Restriction map for
pF1KB8579
(Circular) (Six-base) MAPSORT of: pF1KB8579.seq Check: 2929 from: 1 to: 3809
pF1KB8579 3809 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AatII G_ACGT'C
Cuts at: 554 554
Size: 3809
AccI GT'mk_AC
Cuts at: 847 847
Size: 3809
Acc65I G'GTAC_C
Cuts at: 825 825
Size: 3809
AclI AA'CG_TT
Cuts at: 3649 3649
Size: 3809
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 702 1634 2066 2813 3081 702
Size: 932 432 747 268 1430
Fragments arranged by size:
1430 932 747 432 268
AflIII A'CryG_T
Cuts at: 1161 2265 3242 1161
Size: 1104 977 1728
Fragments arranged by size:
1728 1104 977
AgeI A'CCGG_T
Cuts at: 2197 2197
Size: 3809
AhdI GACnn_n'nnGTC
Cuts at: 270 270
Size: 3809
AleI CACnn'nnGTG
Cuts at: 383 383
Size: 3809
AlwNI CAG_nnn'CTG
Cuts at: 405 681 729 1114 2681 405
Size: 276 48 385 1567 1533
Fragments arranged by size:
1567 1533 385 276 48
ApaLI G'TGCA_C
Cuts at: 2579 2579
Size: 3809
ApoI r'AATT_y
Cuts at: 813 3162 3418 813
Size: 2349 256 1204
Fragments arranged by size:
2349 1204 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3809
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3809
AvaI C'yCGr_G
Cuts at: 221 829 221
Size: 608 3201
BamHI G'GATC_C
Cuts at: 834 834
Size: 3809
BanI G'GyrC_C
Cuts at: 615 825 1239 1487 1522 615
Size: 210 414 248 35 2902
Fragments arranged by size:
2902 414 248 210 35
BanII G_rGCy'C
Cuts at: 262 439 823 1853 262
Size: 177 384 1030 2218
Fragments arranged by size:
2218 1030 384 177
BbeI G_GCGC'C
Cuts at: 1243 1491 1243
Size: 248 3561
BbsI GAAGACnn'nnnn_
Cuts at: 317 317
Size: 3809
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3775
BciVI GTATCCnnnnn_n'
Cuts at: 1707 2468 3809 1707
Size: 761 1341 1707
Fragments arranged by size:
1707 1341 761
BclI T'GATC_A
Cuts at: 1329 1329
Size: 3809
BglI GCCn_nnn'nGGC
Cuts at: 488 488
Size: 3809
BglII A'GATC_T
Cuts at: 1324 3361 1324
Size: 2037 1772
BlpI GC'TnA_GC
Cuts at: 201 736 921 201
Size: 535 185 3089
Fragments arranged by size:
3089 535 185
Bme1580I G_kGCm'C
Cuts at: 620 1434 1527 2583 620
Size: 814 93 1056 1846
Fragments arranged by size:
1846 1056 814 93
BmrI ACTGGGnnnn_n'
Cuts at: 1021 1102 1437 3581 1021
Size: 81 335 2144 1249
Fragments arranged by size:
2144 1249 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 3809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 274
Size: 3809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 985 1864 2356 2654 985
Size: 879 492 298 2140
Fragments arranged by size:
2140 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3513 3513
Size: 3809
BsaAI yAC'GTr
Cuts at: 1162 1162
Size: 3809
BsaBI GATnn'nnATC
Cuts at: 6 1323 3360 6
Size: 1317 2037 455
Fragments arranged by size:
2037 1317 455
BsaHI Gr'CG_yC
Cuts at: 444 551 1240 1488 2190 3686 444
Size: 107 689 248 702 1496 567
Fragments arranged by size:
1496 702 689 567 248 107
BsaWI w'CCGG_w
Cuts at: 998 1519 2197 2471 2618 3029 998
Size: 521 678 274 147 411 1778
Fragments arranged by size:
1778 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 217 247 217
Size: 30 3779
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 138 241 811 138
Size: 103 570 3136
Fragments arranged by size:
3136 570 103
BseYI C'CCAG_C
