Restriction map for pF1KE0496
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflII       
AflIII      AgeI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
AspI        AvrII       BamHI       BbeI        BbrPI       
BbvCI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaHI       BsaWI       
BseAI       BseRI       BsgI        BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
Hin1I       HincII      HindII      HindIII     HinfI       
HpaI        KasI        Ksp632I     MaeII       MfeI        
MluI        MroI        MseI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        PacI        
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SalI        
SapI        ScaI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        TfiI        Tru9I       
Tth111I     XbaI        XcmI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I        608  GGTACC               G^GTACC
AccI          526  GTCTAC               GT^[AC][GT]AC
AcsI          223  GAATTC               [GA]^AATT[CT]
AhdI          296  GACCCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI          388  AGCT                 AG^CT
AlwI          495  GATCC                GGATC(4/5)
ApaI           74  GGGCCC               GGGCC^C
ApoI          223  GAATTC               [GA]^AATT[CT]
Asp718I       608  GGTACC               G^GTACC
AspEI         296  GACCCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         272  TGCGCA               TGC^GCA
BalI          737  TGGCCA               TGG^CCA
BbsI          187  GTCTTC               GAAGAC(2/6)
BciVI         591  GTATCC               GTATCC(6/5)
BcnI          103  CCCGG                CC^[GC]GG
BpuAI         187  GTCTTC               GAAGAC(2/6)
BsaBI         336  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          561  GGTCTC               GGTCTC(1/5)
BsiEI         264  CGACCG               CG[GA][CT]^CG
BspMI          81  ACCTGC               ACCTGC(4/8)
BstAPI        678  GCAGTCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         190  TTCGAA               TT^CGAA
BstXI         422  CCATCAAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         494  AGATCC               [GA]^GATC[CT]
Cac8I         692  GCCTGC               GC[ACGT]^[ACGT]GC
DdeI            5  CTCAG                C^T[ACGT]AG
Eam1105I      296  GACCCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         345  CCTGGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         223  GAATTC               G^AATTC
FspI          272  TGCGCA               TGC^GCA
HaeII         142  AGCGCC               [GA]GCGC^[CT]
KpnI          608  GGTACC               GGTAC^C
MamI          336  GATGTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII         188  TCTTC                GAAGA(8/7)
MflI          494  AGATCC               [GA]^GATC[CT]
MluNI         737  TGGCCA               TGG^CCA
MscI          737  TGGCCA               TGG^CCA
NciI          103  CCCGG                CC^[GC]GG
NspV          190  TTCGAA               TT^CGAA
PaeR7I        715  CTCGAG               C^TCGAG
PflMI         515  CCAGTCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI           83  CTGCAG               CTGCA^G
SbfI           82  CCTGCAGG             CCTGCA^GG
SfuI          190  TTCGAA               TT^CGAA
SmlI          715  CTCGAG               C^T[CT][GA]AG
Sse8387I       82  CCTGCAGG             CCTGCA^GG
StuI           17  AGGCCT               AGG^CCT
Tsp509I       224  AATT                 ^AATT
Van91I        515  CCAGTCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          715  CTCGAG               C^TCGAG
XhoII         494  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AspHI         237  GTGCTC               G[AT]GC[AT]^C
              442  GAGCAC              
AvaI          228  CTCGGG               C^[CT]CG[GA]G
              715  CTCGAG              
AvaII         295  GGACC                G^G[AT]CC
              722  GGTCC               
BanI          608  GGTACC               G^G[CT][GA]CC
              710  GGCACC              
BanII          74  GGGCCC               G[GA]GC[CT]^C
              745  GAGCCC              
BcgI           13  GCAAAGGCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              220  GCAGAATTCTCG        
BfaI           98  CTAG                 C^TAG
              622  CTAG                
BglI           31  GCCACAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              145  GCCAAGGCGGC         
BpmI          564  CTCCAG               CTGGAG(16/14)
              346  CTGGAG              
BsiHKAI       237  GTGCTC               G[AT]GC[AT]^C
              442  GAGCAC              
BsoBI         228  CTCGGG               C^[CT]CG[GA]G
              715  CTCGAG              
CfoI          143  GCGC                 GCG^C
              273  GCGC                
DpnII         400  GATC                 ^GATC
              495  GATC                
EaeI           29  CGGCCA               [CT]^GGCC[GA]
              737  TGGCCA              
EcoT14I        62  CCAAGG               C^C[AT][AT]GG
              146  CCAAGG              
HgaI          508  GACGC                GACGC(5/10)
              687  GCGTC               
HhaI          143  GCGC                 GCG^C
              273  GCGC                
HinP1I        143  GCGC                 G^CGC
              273  GCGC                
HphI           42  GGTGA                GGTGA(8/7)
              631  GGTGA               
MaeI           98  CTAG                 C^TAG
              622  CTAG                
MboI          400  GATC                 ^GATC
              495  GATC                
MslI          423  CATCAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              643  CACTGGAATG          
NdeII         400  GATC                 ^GATC
              495  GATC                
PpuMI         294  GGGACCC              [GA]G^G[AT]CC[CT]
              721  GGGTCCT             
Sau3AI        400  GATC                 ^GATC
              495  GATC                
SfcI           83  CTGCAG               C^T[GA][CT]AG
              174  CTACAG              
StyI           62  CCAAGG               C^C[AT][AT]GG
              146  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AccII       AciI        AfaI        AlwNI       BbvI        
BmyI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       Cfr13I      DraII       DsaI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HpaII       ItaI        KspI        
MaeIII      MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NlaIII      NlaIV       RsaI        
SacII       Sau96I      ScrFI       SfaNI       TaqI        
TseI        Tsp45I      TspRI       TthHB8I     
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