Restriction map for pF1KE0377
 (Linear) (Six-base) MAPSORT of: KIEE0377.seq  Check: 3425  from: 1  to: 1482



KIEE0377.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     695    1482 
   Size:        695     787
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1187    1482 
   Size:       1187     295
 
AfeI AGC'GCT

Cuts at:      0     190    1482 
   Size:        190    1292
 
AflIII A'CryG_T

Cuts at:      0    1415    1428    1482 
   Size:       1415      13      54

  Fragments arranged by size: 

                1415      54      13
 
AleI CACnn'nnGTG

Cuts at:      0    1168    1482 
   Size:       1168     314
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     943    1482 
   Size:        943     539
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     574     606    1482 
   Size:        574      32     876

  Fragments arranged by size: 

                 876     574      32
 
ApaI G_GGCC'C

Cuts at:      0     224    1482 
   Size:        224    1258
 
AvaI C'yCGr_G

Cuts at:      0    1223    1482 
   Size:       1223     259
 
BamHI G'GATC_C

Cuts at:      0     777    1482 
   Size:        777     705
 
BanI G'GyrC_C

Cuts at:      0     493    1482 
   Size:        493     989
 
BanII G_rGCy'C

Cuts at:      0     224    1189    1346    1482 
   Size:        224     965     157     136

  Fragments arranged by size: 

                 965     224     157     136
 
BbeI G_GCGC'C

Cuts at:      0     497    1482 
   Size:        497     985
 
BbvCI CC'TCA_GC

Cuts at:      0     284    1482 
   Size:        284    1198
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1360    1394    1482 
   Size:       1360      34      88

  Fragments arranged by size: 

                1360      88      34
 
Bme1580I G_kGCm'C

Cuts at:      0     224     487     907    1165    1292    1482 
   Size:        224     263     420     258     127     190

  Fragments arranged by size: 

                 420     263     258     224     190     127
 
BmgBI CAC'GTC

Cuts at:      0    1416    1482 
   Size:       1416      66
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     684    1111    1482 
   Size:        684     427     371

  Fragments arranged by size: 

                 684     427     371
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     751    1482 
   Size:        751     731
 
Bpu10I CC'TnA_GC

Cuts at:      0     284     520    1482 
   Size:        284     236     962

  Fragments arranged by size: 

                 962     284     236
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     904    1193    1482 
   Size:        904     289     289

  Fragments arranged by size: 

                 904     289     289
 
BsaAI yAC'GTr

Cuts at:      0    1431    1482 
   Size:       1431      51
 
BsaHI Gr'CG_yC

Cuts at:      0     473     494    1482 
   Size:        473      21     988

  Fragments arranged by size: 

                 988     473      21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     301     466     844     847    1000    1482 
   Size:        301     165     378       3     153     482

  Fragments arranged by size: 

                 482     378     301     165     153       3
 
BseYI C'CCAG_C

Cuts at:      0     615    1182    1238    1482 
   Size:        615     567      56     244

  Fragments arranged by size: 

                 615     567     244      56
 
BsiHKAI G_wGCw'C

Cuts at:      0     761     922    1346    1482 
   Size:        761     161     424     136

  Fragments arranged by size: 

                 761     424     161     136
 
Bsp1286I G_dGCh'C

Cuts at:      0     224     487     761     907     922    1165    1189    1292 
   Size:        224     263     274     146      15     243      24     103

Cuts at:   1292    1346    1482 
   Size:         54     136

  Fragments arranged by size: 

                 274     263     243     224     146     136     103      54
                  24      15
 
BspHI T'CATG_A

Cuts at:      0     173     950    1482 
   Size:        173     777     532

  Fragments arranged by size: 

                 777     532     173
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     146     695    1482 
   Size:        146     549     787

  Fragments arranged by size: 

                 787     549     146
 
BsrDI GCAATG_nn'

Cuts at:      0     115    1482 
   Size:        115    1367
 
BsrFI r'CCGG_y

Cuts at:      0    1013    1482 
   Size:       1013     469
 
BsrGI T'GTAC_A

Cuts at:      0     161    1482 
   Size:        161    1321
 
BssHII G'CGCG_C

Cuts at:      0     366    1482 
   Size:        366    1116
 
BssSI C'ACGA_G

Cuts at:      0    1069    1482 
   Size:       1069     413
 
BstEII G'GTnAC_C

Cuts at:      0      24    1482 
   Size:         24    1458
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     216     753    1084    1107    1431    1482 
   Size:        216     537     331      23     324      51

  Fragments arranged by size: 

                 537     331     324     216      51      23
 
BstYI r'GATC_y

Cuts at:      0     777    1073    1482 
   Size:        777     296     409

  Fragments arranged by size: 

                 777     409     296
 
Bsu36I CC'TnA_GG

Cuts at:      0       9     403    1157    1403    1482 
   Size:          9     394     754     246      79

  Fragments arranged by size: 

                 754     394     246      79       9
 
BtgI C'CryG_G

Cuts at:      0     644     790    1100    1482 
   Size:        644     146     310     382

  Fragments arranged by size: 

                 644     382     310     146
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     378     726    1482 
   Size:        378     348     756

  Fragments arranged by size: 

                 756     378     348
 
BtsI GCAGTG_nn'

