Restriction map for pF1KE0377
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwNI       ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvrII       
BbsI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmI        BspEI       
BspLU11I    BsrBI       Bst1107I    BstAPI      BstBI       
BstZ17I     CelII       CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco52I      
EcoRI       EcoT22I     FbaI        FseI        HpaI        
KpnI        KspI        MaeI        MamI        MfeI        
MluI        MroI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        Sse8387I    SspI        SwaI        
TfiI        Tth111I     Van91I      XbaI        XcmI        
XhoI        


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# ONE CUT ENZYMES (   59 enzymes) #
###################################

Aor51HI       188  AGCGCT               AGC^GCT
ApaI          220  GGGCCC               GGGCC^C
Asp700I       888  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1223  CCCGGG               C^[CT]CG[GA]G
AviII         605  TGCGCA               TGC^GCA
BalI         1331  TGGCCA               TGG^CCA
BamHI         777  GGATCC               G^GATCC
BanI          493  GGCGCC               G^G[CT][GA]CC
BbeI          493  GGCGCC               GGCGC^C
BbrPI        1429  CACGTG               CAC^GTG
BbvCI         283  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1371  GCAGAACTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI          730  CTGGAG               CTGGAG(16/14)
BsaAI        1429  CACGTG               [CT]AC^GT[GA]
BsoBI        1223  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      161  TGTACA               T^GTACA
BspDI         502  ATCGAT               AT^CGAT
BsrDI         116  CATTGC               GCAATG(2/0)
BsrFI        1013  ACCGGC               [GA]^CCGG[CT]
BsrGI         161  TGTACA               T^GTACA
BssHII        366  GCGCGC               G^CGCGC
BssSI        1069  CACGAG               CACGAG(-5/-1)
BstEII         24  GGTGACC              G^GT[ACGT]ACC
BstPI          24  GGTGACC              G^GT[ACGT]ACC
Cfr10I       1013  ACCGGC               [GA]^CCGG[CT]
ClaI          502  ATCGAT               AT^CGAT
EaeI         1331  TGGCCA               [CT]^GGCC[GA]
Eco47III      188  AGCGCT               AGC^GCT
EcoNI        1152  CCTCCCTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I        24  GGTGACC              G^GT[ACGT]ACC
EcoRV        1408  GATATC               GAT^ATC
FspI          605  TGCGCA               TGC^GCA
HgaI          472  GACGC                GACGC(5/10)
HincII       1120  GTCAAC               GT[CT]^[GA]AC
HindII       1120  GTCAAC               GT[CT]^[GA]AC
HindIII       621  AAGCTT               A^AGCTT
HinfI         805  GACTC                G^A[ACGT]TC
KasI          493  GGCGCC               G^GCGCC
MluNI        1331  TGGCCA               TGG^CCA
MscI         1331  TGGCCA               TGG^CCA
NarI          493  GGCGCC               GG^CGCC
NcoI         1100  CCATGG               C^CATGG
PleI          805  GACTC                GAGTC(4/5)
PmaCI        1429  CACGTG               CAC^GTG
PmlI         1429  CACGTG               CAC^GTG
PpuMI         105  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          139  CTGCAG               CTGCA^G
PvuII         142  CAGCTG               CAG^CTG
SacI         1342  GAGCTC               GAGCT^C
SexAI         869  ACCTGGT              A^CC[AT]GGT
SfcI          139  CTGCAG               C^T[GA][CT]AG
SfoI          493  GGCGCC               GGC^GCC
SmaI         1223  CCCGGG               CCC^GGG
SphI          821  GCATGC               GCATG^C
SspBI         161  TGTACA               T^GTACA
Tsp509I       784  AATT                 ^AATT
XmaCI        1223  CCCGGG               C^CCGGG
XmaI         1223  CCCGGG               C^CCGGG
XmnI          888  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccII         367  CGCG                 CG^CG
              427  CGCG                
AcyI          472  GACGCC               G[GA]^CG[CT]C
              493  GGCGCC              
AfaI          162  GTAC                 GT^AC
             1311  GTAC                
AflIII       1415  ACGTGT               A^C[GA][CT]GT
             1428  ACACGT              
BsaHI         472  GACGCC               G[GA]^CG[CT]C
              493  GGCGCC              
BsaI          910  GAGACC               GGTCTC(1/5)
             1199  GAGACC              
BspHI         173  TCATGA               T^CATGA
              950  TCATGA              
BspMI         137  ACCTGC               ACCTGC(4/8)
              686  ACCTGC              
BsrI          323  ACTGG                ACTGG(1/-1)
              627  CCAGT               
BstF5I        444  CATCC                GGATG(2/0)
              816  CATCC               
BstUI         367  CGCG                 CG^CG
              427  CGCG                
BstYI         777  GGATCC               [GA]^GATC[CT]
             1073  AGATCC              
EcoT14I       974  CCAAGG               C^C[AT][AT]GG
             1100  CCATGG              
FokI          444  CATCC                GGATG(9/13)
              816  CATCC               
Hin1I         472  GACGCC               G[GA]^CG[CT]C
              493  GGCGCC              
MaeII        1415  ACGT                 A^CGT
             1430  ACGT                
MaeIII         25  GTGAC                ^GT[ACGT]AC
              597  GTCAC               
MflI          777  GGATCC               [GA]^GATC[CT]
             1073  AGATCC              
MseI          713  TTAA                 T^TAA
             1268  TTAA                
MspA1I        142  CAGCTG               C[AC]G^C[GT]G
              294  CAGCGG              
MvnI          367  CGCG                 CG^CG
              427  CGCG                
NspI          821  GCATGC               [GA]CATG^[CT]
             1091  ACATGC              
RcaI          173  TCATGA               T^CATGA
              950  TCATGA              
RsaI          162  GTAC                 GT^AC
             1311  GTAC                
SapI           81  GAAGAGC              GCTCTTC(1/4)
              327  GCTCTTC             
StuI          707  AGGCCT               AGG^CCT
             1326  AGGCCT              
StyI          974  CCAAGG               C^C[AT][AT]GG
             1100  CCATGG              
Tru9I         713  TTAA                 T^TAA
             1268  TTAA                
Tsp45I         25  GTGAC                ^GT[GC]AC
              597  GTCAC               
XhoII         777  GGATCC               [GA]^GATC[CT]
             1073  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AluI        AlwI        AocI        AspHI       
AvaII       BanII       BbvI        BcnI        BmyI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BssKI       BstNI       
BstXI       Bsu36I      Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EarI        
Eco81I      EcoO109I    EcoRII      FauI        Fnu4HI      
HaeII       HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        Ksp632I     MboI        
MboII       MnlI        MslI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       TaqI        
TseI        TspRI       TthHB8I     
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