Restriction map for pF1KB8443
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       AvrII       BbrPI       
BcgI        BlnI        BsaAI       BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Cfr10I      ClaI        CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FseI        FspI        HgaI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MslI        
MunI        MvnI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SspBI       StuI        SwaI        Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AciI         1689  GCGG                 CCGC(-3/-1)
AcsI          953  AAATTT               [GA]^AATT[CT]
AcyI         1302  GGCGCC               G[GA]^CG[CT]C
AfaI         1137  GTAC                 GT^AC
AflII        1569  CTTAAG               C^TTAAG
AflIII        853  ACATGT               A^C[GA][CT]GT
ApaI          290  GGGCCC               GGGCC^C
ApoI          953  AAATTT               [GA]^AATT[CT]
AspHI         180  GAGCTC               G[AT]GC[AT]^C
AvaI            5  CTCGGG               C^[CT]CG[GA]G
BalI          330  TGGCCA               TGG^CCA
BamHI        1536  GGATCC               G^GATCC
BbeI         1302  GGCGCC               GGCGC^C
BbsI         1594  GAAGAC               GAAGAC(2/6)
BbvCI         383  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1398  GTATCC               GTATCC(6/5)
BclI          750  TGATCA               T^GATCA
BcnI          428  CCCGG                CC^[GC]GG
BfrI         1569  CTTAAG               C^TTAAG
BglI          271  GCCCCCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1617  AGATCT               A^GATCT
BlpI           25  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       25  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1594  GAAGAC               GAAGAC(2/6)
BsaHI        1302  GGCGCC               G[GA]^CG[CT]C
BsgI         1132  GTGCAG               GTGCAG(16/14)
BsiHKAI       180  GAGCTC               G[AT]GC[AT]^C
BsoBI           5  CTCGGG               C^[CT]CG[GA]G
BspLU11I      853  ACATGT               A^CATGT
BspMI        1096  ACCTGC               ACCTGC(4/8)
CelII          25  GCTCAGC              GC^T[ACGT]AGC
DrdI         1422  GACTCAAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI          750  TGATCA               T^GATCA
HapII         429  CCGG                 C^CGG
Hin1I        1302  GGCGCC               G[GA]^CG[CT]C
HpaII         429  CCGG                 C^CGG
HphI         1448  TCACC                GGTGA(8/7)
KasI         1302  GGCGCC               G^GCGCC
MaeII        1548  ACGT                 A^CGT
MluNI         330  TGGCCA               TGG^CCA
MscI          330  TGGCCA               TGG^CCA
MspA1I       1736  CAGCTG               C[AC]G^C[GT]G
MspI          429  CCGG                 C^CGG
NarI         1302  GGCGCC               GG^CGCC
NciI          428  CCCGG                CC^[GC]GG
NcoI           63  CCATGG               C^CATGG
NspI          853  ACATGT               [GA]CATG^[CT]
PpuMI         579  GGGTCCT              [GA]G^G[AT]CC[CT]
PvuII        1736  CAGCTG               CAG^CTG
RsaI         1137  GTAC                 GT^AC
SacI          180  GAGCTC               GAGCT^C
SfcI          743  CTGTAG               C^T[GA][CT]AG
SfoI         1302  GGCGCC               GGC^GCC
SpeI         1678  ACTAGT               A^CTAGT
SspI          494  AATATT               AAT^ATT
TfiI         1639  GAATC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwNI          45  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              323  CAGAGTCTG           
BfaI         1663  CTAG                 C^TAG
             1679  CTAG                
BsaI         1258  GAGACC               GGTCTC(1/5)
             1720  GAGACC              
BspHI        1143  TCATGA               T^CATGA
             1268  TCATGA              
BsrDI         597  CATTGC               GCAATG(2/0)
              623  GCAATG              
BstYI        1536  GGATCC               [GA]^GATC[CT]
             1617  AGATCT              
Cac8I         834  GCTGGC               GC[ACGT]^[ACGT]GC
             1100  GCAAGC              
CfoI         1303  GCGC                 GCG^C
             1731  GCGC                
DraII         290  GGGCCCT              [GA]G^G[ACGT]CC[CT]
              579  GGGTCCT             
DsaI           63  CCATGG               C^C[GA][CT]GG
             1579  CCGTGG              
EaeI          330  TGGCCA               [CT]^GGCC[GA]
              430  CGGCCA              
EarI         1204  GAAGAG               CTCTTC(1/4)
             1602  GAAGAG              
EcoO109I      290  GGGCCCT              [GA]G^G[ACGT]CC[CT]
              579  GGGTCCT             
EcoT14I        63  CCATGG               C^C[AT][AT]GG
               84  CCAAGG              
HaeII        1302  GGCGCC               [GA]GCGC^[CT]
             1730  AGCGCC              
HhaI         1303  GCGC                 GCG^C
             1731  GCGC                
HinP1I       1303  GCGC                 G^CGC
             1731  GCGC                
Ksp632I      1204  GAAGAG               CTCTTC(1/4)
             1602  GAAGAG              
MaeI         1663  CTAG                 C^TAG
             1679  CTAG                
MflI         1536  GGATCC               [GA]^GATC[CT]
             1617  AGATCT              
RcaI         1143  TCATGA               T^CATGA
             1268  TCATGA              
StyI           63  CCATGG               C^C[AT][AT]GG
               84  CCAAGG              
XhoII        1536  GGATCC               [GA]^GATC[CT]
             1617  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AluI        AlwI        AocI        AspI        AvaII       
BanI        BanII       BbvI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstXI       Bsu36I      Cfr13I      DdeI        
DpnII       Eco81I      EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       ItaI        MaeIII      MboI        
MboII       MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PflFI       PleI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     
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