PvuII CAG'CTG

Cuts at:      0     303     610    1971 
   Size:        303     307    1361

  Fragments arranged by size: 

                1361     307     303
 
SacI G_AGCT'C

Cuts at:      0     159    1739    1971 
   Size:        159    1580     232

  Fragments arranged by size: 

                1580     232     159
 
SacII CC_GC'GG

Cuts at:      0    1419    1971 
   Size:       1419     552
 
SalI G'TCGA_C

Cuts at:      0     260    1971 
   Size:        260    1711
 
SanDI GG'GwC_CC

Cuts at:      0    1943    1971 
   Size:       1943      28
 
SfcI C'TryA_G

Cuts at:      0     109     848    1971 
   Size:        109     739    1123

  Fragments arranged by size: 

                1123     739     109
 
SfoI GGC'GCC

Cuts at:      0     344    1788    1971 
   Size:        344    1444     183

  Fragments arranged by size: 

                1444     344     183
 
SmaI CCC'GGG

Cuts at:      0      62     257     432     576    1784    1971 
   Size:         62     195     175     144    1208     187

  Fragments arranged by size: 

                1208     195     187     175     144      62
 
SmlI C'TyrA_G

Cuts at:      0     940     948    1971 
   Size:        940       8    1023

  Fragments arranged by size: 

                1023     940       8
 
SrfI GCCC'GGGC

Cuts at:      0     432    1971 
   Size:        432    1539
 
StyI C'CwwG_G

Cuts at:      0     203     554    1971 
   Size:        203     351    1417

  Fragments arranged by size: 

                1417     351     203
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     226    1971 
   Size:        226    1745
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1338    1370    1971 
   Size:       1338      32     601

  Fragments arranged by size: 

                1338     601      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1873    1971 
   Size:       1873      98
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     398     494    1971 
   Size:        398      96    1477

  Fragments arranged by size: 

                1477     398      96
 
XhoI C'TCGA_G

Cuts at:      0     948    1971 
   Size:        948    1023
 
XmaI C'CCGG_G

Cuts at:      0      60     255     430     574    1782    1971 
   Size:         60     195     175     144    1208     189

  Fragments arranged by size: 

                1208     195     189     175     144      60
 
XmnI GAAnn'nnTTC

Cuts at:      0    1830    1971 
   Size:       1830     141
 
ZraI GAC'GTC

Cuts at:      0     678    1971 
   Size:        678    1293
 

 Enzymes that do cut:

     AarI    AatII     AccI   AflIII     AhdI    AlwNI     ApaI     AscI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI
   BssHII   BstAPI    BstYI   Bsu36I     BtgI    CspCI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I
   HincII     KasI     MluI     MmeI   MspA1I     NaeI     NarI   NgoMIV
     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PstI
    PvuII     SacI    SacII     SalI    SanDI     SfcI     SfoI     SmaI
     SmlI     SrfI     StyI    TaqII     TstI  Tth111I     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AleI     AlfI
     AloI    ApaLI     ApoI     AseI    AsiSI    AvrII     BaeI     BclI
    BfrBI    BmgBI     BmtI    BsaBI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrDI    BsrGI    BssSI    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI
     MscI     MslI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI     PmeI     PpiI    PshAI     PsiI     PsrI
     PvuI    RsrII     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SspI     StuI     SwaI     TatI
     XbaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com