Restriction map for pF1KE0508
 (Linear) (Six-base) MAPSORT of: KIEE0508.seq  Check: 8689  from: 1  to: 504



KIEE0508.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     315     419     504 
   Size:        315     104      85

  Fragments arranged by size: 

                 315     104      85
 
AflIII A'CryG_T

Cuts at:      0      35     504 
   Size:         35     469
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     268     300     504 
   Size:        268      32     204

  Fragments arranged by size: 

                 268     204      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     208     354     504 
   Size:        208     146     150

  Fragments arranged by size: 

                 208     150     146
 
ApaLI G'TGCA_C

Cuts at:      0     127     504 
   Size:        127     377
 
AvaI C'yCGr_G

Cuts at:      0     177     504 
   Size:        177     327
 
BanII G_rGCy'C

Cuts at:      0     478     504 
   Size:        478      26
 
BlpI GC'TnA_GC

Cuts at:      0      70     504 
   Size:         70     434
 
Bme1580I G_kGCm'C

Cuts at:      0     131     504 
   Size:        131     373
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     368     504 
   Size:        368     136
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     279     504 
   Size:        279     225
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     185     274     449     504 
   Size:        150      35      89     175      55

  Fragments arranged by size: 

                 175     150      89      55      35
 
BsaAI yAC'GTr

Cuts at:      0      36     504 
   Size:         36     468
 
BsaWI w'CCGG_w

Cuts at:      0      21     504 
   Size:         21     483
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     250     268     280     298     504 
   Size:        250      18      12      18     206

  Fragments arranged by size: 

                 250     206      18      18      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     255     504 
   Size:        255     249
 
BseYI C'CCAG_C

Cuts at:      0      84     504 
   Size:         84     420
 
BsiHKAI G_wGCw'C

Cuts at:      0     131     478     504 
   Size:        131     347      26

  Fragments arranged by size: 

                 347     131      26
 
Bsp1286I G_dGCh'C

Cuts at:      0     131     478     504 
   Size:        131     347      26

  Fragments arranged by size: 

                 347     131      26
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     398     504 
   Size:        398     106
 
BsrDI GCAATG_nn'

Cuts at:      0     187     504 
   Size:        187     317
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      57      84     504 
   Size:         57      27     420

  Fragments arranged by size: 

                 420      57      27
 
BstYI r'GATC_y

Cuts at:      0     455     504 
   Size:        455      49
 
BtgI C'CryG_G

Cuts at:      0     479     504 
   Size:        479      25
 
BtsI GCAGTG_nn'

Cuts at:      0     159     504 
   Size:        159     345
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     399     434     504 
   Size:        399      35      70

  Fragments arranged by size: 

                 399      70      35
 
EaeI y'GGCC_r

Cuts at:      0     495     504 
   Size:        495       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     378     504 
   Size:        378     126
 
EcoICRI GAG'CTC

Cuts at:      0     476     504 
   Size:        476      28
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     185     274     315     419     449     504 
   Size:        150      35      89      41     104      30      55

  Fragments arranged by size: 

                 150     104      89      55      41      35      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     467     504 
   Size:        467      37
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     379     411     447     479     504 
   Size:        379      32      36      32      25

  Fragments arranged by size: 

                 379      36      32      32      25
 
MfeI C'AATT_G

Cuts at:      0     318     504 
   Size:        318     186
 
MslI CAynn'nnrTG

Cuts at:      0     124     134     504 
   Size:        124      10     370

  Fragments arranged by size: 

                 370     124      10
 
MspA1I CmG'CkG

Cuts at:      0     170     504 
   Size:        170     334
 
PasI CC'CwG_GG

Cuts at:      0     270     504 
   Size:        270     234
 
PfoI T'CCnGG_A

Cuts at:      0     440     465     504 
   Size:        440      25      39

  Fragments arranged by size: 

                 440      39      25
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     268     300     504 
   Size:        268      32     204

  Fragments arranged by size: 

                 268     204      32
 
PvuII CAG'CTG

Cuts at:      0     170     504 
   Size:        170     334
 
SacI G_AGCT'C

Cuts at:      0     478     504 
   Size:        478      26
 
SmaI CCC'GGG

Cuts at:      0     179     504 
   Size:        179     325
 
StuI AGG'CCT

Cuts at:      0      56     298     504 
   Size:         56     242     206

  Fragments arranged by size: 

                 242     206      56
 
TatI w'GTAC_w

Cuts at:      0     226     504 
   Size:        226     278
 
XmaI C'CCGG_G

Cuts at:      0     177     504 
   Size:        177     327
 
XmnI GAAnn'nnTTC

Cuts at:      0     248     504 
   Size:        248     256
 

 Enzymes that do cut:

     AcuI   AflIII     AloI    AlwNI    ApaLI     AvaI    BanII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BsaAI    BsaWI    BsaXI    BseRI
    BseYI  BsiHKAI Bsp1286I    BspMI    BsrDI    BstXI    BstYI     BtgI
     BtsI    CspCI     EaeI     EciI  EcoICRI  Eco57MI    EcoNI    Hin4I
     MfeI     MslI   MspA1I     PasI     PfoI     PpiI    PvuII     SacI
     SmaI     StuI     TatI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     ApaI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmtI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI     DraI
   DraIII     DrdI     EagI     EarI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI
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