Restriction map for pF1KB5956
 (Linear) (Six-base) MAPSORT of: KIBB5956.seq  Check: 7992  from: 1  to: 1644



KIBB5956.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:45  ..

AleI CACnn'nnGTG

Cuts at:      0     753    1644 
   Size:        753     891
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     586    1644 
   Size:        586    1058
 
AlwNI CAG_nnn'CTG

Cuts at:      0     450    1644 
   Size:        450    1194
 
ApaI G_GGCC'C

Cuts at:      0      55    1095    1644 
   Size:         55    1040     549

  Fragments arranged by size: 

                1040     549      55
 
AvaI C'yCGr_G

Cuts at:      0     764     837    1644 
   Size:        764      73     807

  Fragments arranged by size: 

                 807     764      73
 
BanI G'GyrC_C

Cuts at:      0      22    1000    1644 
   Size:         22     978     644

  Fragments arranged by size: 

                 978     644      22
 
BanII G_rGCy'C

Cuts at:      0      55    1095    1640    1644 
   Size:         55    1040     545       4

  Fragments arranged by size: 

                1040     545      55       4
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     156     190    1644 
   Size:        156      34    1454

  Fragments arranged by size: 

                1454     156      34
 
BclI T'GATC_A

Cuts at:      0     593    1644 
   Size:        593    1051
 
BglI GCCn_nnn'nGGC

Cuts at:      0     485    1027    1644 
   Size:        485     542     617

  Fragments arranged by size: 

                 617     542     485
 
Bme1580I G_kGCm'C

Cuts at:      0      27      55     358     638    1003    1095    1644 
   Size:         27      28     303     280     365      92     549

  Fragments arranged by size: 

                 549     365     303     280      92      28      27
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       8      51    1089    1644 
   Size:          8      43    1038     555

  Fragments arranged by size: 

                1038     555      43       8
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     351     556     802    1644 
   Size:        351     205     246     842

  Fragments arranged by size: 

                 842     351     246     205
 
Bpu10I CC'TnA_GC

Cuts at:      0    1164    1644 
   Size:       1164     480
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256    1644 
   Size:        256    1388
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1122    1543    1644 
   Size:       1122     421     101

  Fragments arranged by size: 

                1122     421     101
 
BsaAI yAC'GTr

Cuts at:      0    1257    1644 
   Size:       1257     387
 
BsaBI GATnn'nnATC

Cuts at:      0     433    1644 
   Size:        433    1211
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     775     805    1644 
   Size:        775      30     839

  Fragments arranged by size: 

                 839     775      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     271    1306    1366    1501    1644 
   Size:        271    1035      60     135     143

  Fragments arranged by size: 

                1035     271     143     135      60
 
BseYI C'CCAG_C

Cuts at:      0      27    1644 
   Size:         27    1617
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1393    1528    1615    1644 
   Size:       1393     135      87      29

  Fragments arranged by size: 

                1393     135      87      29
 
BsiEI CG_ry'CG

Cuts at:      0     362     437    1644 
   Size:        362      75    1207

  Fragments arranged by size: 

                1207     362      75
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     369     548    1644 
   Size:        369     179    1096

  Fragments arranged by size: 

                1096     369     179
 
Bsp1286I G_dGCh'C

Cuts at:      0      27      55     358     638    1003    1095    1640    1644 
   Size:         27      28     303     280     365      92     545       4

  Fragments arranged by size: 

                 545     365     303     280      92      28      27       4
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1625    1644 
   Size:       1625      19
 
BsrDI GCAATG_nn'

Cuts at:      0    1425    1644 
   Size:       1425     219
 
BtgI C'CryG_G

Cuts at:      0      47    1367    1436    1644 
   Size:         47    1320      69     208

  Fragments arranged by size: 

                1320     208      69      47
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     463     744    1114    1644 
   Size:        463     281     370     530

  Fragments arranged by size: 

                 530     463     370     281
 
EaeI y'GGCC_r

Cuts at:      0      93    1019    1295    1556    1644 
   Size:         93     926     276     261      88

  Fragments arranged by size: 

                 926     276     261      93      88
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1588    1644 
   Size:       1588      56
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     351     556     802    1644 
   Size:        351     205     246     842

  Fragments arranged by size: 

                 842     351     246     205
 
EcoO109I rG'GnC_Cy

Cuts at:      0      52     507    1091    1644 
   Size:         52     455     584     553

  Fragments arranged by size: 

