Restriction map for pF1KE0215
##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaI        BseAI       BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrI        BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstF5I      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DdeI        DraI        DraIII      DrdI        DsaI        
Eam1105I    EarI        Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FokI        FseI        FspI        HgaI        Hin1I       
HincII      HindII      HindIII     HinfI       HpaI        
KasI        Ksp632I     KspI        MaeI        MaeIII      
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tru9I       
Tsp45I      Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I        230  GGTACC               G^GTACC
AccI          322  GTCTAC               GT^[AC][GT]AC
AlwI          135  GATCC                GGATC(4/5)
Aor51HI       278  AGCGCT               AGC^GCT
Asp718I       230  GGTACC               G^GTACC
BanI          230  GGTACC               G^G[CT][GA]CC
BcnI          119  CCCGG                CC^[GC]GG
BsaBI         198  GATGGTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI         234  CCGAGG               C^C[ACGT][ACGT]GG
BsaWI          27  TCCGGT               [AT]^CCGG[AT]
BseRI         190  GAGGAG               GAGGAG(10/8)
BsgI          253  CTGCAC               GTGCAG(16/14)
BsiEI          50  CGGCCG               CG[GA][CT]^CG
BsiYI         234  CCGAGGTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          234  CCGAGGTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I       80  TGTACA               T^GTACA
BsrGI          80  TGTACA               T^GTACA
BstAPI        246  GCACTGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         134  AGATCC               [GA]^GATC[CT]
DpnII         135  GATC                 ^GATC
DraII         126  GGGACCC              [GA]G^G[ACGT]CC[CT]
EaeI           50  CGGCCG               [CT]^GGCC[GA]
EagI           50  CGGCCG               C^GGCCG
EclXI          50  CGGCCG               C^GGCCG
Eco47III      278  AGCGCT               AGC^GCT
Eco52I         50  CGGCCG               C^GGCCG
EcoO109I      126  GGGACCC              [GA]G^G[ACGT]CC[CT]
FauI          180  CCCGC                CCCGC(4/6)
HaeIII         51  GGCC                 GG^CC
HphI          209  TCACC                GGTGA(8/7)
KpnI          230  GGTACC               GGTAC^C
MaeII         149  ACGT                 A^CGT
MamI          198  GATGGTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          135  GATC                 ^GATC
MflI          134  AGATCC               [GA]^GATC[CT]
NciI          119  CCCGG                CC^[GC]GG
NdeII         135  GATC                 ^GATC
NlaIII         36  CATG                 CATG^
NotI           49  GCGGCCGC             GC^GGCCGC
PpuMI         126  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          265  CTGCAG               CTGCA^G
Sau3AI        135  GATC                 ^GATC
SspBI          80  TGTACA               T^GTACA
XhoII         134  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AluI          158  AGCT                 AG^CT
              263  AGCT                
AvaII         101  GGTCC                G^G[AT]CC
              127  GGACC               
BsmFI         126  GGGAC                GGGAC(10/14)
                3  GTCCC               
Cac8I          72  GCTGGC               GC[ACGT]^[ACGT]GC
              251  GCCTGC              
Cfr13I        101  GGTCC                G^G[ACGT]CC
              127  GGACC               
HaeII          75  GGCGCT               [GA]GCGC^[CT]
              278  AGCGCT              
HapII          28  CCGG                 C^CGG
              120  CCGG                
HpaII          28  CCGG                 C^CGG
              120  CCGG                
MspI           28  CCGG                 C^CGG
              120  CCGG                
Sau96I        101  GGTCC                G^G[ACGT]CC
              127  GGACC               
SfcI          141  CTACAG               C^T[GA][CT]AG
              265  CTGCAG              
TspRI         184  CACTG                CA[GC]TG[ACGT][ACGT]^
              247  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AccII       AciI        AfaI        AspHI       BbvI        
BmyI        BsiHKAI     Bsp1286I    BssKI       BstNI       
BstUI       CfoI        EcoRII      Fnu4HI      HhaI        
HinP1I      ItaI        MboII       MnlI        MvaI        
MvnI        MwoI        NlaIV       RsaI        ScrFI       
TseI        
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