Restriction map for
pF1KB7801
(Linear) (Six-base) MAPSORT of: KIBB7801.seq Check: 8678 from: 1 to: 1557
KIBB7801.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 602 1138 1557
Size: 602 536 419
Fragments arranged by size:
602 536 419
AatII G_ACGT'C
Cuts at: 0 214 1557
Size: 214 1343
AccI GT'mk_AC
Cuts at: 0 346 1557
Size: 346 1211
Acc65I G'GTAC_C
Cuts at: 0 338 1557
Size: 338 1219
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 660 901 1033 1051 1557
Size: 660 241 132 18 506
Fragments arranged by size:
660 506 241 132 18
AleI CACnn'nnGTG
Cuts at: 0 629 1557
Size: 629 928
AlwNI CAG_nnn'CTG
Cuts at: 0 153 1482 1557
Size: 153 1329 75
Fragments arranged by size:
1329 153 75
ApaI G_GGCC'C
Cuts at: 0 273 723 1196 1557
Size: 273 450 473 361
Fragments arranged by size:
473 450 361 273
ApoI r'AATT_y
Cuts at: 0 887 1557
Size: 887 670
AvaI C'yCGr_G
Cuts at: 0 70 266 716 1326 1557
Size: 70 196 450 610 231
Fragments arranged by size:
610 450 231 196 70
BanI G'GyrC_C
Cuts at: 0 219 338 1126 1557
Size: 219 119 788 431
Fragments arranged by size:
788 431 219 119
BanII G_rGCy'C
Cuts at: 0 71 273 723 1196 1250 1333 1557
Size: 71 202 450 473 54 83 224
Fragments arranged by size:
473 450 224 202 83 71 54
BbeI G_GCGC'C
Cuts at: 0 223 1557
Size: 223 1334
BbvCI CC'TCA_GC
Cuts at: 0 824 1557
Size: 824 733
BlpI GC'TnA_GC
Cuts at: 0 52 236 1557
Size: 52 184 1321
Fragments arranged by size:
1321 184 52
Bme1580I G_kGCm'C
Cuts at: 0 273 723 1196 1557
Size: 273 450 473 361
Fragments arranged by size:
473 450 361 273
BmrI ACTGGGnnnn_n'
Cuts at: 0 267 497 577 1557
Size: 267 230 80 980
Fragments arranged by size:
980 267 230 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 553 687 720 982 1042 1411 1468
Size: 31 522 134 33 262 60 369 57
Cuts at: 1468 1519 1557
Size: 51 38
Fragments arranged by size:
522 369 262 134 60 57 51 38
33 31
Bpu10I CC'TnA_GC
Cuts at: 0 824 920 1375 1557
Size: 824 96 455 182
Fragments arranged by size:
824 455 182 96
BsaI GGTCTCn'nnnn_
Cuts at: 0 1432 1557
Size: 1432 125
BsaBI GATnn'nnATC
Cuts at: 0 1409 1557
Size: 1409 148
BsaHI Gr'CG_yC
Cuts at: 0 211 220 1557
Size: 211 9 1337
Fragments arranged by size:
1337 211 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 685 715 1243 1273 1557
Size: 232 30 423 30 528 30 284
Fragments arranged by size:
528 423 284 232 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 274 461 739 749 814 817 883 1327
Size: 274 187 278 10 65 3 66 444
Cuts at: 1327 1557
Size: 230
Fragments arranged by size:
444 278 274 230 187 66 65 10
3
BseYI C'CCAG_C
Cuts at: 0 1225 1336 1344 1352 1557
Size: 1225 111 8 8 205
Fragments arranged by size:
1225 205 111 8 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 695 1557
Size: 652 43 862
Fragments arranged by size:
862 652 43
BsiHKAI G_wGCw'C
Cuts at: 0 242 1557
Size: 242 1315
BsmI GAATG_Cn'
Cuts at: 0 1165 1233 1557
Size: 1165 68 324
Fragments arranged by size:
1165 324 68
Bsp1286I G_dGCh'C
Cuts at: 0 71 242 273 723 1196 1250 1333 1557
Size: 71 171 31 450 473 54 83 224
Fragments arranged by size:
473 450 224 171 83 71 54 31
BspHI T'CATG_A
Cuts at: 0 540 1557
Size: 540 1017
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 602 1108 1138 1289 1557
Size: 602 506 30 151 268
Fragments arranged by size:
602 506 268 151 30
BsrBI CCG'CTC
Cuts at: 0 17 1557
Size: 17 1540
BssSI C'ACGA_G
Cuts at: 0 501 1557
Size: 501 1056
BstAPI GCAn_nnn'nTGC
Cuts at: 0 30 1239 1557
Size: 30 1209 318
Fragments arranged by size:
1209 318 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 453 1215 1557
Size: 453 762 342
Fragments arranged by size:
762 453 342
BstYI r'GATC_y
Cuts at: 0 1270 1410 1557
Size: 1270 140 147
