Restriction map for pF1KE0566
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        AlwNI       AocI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BalI        BamHI       BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsiEI       
BsiWI       BsmBI       BsmI        BspDI       BspHI       
BsrBI       BsrDI       BssHII      BssSI       BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
Bsu36I      CelII       ClaI        CpoI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MflI        MluI        
MluNI       MscI        MslI        MspA1I      MunI        
NaeI        NcoI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfcI        SfiI        SfuI        SnaBI       
SpeI        Sse8387I    StyI        Tsp45I      Tth111I     
XbaI        XcmI        XhoII       


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccIII        124  TCCGGA               T^CCGGA
AflIII        716  ACATGT               A^C[GA][CT]GT
Alw44I       1096  GTGCAC               G^TGCAC
AlwI          346  GATCC                GGATC(4/5)
Aor51HI       320  AGCGCT               AGC^GCT
ApaI          230  GGGCCC               GGGCC^C
ApaLI        1096  GTGCAC               G^TGCAC
Asp700I       424  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1096  GTGCAC               G[AT]GC[AT]^C
BanII         230  GGGCCC               G[GA]GC[CT]^C
BpmI           32  CTGGAG               CTGGAG(16/14)
BsaWI         124  TCCGGA               [AT]^CCGG[AT]
BseAI         124  TCCGGA               T^CCGGA
BseRI          91  CTCCTC               GAGGAG(10/8)
BsgI          138  GTGCAG               GTGCAG(16/14)
BsiHKAI      1096  GTGCAC               G[AT]GC[AT]^C
Bsp1407I      577  TGTACA               T^GTACA
BspEI         124  TCCGGA               T^CCGGA
BspLU11I      716  ACATGT               A^CATGT
BspMI          11  ACCTGC               ACCTGC(4/8)
BsrGI         577  TGTACA               T^GTACA
Bst1107I      366  GTATAC               GTA^TAC
BstZ17I       366  GTATAC               GTA^TAC
DdeI          362  CTAAG                C^T[ACGT]AG
DpnII         346  GATC                 ^GATC
DraI          457  TTTAAA               TTT^AAA
EaeI          304  TGGCCG               [CT]^GGCC[GA]
Eco47III      320  AGCGCT               AGC^GCT
EcoT22I       493  ATGCAT               ATGCA^T
MboI          346  GATC                 ^GATC
MboII        1177  GAAGA                GAAGA(8/7)
MroI          124  TCCGGA               T^CCGGA
NdeI         1172  CATATG               CA^TATG
NdeII         346  GATC                 ^GATC
NsiI          493  ATGCAT               ATGCA^T
PaeR7I        114  CTCGAG               C^TCGAG
PflMI          25  CCAAGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         856  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         328  GACTTCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI        346  GATC                 ^GATC
ScaI          130  AGTACT               AGT^ACT
SgrAI          98  CACCGGCG             C[GA]^CCGG[CT]G
SmaI          272  CCCGGG               CCC^GGG
SmlI          114  CTCGAG               C^T[CT][GA]AG
SphI           86  GCATGC               GCATG^C
SspBI         577  TGTACA               T^GTACA
StuI          110  AGGCCT               AGG^CCT
SwaI          456  ATTTAAAT             ATTT^AAAT
Van91I         25  CCAAGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          114  CTCGAG               C^TCGAG
XmaCI         272  CCCGGG               C^CCGGG
XmaI          272  CCCGGG               C^CCGGG
XmnI          424  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AccI          160  GTAGAC               GT^[AC][GT]AC
              366  GTATAC              
AvaII         269  GGTCC                G^G[AT]CC
              857  GGACC               
BsaI          467  GAGACC               GGTCTC(1/5)
              276  GGTCTC              
BsrFI          99  ACCGGC               [GA]^CCGG[CT]
              306  GCCGGT              
BstF5I        285  CATCC                GGATG(2/0)
              606  GGATG               
Cfr10I         99  ACCGGC               [GA]^CCGG[CT]
              306  GCCGGT              
DraII         230  GGGCCCT              [GA]G^G[ACGT]CC[CT]
              856  AGGACCT             
EcoO109I      230  GGGCCCT              [GA]G^G[ACGT]CC[CT]
              856  AGGACCT             
FokI          285  CATCC                GGATG(9/13)
              606  GGATG               
NspI           86  GCATGC               [GA]CATG^[CT]
              716  ACATGT              
PleI          993  GACTC                GAGTC(4/5)
             1065  GAGTC               
SspI          921  AATATT               AAT^ATT
             1130  AATATT              
TaqI          115  TCGA                 T^CGA
              432  TCGA                
TfiI          509  GATTC                G^A[AT]TC
              826  GATTC               
TthHB8I       115  TCGA                 T^CGA
              432  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        ApoI        AvaI        BanI        BbeI        
BbvI        BcnI        BmyI        BsaHI       BsaJI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BstNI       BstUI       
Cac8I       CfoI        Cfr13I      EcoRII      FauI        
Fnu4HI      HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        MnlI        MseI        
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NlaIII      NlaIV       RsaI        Sau96I      
ScrFI       SfaNI       SfoI        Tru9I       TseI        
Tsp509I     TspRI       
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