                 584     553     455      52
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     528    1644 
   Size:        528    1116
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     106     138     758     790     958     990    1644 
   Size:        106      32     620      32     168      32     654

  Fragments arranged by size: 

                 654     620     168     106      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1251    1644 
   Size:       1251     393
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     479     909     974    1644 
   Size:        155     324     430      65     670

  Fragments arranged by size: 

                 670     430     324     155      65
 
MscI TGG'CCA

Cuts at:      0      95    1558    1644 
   Size:         95    1463      86

  Fragments arranged by size: 

                1463      95      86
 
MslI CAynn'nnrTG

Cuts at:      0     753    1405    1644 
   Size:        753     652     239

  Fragments arranged by size: 

                 753     652     239
 
MspA1I CmG'CkG

Cuts at:      0      34     780    1290    1644 
   Size:         34     746     510     354

  Fragments arranged by size: 

                 746     510     354      34
 
NcoI C'CATG_G

Cuts at:      0      47    1436    1644 
   Size:         47    1389     208

  Fragments arranged by size: 

                1389     208      47
 
NspI r_CATG'y

Cuts at:      0    1366    1467    1644 
   Size:       1366     101     177

  Fragments arranged by size: 

                1366     177     101
 
PfoI T'CCnGG_A

Cuts at:      0    1445    1644 
   Size:       1445     199
 
PmlI CAC'GTG

Cuts at:      0    1257    1644 
   Size:       1257     387
 
PpuMI rG'GwC_Cy

Cuts at:      0     507    1644 
   Size:        507    1137
 
PsiI TTA'TAA

Cuts at:      0     545    1644 
   Size:        545    1099
 
PspOMI G'GGCC_C

Cuts at:      0      51    1091    1644 
   Size:         51    1040     553

  Fragments arranged by size: 

                1040     553      51
 
PstI C_TGCA'G

Cuts at:      0     349    1644 
   Size:        349    1295
 
PvuI CG_AT'CG

Cuts at:      0     362     437    1644 
   Size:        362      75    1207

  Fragments arranged by size: 

                1207     362      75
 
ScaI AGT'ACT

Cuts at:      0    1306    1644 
   Size:       1306     338
 
SfcI C'TryA_G

Cuts at:      0     345    1644 
   Size:        345    1299
 
SmlI C'TyrA_G

Cuts at:      0     271     764    1644 
   Size:        271     493     880

  Fragments arranged by size: 

                 880     493     271
 
SphI G_CATG'C

Cuts at:      0    1366    1467    1644 
   Size:       1366     101     177

  Fragments arranged by size: 

                1366     177     101
 
StuI AGG'CCT

Cuts at:      0     387    1644 
   Size:        387    1257
 
StyI C'CwwG_G

Cuts at:      0      47     638    1436    1553    1644 
   Size:         47     591     798     117      91

  Fragments arranged by size: 

                 798     591     117      91      47
 
TatI w'GTAC_w

Cuts at:      0     599    1304    1644 
   Size:        599     705     340

  Fragments arranged by size: 

                 705     599     340
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     601     633     775     807    1644 
   Size:        601      32     142      32     837

  Fragments arranged by size: 

                 837     601     142      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     772    1333    1644 
   Size:        772     561     311

  Fragments arranged by size: 

                 772     561     311
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      13     309    1404    1443    1566    1644 
   Size:         13     296    1095      39     123      78

  Fragments arranged by size: 

                1095     296     123      78      39      13
 
XhoI C'TCGA_G

Cuts at:      0     764    1644 
   Size:        764     880
 
XmnI GAAnn'nnTTC

Cuts at:      0     625    1644 
   Size:        625    1019
 

 Enzymes that do cut:

     AleI     AlfI    AlwNI     ApaI     AvaI     BanI    BanII     BcgI
     BclI     BglI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsmBI
 Bsp1286I    BspMI    BsrDI     BtgI     DrdI     EaeI     EciI  Eco57MI
 EcoO109I     FalI    Hin4I   HincII     MmeI     MscI     MslI   MspA1I
     NcoI     NspI     PfoI     PmlI    PpuMI     PsiI   PspOMI     PstI
     PvuI     ScaI     SfcI     SmlI     SphI     StuI     StyI     TatI
     TstI  Tth111I     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI    BciVI
    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI    BsaHI    BsaWI
  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EagI
     EarI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PpiI    PshAI    PspXI     PsrI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     SwaI    TaqII     XbaI     XmaI     ZraI
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