Fragments arranged by size:
1270 147 140
BtgI C'CryG_G
Cuts at: 0 1262 1557
Size: 1262 295
BtsI GCAGTG_nn'
Cuts at: 0 43 636 1557
Size: 43 593 921
Fragments arranged by size:
921 593 43
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 176 211 1557
Size: 176 35 1346
Fragments arranged by size:
1346 176 35
EarI CTCTTCn'nnn_
Cuts at: 0 172 802 1533 1557
Size: 172 630 731 24
Fragments arranged by size:
731 630 172 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 717 1557
Size: 717 840
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 553 660 687 720 901 982 1033
Size: 31 522 107 27 33 181 81 51
Cuts at: 1033 1042 1051 1411 1468 1519 1557
Size: 9 9 360 57 51 38
Fragments arranged by size:
522 360 181 107 81 57 51 51
38 33 31 27 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 256 796 1557
Size: 256 540 761
Fragments arranged by size:
761 540 256
EcoO109I rG'GnC_Cy
Cuts at: 0 84 720 781 812 876 1193 1557
Size: 84 636 61 31 64 317 364
Fragments arranged by size:
636 364 317 84 64 61 31
HaeII r_GCGC'y
Cuts at: 0 223 1493 1557
Size: 223 1270 64
Fragments arranged by size:
1270 223 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 903 935 1303 1335 1557
Size: 903 32 368 32 222
Fragments arranged by size:
903 368 222 32 32
HindIII A'AGCT_T
Cuts at: 0 1063 1557
Size: 1063 494
KasI G'GCGC_C
Cuts at: 0 219 1557
Size: 219 1338
KpnI G_GTAC'C
Cuts at: 0 342 1557
Size: 342 1215
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 1557
Size: 434 1123
MslI CAynn'nnrTG
Cuts at: 0 629 1141 1213 1557
Size: 629 512 72 344
Fragments arranged by size:
629 512 344 72
NarI GG'CG_CC
Cuts at: 0 220 1557
Size: 220 1337
PpuMI rG'GwC_Cy
Cuts at: 0 812 1557
Size: 812 745
PspOMI G'GGCC_C
Cuts at: 0 269 719 1192 1557
Size: 269 450 473 365
Fragments arranged by size:
473 450 365 269
PstI C_TGCA'G
Cuts at: 0 38 773 1244 1475 1557
Size: 38 735 471 231 82
Fragments arranged by size:
735 471 231 82 38
SbfI CC_TGCA'GG
Cuts at: 0 1244 1557
Size: 1244 313
SexAI A'CCwGG_T
Cuts at: 0 1481 1557
Size: 1481 76
SfcI C'TryA_G
Cuts at: 0 34 347 769 1240 1471 1557
Size: 34 313 422 471 231 86
Fragments arranged by size:
471 422 313 231 86 34
SfoI GGC'GCC
Cuts at: 0 221 1557
Size: 221 1336
SmaI CCC'GGG
Cuts at: 0 72 718 1328 1557
Size: 72 646 610 229
Fragments arranged by size:
646 610 229 72
SrfI GCCC'GGGC
Cuts at: 0 1328 1557
Size: 1328 229
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 301 1557
Size: 301 1256
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 232 264 685 717 1557
Size: 232 32 421 32 840
Fragments arranged by size:
840 421 232 32 32
XbaI T'CTAG_A
Cuts at: 0 1023 1557
Size: 1023 534
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1404 1557
Size: 1404 153
XmaI C'CCGG_G
Cuts at: 0 70 716 1326 1557
Size: 70 646 610 231
Fragments arranged by size:
646 610 231 70
ZraI GAC'GTC
Cuts at: 0 212 1557
Size: 212 1345
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AleI AlwNI ApaI
ApoI AvaI BanI BanII BbeI BbvCI BlpI Bme1580I
BmrI BpmI Bpu10I BsaI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BssSI BstAPI BstXI BstYI BtgI BtsI CspCI EarI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI
KpnI MmeI MslI NarI PpuMI PspOMI PstI SbfI
SexAI SfcI SfoI SmaI SrfI TaqII TstI XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AclI AfeI AflII AflIII AgeI AhdI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI
BplI BpuEI BsaAI BsaWI BsiEI BsiWI BsmBI BspEI
BsrDI BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI DraI DraIII DrdI EaeI EagI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
MfeI MluI MscI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TatI Tth111I XhoI
XmnI