Restriction map for pF1KE0502
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BbrPI       
BbsI        BbvCI       BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaWI       BseAI       BseRI       
BsgI        BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DdeI        DraI        DraIII      
DrdI        Eam1105I    EarI        Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        Ksp632I     MamI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        TaqI        
TfiI        Tru9I       Tsp509I     Tth111I     TthHB8I     
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccII         223  CGCG                 CG^CG
AflIII        512  ACGTGT               A^C[GA][CT]GT
BanI          503  GGCGCC               G^G[CT][GA]CC
BbeI          503  GGCGCC               GGCGC^C
BcgI           24  CGAACAGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI           91  CTAG                 C^TAG
BsaAI         511  TACGTG               [CT]AC^GT[GA]
BsaI           53  GAGACC               GGTCTC(1/5)
BsiEI         125  CGGCCG               CG[GA][CT]^CG
BsmAI          53  GAGAC                GTCTC(1/5)
BsrBI         520  GAGCGG               CCGCTC(-3/-3)
BstUI         223  CGCG                 CG^CG
BstXI         173  CCATCCAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII         214  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EagI          125  CGGCCG               C^GGCCG
EclXI         125  CGGCCG               C^GGCCG
Eco52I        125  CGGCCG               C^GGCCG
EcoO109I      214  AGGGCCT              [GA]G^G[ACGT]CC[CT]
HaeII         503  GGCGCC               [GA]GCGC^[CT]
HgaI          553  GACGC                GACGC(5/10)
HphI           75  GGTGA                GGTGA(8/7)
KasI          503  GGCGCC               G^GCGCC
KspI          222  CCGCGG               CCGC^GG
MaeI           91  CTAG                 C^TAG
MvnI          223  CGCG                 CG^CG
NarI          503  GGCGCC               GG^CGCC
NcoI           48  CCATGG               C^CATGG
PflMI         410  CCAGCCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI          118  GAGCTC               GAGCT^C
SacII         222  CCGCGG               CCGC^GG
SfoI          503  GGCGCC               GGC^GCC
SspI          322  AATATT               AAT^ATT
Van91I        410  CCAGCCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           48  CCATGGAGACCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI          503  GGCGCC               G[GA]^CG[CT]C
              553  GACGCC              
AspHI          17  GAGCAC               G[AT]GC[AT]^C
              118  GAGCTC              
AvaII         275  GGTCC                G^G[AT]CC
              435  GGACC               
BcnI          188  CCGGG                CC^[GC]GG
              490  CCCGG               
BpmI          157  CTGGAG               CTGGAG(16/14)
              241  CTGGAG              
BsaHI         503  GGCGCC               G[GA]^CG[CT]C
              553  GACGCC              
BsiHKAI        17  GAGCAC               G[AT]GC[AT]^C
              118  GAGCTC              
BsrI          539  ACTGG                ACTGG(1/-1)
              507  CCAGT               
BstAPI        107  GCAAACGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              573  GCAGTCTGTGC         
CfoI          504  GCGC                 GCG^C
              582  GCGC                
DpnII          78  GATC                 ^GATC
              337  GATC                
EaeI          125  CGGCCG               [CT]^GGCC[GA]
              375  TGGCCG              
HhaI          504  GCGC                 GCG^C
              582  GCGC                
Hin1I         503  GGCGCC               G[GA]^CG[CT]C
              553  GACGCC              
HinP1I        504  GCGC                 G^CGC
              582  GCGC                
HinfI         184  GAGTC                G^A[ACGT]TC
              208  GAGTC               
MaeIII        293  GTGAC                ^GT[ACGT]AC
              593  GTGAC               
MboI           78  GATC                 ^GATC
              337  GATC                
MslI          174  CATCCAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              538  CACTGGAATG          
NciI          188  CCGGG                CC^[GC]GG
              490  CCCGG               
NdeII          78  GATC                 ^GATC
              337  GATC                
NlaIII         49  CATG                 CATG^
              424  CATG                
PleI          184  GAGTC                GAGTC(4/5)
              208  GAGTC               
PstI           32  CTGCAG               CTGCA^G
              571  CTGCAG              
PvuII          35  CAGCTG               CAG^CTG
              568  CAGCTG              
Sau3AI         78  GATC                 ^GATC
              337  GATC                
SfcI           32  CTGCAG               C^T[GA][CT]AG
              571  CTGCAG              
Tsp45I        293  GTGAC                ^GT[GC]AC
              593  GTGAC               
TspRI         372  CAGTG                CA[GC]TG[ACGT][ACGT]^
              538  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AfaI        AluI        BanII       BbvI        
BmyI        BsaJI       BsiYI       BslI        BsmFI       
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DsaI        EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HpaII       
ItaI        MaeII       MnlI        MspA1I      MspI        
MvaI        MwoI        NlaIV       RsaI        Sau96I      
ScrFI       SfaNI       StyI        TseI        
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