Restriction map for pF1KB6708
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstXI       
BstYI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
Eco47III    EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FokI        FseI        FspI        
HaeII       HapII       Hin1I       HincII      HindII      
HindIII     HinfI       HpaI        HpaII       HphI        
KasI        KpnI        KspI        MaeI        MaeII       
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MspI        
MunI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfcI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TfiI        Tru9I       
Tsp45I      Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   40 enzymes) #
###################################

AfaI          190  GTAC                 GT^AC
AocI           48  CCTGAGG              CC^T[ACGT]AGG
AvaII         177  GGACC                G^G[AT]CC
BbsI          223  GAAGAC               GAAGAC(2/6)
BpmI           98  CTGGAG               CTGGAG(16/14)
BpuAI         223  GAAGAC               GAAGAC(2/6)
BsgI          313  CTGCAC               GTGCAG(16/14)
BsiEI          74  CGGCCG               CG[GA][CT]^CG
BssKI         218  CCAGG                ^CC[ACGT]GG
BstNI         218  CCAGG                CC^[AT]GG
Bsu36I         48  CCTGAGG              CC^T[ACGT]AGG
DdeI           49  CTGAG                C^T[ACGT]AG
DpnII         150  GATC                 ^GATC
DraII          44  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI           89  CCATGG               C^C[GA][CT]GG
EagI           74  CGGCCG               C^GGCCG
EclXI          74  CGGCCG               C^GGCCG
Eco52I         74  CGGCCG               C^GGCCG
Eco81I         48  CCTGAGG              CC^T[ACGT]AGG
EcoO109I       44  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRII        218  CCAGG                ^CC[AT]GG
EcoT14I        89  CCATGG               C^C[AT][AT]GG
FauI          341  CCCGC                CCCGC(4/6)
HgaI          296  GCGTC                GACGC(5/10)
MaeIII         12  GTTAC                ^GT[ACGT]AC
MboI          150  GATC                 ^GATC
MvaI          218  CCAGG                CC^[AT]GG
NcoI           89  CCATGG               C^CATGG
NdeII         150  GATC                 ^GATC
NotI           73  GCGGCCGC             GC^GGCCGC
PflMI         372  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         376  CAGCTG               CAG^CTG
RsaI          190  GTAC                 GT^AC
SapI          121  GAAGAGC              GCTCTTC(1/4)
Sau3AI        150  GATC                 ^GATC
ScrFI         218  CCAGG                CC^[ACGT]GG
SfiI           36  GGCCGCGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI           89  CCATGG               C^C[AT][AT]GG
Tsp509I       232  AATT                 ^AATT
Van91I        372  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AlwNI          43  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              373  CAGCAGCTG           
BanI          214  GGCACC               G^G[CT][GA]CC
              366  GGTGCC              
BanII          53  GGGCTC               G[GA]GC[CT]^C
              350  GAGCCC              
BsaJI          89  CCATGG               C^C[ACGT][ACGT]GG
              218  CCAGGG              
BsiYI         340  CCCCGCTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              372  CCAGCAGCTGG         
BslI          340  CCCCGCTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              372  CCAGCAGCTGG         
BsmFI         171  GGGAC                GGGAC(10/14)
              238  GTCCC               
EaeI           35  TGGCCG               [CT]^GGCC[GA]
               74  CGGCCG              
EarI          121  GAAGAG               CTCTTC(1/4)
              280  GAAGAG              
Ksp632I       121  GAAGAG               CTCTTC(1/4)
              280  GAAGAG              
MspA1I        342  CCGCTG               C[AC]G^C[GT]G
              376  CAGCTG              
NlaIII         90  CATG                 CATG^
              153  CATG                
TspRI          66  CAGTG                CA[GC]TG[ACGT][ACGT]^
              114  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BglI        
BmyI        Bsp1286I    BsrI        BstUI       Cac8I       
CfoI        Cfr13I      Fnu4HI      HaeIII      HhaI        
HinP1I      ItaI        MboII       MnlI        MvnI        
MwoI        NlaIV       Sau96I      SfaNI       TseI        
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