Restriction map for pF1KB6402
 (Linear) (Six-base) MAPSORT of: KIBB6402.seq  Check: 4079  from: 1  to: 999



KIBB6402.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     258     999 
   Size:        258     741
 
AccI GT'mk_AC

Cuts at:      0     500     999 
   Size:        500     499
 
AlwNI CAG_nnn'CTG

Cuts at:      0     495     999 
   Size:        495     504
 
ApaI G_GGCC'C

Cuts at:      0     731     999 
   Size:        731     268
 
ApoI r'AATT_y

Cuts at:      0     259     999 
   Size:        259     740
 
BanII G_rGCy'C

Cuts at:      0     731     999 
   Size:        731     268
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     821     999 
   Size:        821     178
 
BbvCI CC'TCA_GC

Cuts at:      0      45     975     999 
   Size:         45     930      24

  Fragments arranged by size: 

                 930      45      24
 
BclI T'GATC_A

Cuts at:      0      63     999 
   Size:         63     936
 
BglII A'GATC_T

Cuts at:      0     346     999 
   Size:        346     653
 
Bme1580I G_kGCm'C

Cuts at:      0     731     999 
   Size:        731     268
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     473     999 
   Size:        473     526
 
Bpu10I CC'TnA_GC

Cuts at:      0      45     686     975     999 
   Size:         45     641     289      24

  Fragments arranged by size: 

                 641     289      45      24
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     500     999 
   Size:        500     499
 
BsaAI yAC'GTr

Cuts at:      0     375     933     999 
   Size:        375     558      66

  Fragments arranged by size: 

                 558     375      66
 
BsaWI w'CCGG_w

Cuts at:      0     455     950     999 
   Size:        455     495      49

  Fragments arranged by size: 

                 495     455      49
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     156     999 
   Size:        156     843
 
BseYI C'CCAG_C

Cuts at:      0     902     999 
   Size:        902      97
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     999 
   Size:        166     833
 
BsiHKAI G_wGCw'C

Cuts at:      0     438     999 
   Size:        438     561
 
Bsp1286I G_dGCh'C

Cuts at:      0     438     731     999 
   Size:        438     293     268

  Fragments arranged by size: 

                 438     293     268
 
BspEI T'CCGG_A

Cuts at:      0     455     999 
   Size:        455     544
 
BspHI T'CATG_A

Cuts at:      0     756     999 
   Size:        756     243
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     258     742     999 
   Size:        258     484     257

  Fragments arranged by size: 

                 484     258     257
 
BsrDI GCAATG_nn'

Cuts at:      0     528     540     999 
   Size:        528      12     459

  Fragments arranged by size: 

                 528     459      12
 
BsrFI r'CCGG_y

Cuts at:      0     667     999 
   Size:        667     332
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     351     999 
   Size:        351     648
 
BstYI r'GATC_y

Cuts at:      0     346     999 
   Size:        346     653
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     115     999 
   Size:        115     884
 
BtsI GCAGTG_nn'

Cuts at:      0     155     816     999 
   Size:        155     661     183

  Fragments arranged by size: 

                 661     183     155
 
DraIII CAC_nnn'GTG

Cuts at:      0     484     999 
   Size:        484     515
 
EaeI y'GGCC_r

Cuts at:      0     224     941     999 
   Size:        224     717      58

  Fragments arranged by size: 

                 717     224      58
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     706     999 
   Size:        706     293
 
EcoO109I rG'GnC_Cy

Cuts at:      0     339     699     727     999 
   Size:        339     360      28     272

  Fragments arranged by size: 

                 360     339     272      28
 
EcoRI G'AATT_C

Cuts at:      0     259     999 
   Size:        259     740
 
MscI TGG'CCA

Cuts at:      0     226     943     999 
   Size:        226     717      56

  Fragments arranged by size: 

                 717     226      56
 
MslI CAynn'nnrTG

Cuts at:      0     169     999 
   Size:        169     830
 
MspA1I CmG'CkG

Cuts at:      0     902     999 
   Size:        902      97
 
NdeI CA'TA_TG

Cuts at:      0     609     999 
   Size:        609     390
 
PfoI T'CCnGG_A

Cuts at:      0     317     999 
   Size:        317     682
 
PspOMI G'GGCC_C

Cuts at:      0     727     999 
   Size:        727     272
 
PvuII CAG'CTG

Cuts at:      0     902     999 
   Size:        902      97
 
SfcI C'TryA_G

Cuts at:      0     804     999 
   Size:        804     195
 
SmlI C'TyrA_G

Cuts at:      0     515     999 
   Size:        515     484
 
SnaBI TAC'GTA

Cuts at:      0     933     999 
   Size:        933      66
 
SspI AAT'ATT

Cuts at:      0     883     999 
   Size:        883     116
 
StuI AGG'CCT

Cuts at:      0     584     999 
   Size:        584     415
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     913     999 
   Size:        913      86
 
TatI w'GTAC_w

Cuts at:      0     635     999 
   Size:        635     364
 
XmnI GAAnn'nnTTC

Cuts at:      0     313     999 
   Size:        313     686
 

 Enzymes that do cut:

     AarI     AccI    AlwNI     ApaI     ApoI    BanII     BbsI    BbvCI
     BclI    BglII Bme1580I     BmrI   Bpu10I    BpuEI    BsaAI    BsaWI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BstXI    BstYI    BtgZI     BtsI   DraIII     EaeI
    EcoNI EcoO109I    EcoRI     MscI     MslI   MspA1I     NdeI     PfoI
   PspOMI    PvuII     SfcI     SmlI    SnaBI     SspI     StuI    TaqII
     TatI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI
    BfrBI     BglI     BlpI    BmgBI     BmtI     BplI     BpmI     BsaI
    BsaBI    BsaHI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BsrBI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI     ClaI    CspCI     DraI     DrdI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SpeI     SphI
     SrfI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI
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