Cuts at: 432 607 1234 1992 2569 3065 432
Size: 175 627 758 577 496 1176
Fragments arranged by size:
1176 758 627 577 496 175
BsiEI CG_ry'CG
Cuts at: 104 193 1351 1397 2605 104
Size: 89 1158 46 1208 1308
Fragments arranged by size:
1308 1208 1158 89 46
BsiHKAI G_wGCw'C
Cuts at: 262 742 823 1601 1791 2583 262
Size: 480 81 778 190 792 1488
Fragments arranged by size:
1488 792 778 480 190 81
Bsp1286I G_dGCh'C
Cuts at: 262 439 620 742 823 1434 1527 1601 1791
Size: 177 181 122 81 611 93 74 190
Cuts at: 1791 1853 2583 262
Size: 62 730 1488
Fragments arranged by size:
1488 730 611 190 181 177 122 93
81 74 62
BspEI T'CCGG_A
Cuts at: 3029 3029
Size: 3809
BspHI T'CATG_A
Cuts at: 2985 2985
Size: 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 859 1073 1375 1756 859
Size: 214 302 381 2912
Fragments arranged by size:
2912 381 302 214
BsrBI CCG'CTC
Cuts at: 50 242 2101 2155 3639 3804 50
Size: 192 1859 54 1484 165 55
Fragments arranged by size:
1859 1484 192 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1721 1721
Size: 3809
BsrFI r'CCGG_y
Cuts at: 1807 1988 2197 1807
Size: 181 209 3419
Fragments arranged by size:
3419 209 181
BsrGI T'GTAC_A
Cuts at: 770 770
Size: 3809
BssHII G'CGCG_C
Cuts at: 451 451
Size: 3809
BssSI C'ACGA_G
Cuts at: 246 637 2080 2438 246
Size: 391 1443 358 1617
Fragments arranged by size:
1617 1443 391 358
BstAPI GCAn_nnn'nTGC
Cuts at: 405 575 1186 405
Size: 170 611 3028
Fragments arranged by size:
3028 611 170
BstBI TT'CG_AA
Cuts at: 2170 2170
Size: 3809
BstXI CCAn_nnnn'nTGG
Cuts at: 252 252
Size: 3809
BstYI r'GATC_y
Cuts at: 1 671 834 1301 1324 1659 1905 2917 3003
Size: 670 163 467 23 335 246 1012 86
Cuts at: 3003 3015 3361 1
Size: 12 346 449
Fragments arranged by size:
1012 670 467 449 346 335 246 163
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 368 368
Size: 3809
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1759 1940 2103 1759
Size: 181 163 3465
Fragments arranged by size:
3465 181 163
BtsI GCAGTG_nn'
Cuts at: 1052 1052
Size: 3809
DraI TTT'AAA
Cuts at: 808 808
Size: 3809
DraIII CAC_nnn'GTG
Cuts at: 585 585
Size: 3809
DrdI GACnn_nn'nnGTC
Cuts at: 1515 2373 1515
Size: 858 2951
EaeI y'GGCC_r
Cuts at: 190 1394 1568 1959 1986 3331 190
Size: 1204 174 391 27 1345 668
Fragments arranged by size:
1345 1204 668 391 174 27
EagI C'GGCC_G
Cuts at: 190 1394 190
Size: 1204 2605
EarI CTCTTCn'nnn_
Cuts at: 156 1832 2042 156
Size: 1676 210 1923
Fragments arranged by size:
1923 1676 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 681 2017 2327 2473 3619 681
Size: 1336 310 146 1146 871
Fragments arranged by size:
1336 1146 871 310 146
EcoICRI GAG'CTC
Cuts at: 260 821 260
Size: 561 3248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 702 1634 2066 2813 3081 274
Size: 428 932 432 747 268 1002
Fragments arranged by size:
1002 932 747 432 428 268
EcoNI CCTnn'n_nnAGG
Cuts at: 421 421
Size: 3809
EcoO109I rG'GnC_Cy
Cuts at: 948 948
Size: 3809
EcoRI G'AATT_C
Cuts at: 813 813
Size: 3809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1828 1828
Size: 3809
FspI TGC'GCA
Cuts at: 1590 1590
Size: 3809
HaeII r_GCGC'y
Cuts at: 544 1243 1491 2513 3492 544
Size: 699 248 1022 979 861
Fragments arranged by size:
