Restriction map for pF1KB5956
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AviII       AvrII       BamHI       
BbeI        BbsI        BbvCI       BciVI       BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaHI       BsaWI       BseAI       BsiHKAI     BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HaeII       Hin1I       HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MfeI        MflI        
MluI        MroI        MseI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PflMI       
PinAI       PleI        PmeI        PshAI       PshBI       
Psp1406I    PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Van91I      XbaI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AlwNI         445  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       621  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI        1255  CACGTG               CAC^GTG
BcgI          167  GCAGATGGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          593  TGATCA               T^GATCA
BfaI          383  CTAG                 C^TAG
BsaAI        1255  CACGTG               [CT]AC^GT[GA]
BsaBI         429  GATTACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspMI        1616  ACCTGC               ACCTGC(4/8)
BsrDI        1418  GCAATG               GCAATG(2/0)
FbaI          593  TGATCA               T^GATCA
HincII       1249  GTCAAC               GT[CT]^[GA]AC
HindII       1249  GTCAAC               GT[CT]^[GA]AC
MaeI          383  CTAG                 C^TAG
MaeII        1256  ACGT                 A^CGT
MamI          429  GATTACGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I        764  CTCGAG               C^TCGAG
PmaCI        1255  CACGTG               CAC^GTG
PmlI         1255  CACGTG               CAC^GTG
PpuMI         506  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          345  CTGCAG               CTGCA^G
ScaI         1304  AGTACT               AGT^ACT
SfcI          345  CTGCAG               C^T[GA][CT]AG
StuI          385  AGGCCT               AGG^CCT
Tsp509I      1234  AATT                 ^AATT
XhoI          764  CTCGAG               C^TCGAG
XmnI          621  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AlwI          857  GATCC                GGATC(4/5)
              173  GGATC               
ApaI           51  GGGCCC               GGGCC^C
             1091  GGGCCC              
AspI          769  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1330  GACGGGGTC           
AvaI          764  CTCGAG               C^[CT]CG[GA]G
              837  CTCGGG              
BalI           93  TGGCCA               TGG^CCA
             1556  TGGCCA              
BanI           22  GGTGCC               G^G[CT][GA]CC
             1000  GGCACC              
BcnI          111  CCGGG                CC^[GC]GG
             1262  CCCGG               
BglI          479  GCCTGGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1021  GCCATCTGGGC         
BsaI         1549  GAGACC               GGTCTC(1/5)
             1116  GGTCTC              
BsiEI         359  CGATCG               CG[GA][CT]^CG
              434  CGATCG              
BsmBI         363  CGTCTC               CGTCTC(1/5)
              554  GAGACG              
BsoBI         764  CTCGAG               C^[CT]CG[GA]G
              837  CTCGGG              
CfoI          280  GCGC                 GCG^C
             1470  GCGC                
HapII         111  CCGG                 C^CGG
             1263  CCGG                
HgaI          464  GCGTC                GACGC(5/10)
              773  GCGTC               
HhaI          280  GCGC                 GCG^C
             1470  GCGC                
HinP1I        280  GCGC                 G^CGC
             1470  GCGC                
HinfI         957  GAATC                G^A[ACGT]TC
             1085  GATTC               
HpaII         111  CCGG                 C^CGG
             1263  CCGG                
HphI          591  GGTGA                GGTGA(8/7)
              923  TCACC               
MluNI          93  TGGCCA               TGG^CCA
             1556  TGGCCA              
MscI           93  TGGCCA               TGG^CCA
             1556  TGGCCA              
MslI          749  CACAGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1401  CATCATCGTG          
MspI          111  CCGG                 C^CGG
             1263  CCGG                
NciI          111  CCGGG                CC^[GC]GG
             1262  CCCGG               
NcoI           47  CCATGG               C^CATGG
             1436  CCATGG              
NspI         1362  GCATGC               [GA]CATG^[CT]
             1463  GCATGC              
PflFI         769  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1330  GACGGGGTC           
PvuI          359  CGATCG               CGAT^CG
              434  CGATCG              
SmlI          271  CTCAAG               C^T[CT][GA]AG
              764  CTCGAG              
SphI         1362  GCATGC               GCATG^C
             1463  GCATGC              
TfiI          957  GAATC                G^A[AT]TC
             1085  GATTC               
Tsp45I        747  GTCAC                ^GT[GC]AC
             1343  GTGAC               
Tth111I       769  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1330  GACGGGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AvaII       
BanII       BbvI        BmyI        BpmI        BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       DrdI        DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      ItaI        MaeIII      MboI        
MboII       MnlI        MspA1I      MvaI        MvnI        
MwoI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       StyI        
TaqI        TseI        TspRI       TthHB8I     XcmI        
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