Restriction map for pF1KE0566
 (Linear) (Six-base) MAPSORT of: KIEE0566.seq  Check: 1502  from: 1  to: 1221



KIEE0566.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AccI GT'mk_AC

Cuts at:      0     161     367    1221 
   Size:        161     206     854

  Fragments arranged by size: 

                 854     206     161
 
AfeI AGC'GCT

Cuts at:      0     322    1221 
   Size:        322     899
 
AflIII A'CryG_T

Cuts at:      0     716    1221 
   Size:        716     505
 
ApaI G_GGCC'C

Cuts at:      0     234    1221 
   Size:        234     987
 
ApaLI G'TGCA_C

Cuts at:      0    1096    1221 
   Size:       1096     125
 
ApoI r'AATT_y

Cuts at:      0     454     764     844     877    1054    1076    1150    1221 
   Size:        454     310      80      33     177      22      74      71

  Fragments arranged by size: 

                 454     310     177      80      74      71      33      22
            
 
AvaI C'yCGr_G

Cuts at:      0     114     206     272     279    1221 
   Size:        114      92      66       7     942

  Fragments arranged by size: 

                 942     114      92      66       7
 
BanI G'GyrC_C

Cuts at:      0      55     175     296     309    1221 
   Size:         55     120     121      13     912

  Fragments arranged by size: 

                 912     121     120      55      13
 
BanII G_rGCy'C

Cuts at:      0     234    1221 
   Size:        234     987
 
BbeI G_GCGC'C

Cuts at:      0      59     179     300    1221 
   Size:         59     120     121     921

  Fragments arranged by size: 

                 921     121     120      59
 
BfrBI ATG'CAT

Cuts at:      0     495    1221 
   Size:        495     726
 
Bme1580I G_kGCm'C

Cuts at:      0     234     314    1100    1221 
   Size:        234      80     786     121

  Fragments arranged by size: 

                 786     234     121      80
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     143     391     439    1221 
   Size:        143     248      48     782

  Fragments arranged by size: 

                 782     248     143      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53    1221 
   Size:         53    1168
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     282     461    1221 
   Size:        282     179     760

  Fragments arranged by size: 

                 760     282     179
 
BsaHI Gr'CG_yC

Cuts at:      0      56     103     176     297    1221 
   Size:         56      47      73     121     924

  Fragments arranged by size: 

                 924     121      73      56      47
 
BsaWI w'CCGG_w

Cuts at:      0     124    1221 
   Size:        124    1097
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      82    1221 
   Size:         82    1139
 
BseYI C'CCAG_C

Cuts at:      0     227    1221 
   Size:        227     994
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159    1221 
   Size:        159    1062
 
BsiHKAI G_wGCw'C

Cuts at:      0    1100    1221 
   Size:       1100     121
 
Bsp1286I G_dGCh'C

Cuts at:      0     234     314    1100    1221 
   Size:        234      80     786     121

  Fragments arranged by size: 

                 786     234     121      80
 
BspEI T'CCGG_A

Cuts at:      0     124    1221 
   Size:        124    1097
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      20    1221 
   Size:         20    1201
 
BsrFI r'CCGG_y

Cuts at:      0      99     306    1221 
   Size:         99     207     915

  Fragments arranged by size: 

                 915     207      99
 
BsrGI T'GTAC_A

Cuts at:      0     577    1221 
   Size:        577     644
 
BstZ17I GTA'TAC

Cuts at:      0     368    1221 
   Size:        368     853
 
BtsI GCAGTG_nn'

Cuts at:      0    1094    1221 
   Size:       1094     127
 
DraI TTT'AAA

Cuts at:      0     459    1221 
   Size:        459     762
 
EaeI y'GGCC_r

Cuts at:      0     304    1221 
   Size:        304     917
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53    1221 
   Size:         53    1168
 
EcoO109I rG'GnC_Cy

Cuts at:      0     231     857    1221 
   Size:        231     626     364

  Fragments arranged by size: 

                 626     364     231
 
HaeII r_GCGC'y

Cuts at:      0      59     179     241     300     324     635    1221 
   Size:         59     120      62      59      24     311     586

