Restriction map for pF1KB5316
##################################
# NO CUT ENZYMES (  175 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwNI       AocI        Aor51HI     ApaI        
AscI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BciVI       BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BsiEI       BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       EcoT14I     EcoT22I     FauI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HpaI        HpaII       
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    StuI        StyI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AciI          145  GCGG                 CCGC(-3/-1)
Alw44I       1274  GTGCAC               G^TGCAC
ApaLI        1274  GTGCAC               G^TGCAC
AspHI        1274  GTGCAC               G[AT]GC[AT]^C
AvaII        1301  GGACC                G^G[AT]CC
BbvI          279  GCAGC                GCAGC(8/12)
BcgI          838  CGAACCACTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1227  TGATCA               T^GATCA
BfaI         1291  CTAG                 C^TAG
BmyI         1274  GTGCAC               G[AGT]GC[ACT]^C
BseRI          37  GAGGAG               GAGGAG(10/8)
BsiHKAI      1274  GTGCAC               G[AT]GC[AT]^C
Bsp1286I     1274  GTGCAC               G[AGT]GC[ACT]^C
Bsp1407I      632  TGTACA               T^GTACA
BsrDI         949  GCAATG               GCAATG(2/0)
BsrGI         632  TGTACA               T^GTACA
Cac8I        1181  GCTTGC               GC[ACGT]^[ACGT]GC
Cfr13I       1301  GGACC                G^G[ACGT]CC
DraI          205  TTTAAA               TTT^AAA
EaeI         1218  TGGCCG               [CT]^GGCC[GA]
FbaI         1227  TGATCA               T^GATCA
MaeI         1291  CTAG                 C^TAG
PacI         1105  TTAATTAA             TTAAT^TAA
PleI          904  GACTC                GAGTC(4/5)
PstI          403  CTGCAG               CTGCA^G
SapI         1063  GAAGAGC              GCTCTTC(1/4)
Sau96I       1301  GGACC                G^G[ACGT]CC
ScaI          104  AGTACT               AGT^ACT
SfaNI         442  GATGC                GCATC(5/9)
SmlI          756  CTTGAG               C^T[CT][GA]AG
SspBI         632  TGTACA               T^GTACA
SspI          854  AATATT               AAT^ATT
TseI          279  GCAGC                G^C[AT]GC
XcmI          558  CCAGACTGATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AseI          334  ATTAAT               AT^TAAT
             1104  ATTAAT              
AsnI          334  ATTAAT               AT^TAAT
             1104  ATTAAT              
Asp700I       314  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1000  GAAAAGATTC          
BssKI          51  CCTGG                ^CC[ACGT]GG
             1326  CCAGG               
BstNI          51  CCTGG                CC^[AT]GG
             1326  CCAGG               
EarI         1063  GAAGAG               CTCTTC(1/4)
             1263  GAAGAG              
EcoRI         372  GAATTC               G^AATTC
              409  GAATTC              
EcoRII         51  CCTGG                ^CC[AT]GG
             1326  CCAGG               
Fnu4HI        145  GCGGC                GC^[ACGT]GC
              279  GCAGC               
HaeIII        915  GGCC                 GG^CC
             1219  GGCC                
HincII        243  GTTGAC               GT[CT]^[GA]AC
             1234  GTCAAC              
HindII        243  GTTGAC               GT[CT]^[GA]AC
             1234  GTCAAC              
HphI          184  GGTGA                GGTGA(8/7)
              766  GGTGA               
ItaI          145  GCGGC                GC^[ACGT]GC
              279  GCAGC               
Ksp632I      1063  GAAGAG               CTCTTC(1/4)
             1263  GAAGAG              
MvaI           51  CCTGG                CC^[AT]GG
             1326  CCAGG               
PshBI         334  ATTAAT               AT^TAAT
             1104  ATTAAT              
ScrFI          51  CCTGG                CC^[ACGT]GG
             1326  CCAGG               
SfcI          403  CTGCAG               C^T[GA][CT]AG
             1331  CTGTAG              
TfiI          820  GAATC                G^A[AT]TC
             1005  GATTC               
Tsp45I        307  GTCAC                ^GT[GC]AC
              681  GTCAC               
XmnI          314  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1000  GAAAAGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
BsgI        BsiYI       BslI        BsrI        BstF5I      
DdeI        DpnII       FokI        HindIII     HinfI       
MaeIII      MboI        MboII       MnlI        MseI        
NdeII       NlaIII      RsaI        Sau3AI      Tru9I       
Tsp509I     TspRI       
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