Cuts at:      0    1067    1482 
   Size:       1067     415
 
ClaI AT'CG_AT

Cuts at:      0     503    1482 
   Size:        503     979
 
EaeI y'GGCC_r

Cuts at:      0    1331    1482 
   Size:       1331     151
 
EarI CTCTTCn'nnn_

Cuts at:      0      76     334     675     898    1267    1357    1482 
   Size:         76     258     341     223     369      90     125

  Fragments arranged by size: 

                 369     341     258     223     125      90      76
 
EcoICRI GAG'CTC

Cuts at:      0    1344    1482 
   Size:       1344     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     751    1187    1482 
   Size:        751     436     295

  Fragments arranged by size: 

                 751     436     295
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1156    1482 
   Size:       1156     326
 
EcoO109I rG'GnC_Cy

Cuts at:      0     106     220     221    1482 
   Size:        106     114       1    1261

  Fragments arranged by size: 

                1261     114     106       1
 
EcoRV GAT'ATC

Cuts at:      0    1410    1482 
   Size:       1410      72
 
FspI TGC'GCA

Cuts at:      0     607    1482 
   Size:        607     875
 
HaeII r_GCGC'y

Cuts at:      0     192     387     497    1482 
   Size:        192     195     110     985

  Fragments arranged by size: 

                 985     195     192     110
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     580     612    1482 
   Size:        580      32     870

  Fragments arranged by size: 

                 870     580      32
 
HincII GTy'rAC

Cuts at:      0    1122    1482 
   Size:       1122     360
 
HindIII A'AGCT_T

Cuts at:      0     621    1482 
   Size:        621     861
 
KasI G'GCGC_C

Cuts at:      0     493    1482 
   Size:        493     989
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1241    1482 
   Size:       1241     241
 
MscI TGG'CCA

Cuts at:      0    1333    1482 
   Size:       1333     149
 
MslI CAynn'nnrTG

Cuts at:      0     820    1168    1366    1482 
   Size:        820     348     198     116

  Fragments arranged by size: 

                 820     348     198     116
 
MspA1I CmG'CkG

Cuts at:      0     144     296    1482 
   Size:        144     152    1186

  Fragments arranged by size: 

                1186     152     144
 
NarI GG'CG_CC

Cuts at:      0     494    1482 
   Size:        494     988
 
NcoI C'CATG_G

Cuts at:      0    1100    1482 
   Size:       1100     382
 
NspI r_CATG'y

Cuts at:      0     825    1095    1482 
   Size:        825     270     387

  Fragments arranged by size: 

                 825     387     270
 
PfoI T'CCnGG_A

Cuts at:      0     102     446    1247    1482 
   Size:        102     344     801     235

  Fragments arranged by size: 

                 801     344     235     102
 
PmlI CAC'GTG

Cuts at:      0    1431    1482 
   Size:       1431      51
 
PpuMI rG'GwC_Cy

Cuts at:      0     106    1482 
   Size:        106    1376
 
PspOMI G'GGCC_C

Cuts at:      0     220    1482 
   Size:        220    1262
 
PstI C_TGCA'G

Cuts at:      0     143    1482 
   Size:        143    1339
 
PvuII CAG'CTG

Cuts at:      0     144    1482 
   Size:        144    1338
 
SacI G_AGCT'C

Cuts at:      0    1346    1482 
   Size:       1346     136
 
SanDI GG'GwC_CC

Cuts at:      0     106    1482 
   Size:        106    1376
 
SapI GCTCTTCn'nnn_

Cuts at:      0      76     334    1482 
   Size:         76     258    1148

  Fragments arranged by size: 

                1148     258      76
 
SexAI A'CCwGG_T

Cuts at:      0     869    1482 
   Size:        869     613
 
SfcI C'TryA_G

Cuts at:      0     139    1482 
   Size:        139    1343
 
SfoI GGC'GCC

Cuts at:      0     495    1482 
   Size:        495     987
 
SmaI CCC'GGG

Cuts at:      0    1225    1482 
   Size:       1225     257
 
SphI G_CATG'C

Cuts at:      0     825    1482 
   Size:        825     657
 
StuI AGG'CCT

Cuts at:      0     709    1328    1482 
   Size:        709     619     154

  Fragments arranged by size: 

                 709     619     154
 
StyI C'CwwG_G

Cuts at:      0     974    1100    1482 
   Size:        974     126     382

  Fragments arranged by size: 

                 974     382     126
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     974    1482 
   Size:        974     508
 
TatI w'GTAC_w

Cuts at:      0     161    1482 
   Size:        161    1321
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     762     794    1482 
   Size:        762      32     688

  Fragments arranged by size: 

                 762     688      32
 
XmaI C'CCGG_G

Cuts at:      0    1223    1482 
   Size:       1223     259
 
XmnI GAAnn'nnTTC

Cuts at:      0     892    1482 
   Size:        892     590
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AleI     AlfI     AloI     ApaI
     AvaI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI Bme1580I
    BmgBI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BseRI
    BseYI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV     FspI    HaeII    Hin4I   HincII  HindIII     KasI     MmeI
     MscI     MslI   MspA1I     NarI     NcoI     NspI     PfoI     PmlI
    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI     SapI    SexAI
     SfcI     SfoI     SmaI     SphI     StuI     StyI    TaqII     TatI
     TstI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI    AlwNI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbsI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmrI     BmtI
    BpuEI    BsaBI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI   BstAPI    BstBI  BstZ17I    CspCI     DraI
   DraIII     DrdI     EagI     EciI    EcoRI     FalI     FseI    FspAI
     HpaI     KpnI     MfeI     MluI     NaeI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI     XhoI
     ZraI
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