  Fragments arranged by size: 

                 586     311     120      62      59      59      24
 
KasI G'GCGC_C

Cuts at:      0      55     175     296    1221 
   Size:         55     120     121     925

  Fragments arranged by size: 

                 925     121     120      55
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     588     618    1221 
   Size:        403     185      30     603

  Fragments arranged by size: 

                 603     403     185      30
 
NarI GG'CG_CC

Cuts at:      0      56     176     297    1221 
   Size:         56     120     121     924

  Fragments arranged by size: 

                 924     121     120      56
 
NdeI CA'TA_TG

Cuts at:      0    1173    1221 
   Size:       1173      48
 
NsiI A_TGCA'T

Cuts at:      0     497    1221 
   Size:        497     724
 
NspI r_CATG'y

Cuts at:      0      90     720    1221 
   Size:         90     630     501

  Fragments arranged by size: 

                 630     501      90
 
PciI A'CATG_T

Cuts at:      0     716    1221 
   Size:        716     505
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      31    1221 
   Size:         31    1190
 
PfoI T'CCnGG_A

Cuts at:      0      66    1221 
   Size:         66    1155
 
PpuMI rG'GwC_Cy

Cuts at:      0     857    1221 
   Size:        857     364
 
PshAI GACnn'nnGTC

Cuts at:      0     332    1221 
   Size:        332     889
 
PsiI TTA'TAA

Cuts at:      0    1121    1221 
   Size:       1121     100
 
PspOMI G'GGCC_C

Cuts at:      0     230    1221 
   Size:        230     991
 
ScaI AGT'ACT

Cuts at:      0     132    1221 
   Size:        132    1089
 
SfoI GGC'GCC

Cuts at:      0      57     177     298    1221 
   Size:         57     120     121     923

  Fragments arranged by size: 

                 923     121     120      57
 
SgrAI Cr'CCGG_yG

Cuts at:      0      99    1221 
   Size:         99    1122
 
SmaI CCC'GGG

Cuts at:      0     274    1221 
   Size:        274     947
 
SmlI C'TyrA_G

Cuts at:      0     114    1221 
   Size:        114    1107
 
SphI G_CATG'C

Cuts at:      0      90    1221 
   Size:         90    1131
 
SspI AAT'ATT

Cuts at:      0     923    1132    1221 
   Size:        923     209      89

  Fragments arranged by size: 

                 923     209      89
 
StuI AGG'CCT

Cuts at:      0     112    1221 
   Size:        112    1109
 
SwaI ATTT'AAAT

Cuts at:      0     459    1221 
   Size:        459     762
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     125     729    1221 
   Size:        125     604     492

  Fragments arranged by size: 

                 604     492     125
 
TatI w'GTAC_w

Cuts at:      0     130     517     577    1221 
   Size:        130     387      60     644

  Fragments arranged by size: 

                 644     387     130      60
 
XhoI C'TCGA_G

Cuts at:      0     114    1221 
   Size:        114    1107
 
XmaI C'CCGG_G

Cuts at:      0     272    1221 
   Size:        272     949
 
XmnI GAAnn'nnTTC

Cuts at:      0     428    1221 
   Size:        428     793
 

 Enzymes that do cut:

     AccI     AfeI   AflIII     ApaI    ApaLI     ApoI     AvaI     BanI
    BanII     BbeI    BfrBI Bme1580I     BmrI     BpmI     BsaI    BsaHI
    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI
    BsrFI    BsrGI  BstZ17I     BtsI     DraI     EaeI  Eco57MI EcoO109I
    HaeII     KasI     MmeI     NarI     NdeI     NsiI     NspI     PciI
    PflMI     PfoI    PpuMI    PshAI     PsiI   PspOMI     ScaI     SfoI
    SgrAI     SmaI     SmlI     SphI     SspI     StuI     SwaI    TaqII
     TatI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BsrBI
    BsrDI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PmeI     PmlI     PpiI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SgfI    SnaBI
     SpeI     SrfI     StyI     TstI  Tth111I     XbaI     XcmI     ZraI
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