Restriction map for pF1KE0501
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbeI        BbsI        BcgI        
BclI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstYI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeIII      MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccII         571  CGCG                 CG^CG
AocI          288  CCTGAGG              CC^T[ACGT]AGG
Asp700I        73  GAAAACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI         447  CACGTG               CAC^GTG
BbvCI         279  CCTCAGC              CCTCAGC(-5/-2)
BciVI         566  GTATCC               GTATCC(6/5)
BfaI          157  CTAG                 C^TAG
BpmI          496  CTGGAG               CTGGAG(16/14)
BsaAI         447  CACGTG               [CT]AC^GT[GA]
BsmAI         506  GAGAC                GTCTC(1/5)
BstF5I        327  CATCC                GGATG(2/0)
BstUI         571  CGCG                 CG^CG
Bsu36I        288  CCTGAGG              CC^T[ACGT]AGG
CfoI          561  GCGC                 GCG^C
Eco81I        288  CCTGAGG              CC^T[ACGT]AGG
EcoNI         156  CCTAGTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       402  CCATGG               C^C[AT][AT]GG
FokI          327  CATCC                GGATG(9/13)
HhaI          561  GCGC                 GCG^C
HinP1I        561  GCGC                 G^CGC
MaeI          157  CTAG                 C^TAG
MspA1I        240  CCGCTG               C[AC]G^C[GT]G
MvnI          571  CGCG                 CG^CG
NcoI          402  CCATGG               C^CATGG
NspI          575  ACATGC               [GA]CATG^[CT]
PmaCI         447  CACGTG               CAC^GTG
PmlI          447  CACGTG               CAC^GTG
PstI          484  CTGCAG               CTGCA^G
SacI           97  GAGCTC               GAGCT^C
SfcI          484  CTGCAG               C^T[GA][CT]AG
StyI          402  CCATGG               C^C[AT][AT]GG
TspRI         580  CAGTG                CA[GC]TG[ACGT][ACGT]^
XmnI           73  GAAAACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AlwNI         146  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              487  CAGTACCTG           
ApaI          106  GGGCCC               GGGCC^C
              533  GGGCCC              
AspHI          97  GAGCTC               G[AT]GC[AT]^C
              316  GAGCAC              
AvaII         169  GGACC                G^G[AT]CC
              254  GGACC               
BanI          584  GGCACC               G^G[CT][GA]CC
              595  GGTGCC              
BbvI          260  GCAGC                GCAGC(8/12)
              521  GCAGC               
BcnI          353  CCGGG                CC^[GC]GG
              469  CCCGG               
BsaBI          54  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              363  GATGGAAATC          
BsiHKAI        97  GAGCTC               G[AT]GC[AT]^C
              316  GAGCAC              
BsmFI         245  GGGAC                GGGAC(10/14)
               39  GTCCC               
BspMI         491  ACCTGC               ACCTGC(4/8)
              145  GCAGGT              
BsrI          130  ACTGG                ACTGG(1/-1)
              462  CCAGT               
BstAPI        486  GCAGTACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              552  GCAGTCCGTGC         
BstXI         296  CCATCAAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              396  CCACGCCCATGG        
DsaI          402  CCATGG               C^C[GA][CT]GG
              477  CCGTGG              
Fnu4HI        260  GCAGC                GC^[ACGT]GC
              521  GCAGC               
HapII         353  CCGG                 C^CGG
              470  CCGG                
HinfI         247  GACTC                G^A[ACGT]TC
              274  GACTC               
HpaII         353  CCGG                 C^CGG
              470  CCGG                
ItaI          260  GCAGC                GC^[ACGT]GC
              521  GCAGC               
MaeII         448  ACGT                 A^CGT
              591  ACGT                
MamI           54  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              363  GATGGAAATC          
MboII         357  GAAGA                GAAGA(8/7)
              360  GAAGA               
MspI          353  CCGG                 C^CGG
              470  CCGG                
NciI          353  CCGGG                CC^[GC]GG
              469  CCCGG               
PleI          247  GACTC                GAGTC(4/5)
              274  GACTC               
TseI          260  GCAGC                G^C[AT]GC
              521  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AfaI        AluI        BanII       BmyI        
BsaJI       BsgI        BsiYI       BslI        Bsp1286I    
BssKI       BstNI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EcoO109I    EcoRII      FauI        
HaeIII      HphI        MboI        MnlI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       
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