Restriction map for pF1KE0496
 (Linear) (Six-base) MAPSORT of: KIEE0496.seq  Check: 9572  from: 1  to: 756



KIEE0496.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      90     756 
   Size:         90     666
 
AccI GT'mk_AC

Cuts at:      0     527     756 
   Size:        527     229
 
Acc65I G'GTAC_C

Cuts at:      0     608     756 
   Size:        608     148
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     519     756 
   Size:        519     237
 
AhdI GACnn_n'nnGTC

Cuts at:      0     301     756 
   Size:        301     455
 
AlwNI CAG_nnn'CTG

Cuts at:      0      55     521     684     756 
   Size:         55     466     163      72

  Fragments arranged by size: 

                 466     163      72      55
 
ApaI G_GGCC'C

Cuts at:      0      78     756 
   Size:         78     678
 
ApoI r'AATT_y

Cuts at:      0     223     756 
   Size:        223     533
 
AvaI C'yCGr_G

Cuts at:      0     228     715     756 
   Size:        228     487      41

  Fragments arranged by size: 

                 487     228      41
 
BanI G'GyrC_C

Cuts at:      0     608     710     756 
   Size:        608     102      46

  Fragments arranged by size: 

                 608     102      46
 
BanII G_rGCy'C

Cuts at:      0      78     749     756 
   Size:         78     671       7

  Fragments arranged by size: 

                 671      78       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     180     756 
   Size:        180     576
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       2      36     209     243     756 
   Size:          2      34     173      34     513

  Fragments arranged by size: 

                 513     173      34      34       2
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     602     756 
   Size:        602     154
 
BglI GCCn_nnn'nGGC

Cuts at:      0      37     151     756 
   Size:         37     114     605

  Fragments arranged by size: 

                 605     114      37
 
Bme1580I G_kGCm'C

Cuts at:      0      78     756 
   Size:         78     678
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     509     756 
   Size:        509     247
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367     549     756 
   Size:        367     182     207

  Fragments arranged by size: 

                 367     207     182
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     567     756 
   Size:        567     189
 
BsaBI GATnn'nnATC

Cuts at:      0     340     756 
   Size:        340     416
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     436     466     756 
   Size:        436      30     290

  Fragments arranged by size: 

                 436     290      30
 
BsiEI CG_ry'CG

Cuts at:      0     267     756 
   Size:        267     489
 
BsiHKAI G_wGCw'C

Cuts at:      0     241     446     756 
   Size:        241     205     310

  Fragments arranged by size: 

                 310     241     205
 
Bsp1286I G_dGCh'C

Cuts at:      0      78     241     446     749     756 
   Size:         78     163     205     303       7

  Fragments arranged by size: 

                 303     205     163      78       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      90     756 
   Size:         90     666
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     684     756 
   Size:        684      72
 
BstBI TT'CG_AA

Cuts at:      0     191     756 
   Size:        191     565
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     429     756 
   Size:        429     327
 
BstYI r'GATC_y

Cuts at:      0     494     756 
   Size:        494     262
 
BtgI C'CryG_G

Cuts at:      0     255     290     327     603     756 
   Size:        255      35      37     276     153

  Fragments arranged by size: 

                 276     255     153      37      35
 
BtsI GCAGTG_nn'

Cuts at:      0     402     712     756 
   Size:        402     310      44

  Fragments arranged by size: 

                 402     310      44
 
EaeI y'GGCC_r

Cuts at:      0      29     737     756 
   Size:         29     708      19

  Fragments arranged by size: 

                 708      29      19
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     169     756 
   Size:        169     587
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367     519     549     756 
   Size:        367     152      30     207

  Fragments arranged by size: 

                 367     207     152      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     349     756 
   Size:        349     407
 
EcoO109I rG'GnC_Cy

Cuts at:      0      52      74      75     295     722     756 
   Size:         52      22       1     220     427      34

  Fragments arranged by size: 

                 427     220      52      34      22       1
 
EcoRI G'AATT_C

Cuts at:      0     223     756 
   Size:        223     533
 
FspI TGC'GCA

Cuts at:      0     274     756 
   Size:        274     482
 
HaeII r_GCGC'y

Cuts at:      0     146     756 
   Size:        146     610
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     287     319     756 
   Size:        287      32     437

  Fragments arranged by size: 

                 437     287      32
 
KpnI G_GTAC'C

Cuts at:      0     612     756 
   Size:        612     144
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     756 
   Size:        344     412
 
MscI TGG'CCA

Cuts at:      0     739     756 
   Size:        739      17
 
MslI CAynn'nnrTG

Cuts at:      0     427     647     756 
   Size:        427     220     109

  Fragments arranged by size: 

                 427     220     109
 
MspA1I CmG'CkG

Cuts at:      0     292     329     605     756 
   Size:        292      37     276     151

  Fragments arranged by size: 

                 292     276     151      37
 
PasI CC'CwG_GG

Cuts at:      0      49     299     756 
   Size:         49     250     457

  Fragments arranged by size: 

                 457     250      49
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     521     756 
   Size:        521     235
 
PfoI T'CCnGG_A

Cuts at:      0     344     756 
   Size:        344     412
 
PpuMI rG'GwC_Cy

Cuts at:      0     295     722     756 
   Size:        295     427      34

  Fragments arranged by size: 

                 427     295      34
 
PspOMI G'GGCC_C

Cuts at:      0      74     756 
   Size:         74     682
 
PspXI vC'TCGA_Gb

Cuts at:      0     715     756 
   Size:        715      41
 
PstI C_TGCA'G

Cuts at:      0      87     756 
   Size:         87     669
 
SacII CC_GC'GG

Cuts at:      0     293     330     606     756 
   Size:        293      37     276     150

  Fragments arranged by size: 

                 293     276     150      37
 
SanDI GG'GwC_CC

Cuts at:      0     295     756 
   Size:        295     461
 
SbfI CC_TGCA'GG

Cuts at:      0      87     756 
   Size:         87     669
 
SfcI C'TryA_G

Cuts at:      0      83     174     756 
   Size:         83      91     582

  Fragments arranged by size: 

                 582      91      83
 
SmlI C'TyrA_G

Cuts at:      0     715     756 
   Size:        715      41
 
StuI AGG'CCT

Cuts at:      0      19     756 
   Size:         19     737
 
StyI C'CwwG_G

Cuts at:      0      62     146     756 
   Size:         62      84     610

  Fragments arranged by size: 

                 610      84      62
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     528     756 
   Size:        528     228
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     436     468     756 
   Size:        436      32     288

  Fragments arranged by size: 

                 436     288      32
 
XhoI C'TCGA_G

Cuts at:      0     715     756 
   Size:        715      41
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AhdI    AlwNI     ApaI     ApoI
     AvaI     BanI    BanII     BbsI     BcgI    BciVI     BglI Bme1580I
     BmrI     BpmI     BsaI    BsaBI    BsaXI    BsiEI  BsiHKAI Bsp1286I
    BspMI   BstAPI    BstBI    BstXI    BstYI     BtgI     BtsI     EaeI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I
     KpnI     MmeI     MscI     MslI   MspA1I     PasI    PflMI     PfoI
    PpuMI   PspOMI    PspXI     PstI    SacII    SanDI     SbfI     SfcI
     SmlI     StuI     StyI    TaqII     TstI     XhoI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AleI     AlfI
     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstEII  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI  EcoICRI
    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI
     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI     PsrI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI
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