1022 979 861 699 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 115 147 256 288 319 351 1651 1681 1683
Size: 32 109 32 31 32 1300 30 2
Cuts at: 1683 1713 115
Size: 30 2211
Fragments arranged by size:
2211 1300 109 32 32 32 31 30
30 2
HincII GTy'rAC
Cuts at: 848 848
Size: 3809
KasI G'GCGC_C
Cuts at: 1239 1487 1239
Size: 248 3561
KpnI G_GTAC'C
Cuts at: 829 829
Size: 3809
MluI A'CGCG_T
Cuts at: 3242 3242
Size: 3809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2480 2664 2480
Size: 184 3625
MscI TGG'CCA
Cuts at: 1570 1570
Size: 3809
MslI CAynn'nnrTG
Cuts at: 383 1798 1925 383
Size: 1415 127 2267
Fragments arranged by size:
2267 1415 127
MspA1I CmG'CkG
Cuts at: 402 594 630 786 920 1234 1594 2607 2852
Size: 192 36 156 134 314 360 1013 245
Cuts at: 2852 402
Size: 1359
Fragments arranged by size:
1359 1013 360 314 245 192 156 134
36
NaeI GCC'GGC
Cuts at: 1990 1990
Size: 3809
NarI GG'CG_CC
Cuts at: 1240 1488 1240
Size: 248 3561
NgoMIV G'CCGG_C
Cuts at: 1988 1988
Size: 3809
NspI r_CATG'y
Cuts at: 862 1362 2269 862
Size: 500 907 2402
Fragments arranged by size:
2402 907 500
PciI A'CATG_T
Cuts at: 2265 2265
Size: 3809
PflMI CCAn_nnn'nTGG
Cuts at: 585 585
Size: 3809
PmeI GTTT'AAAC
Cuts at: 808 808
Size: 3809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2948 2980 2948
Size: 32 3777
PstI C_TGCA'G
Cuts at: 410 856 1541 410
Size: 446 685 2678
Fragments arranged by size:
2678 685 446
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3809
PvuII CAG'CTG
Cuts at: 402 594 630 786 1234 1594 402
Size: 192 36 156 448 360 2617
Fragments arranged by size:
2617 448 360 192 156 36
RsrII CG'GwC_CG
Cuts at: 2004 2004
Size: 3809
SacI G_AGCT'C
Cuts at: 262 823 262
Size: 561 3248
SalI G'TCGA_C
Cuts at: 846 846
Size: 3809
SapI GCTCTTCn'nnn_
Cuts at: 1832 2042 1832
Size: 210 3599
SbfI CC_TGCA'GG
Cuts at: 856 856
Size: 3809
ScaI AGT'ACT
Cuts at: 3326 3326
Size: 3809
SfcI C'TryA_G
Cuts at: 33 406 513 852 1537 2530 2721 33
Size: 373 107 339 685 993 191 1121
Fragments arranged by size:
1121 993 685 373 339 191 107
SfoI GGC'GCC
Cuts at: 1241 1489 1241
Size: 248 3561
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3809
SmaI CCC'GGG
Cuts at: 223 831 223
Size: 608 3201
SmlI C'TyrA_G
Cuts at: 964 1879 2371 2633 964
Size: 915 492 262 2140
Fragments arranged by size:
2140 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3809
SphI G_CATG'C
Cuts at: 862 1362 862
Size: 500 3309
StyI C'CwwG_G
Cuts at: 943 1297 943
Size: 354 3455
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1392 1525 1984 2193 1392
Size: 133 459 209 3008
Fragments arranged by size:
3008 459 209 133
TatI w'GTAC_w
Cuts at: 770 3324 770
Size: 2554 1255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 3777
Tth111I GACn'n_nGTC
Cuts at: 1606 1606
Size: 3809
XbaI T'CTAG_A
Cuts at: 840 840
Size: 3809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1304 1304
Size: 3809
XmaI C'CCGG_G
Cuts at: 221 829 221
Size: 608 3201
ZraI GAC'GTC
Cuts at: 552 552
Size: 3809
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AlfI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsgI BsiWI
BsmI BsmBI BstEII BstZ17I BtgI ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI