Restriction map for pF1KB6380
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BglI        BlnI        BpmI        BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MspA1I      MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp45I      TspRI       Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AcsI            7  GAATTC               [GA]^AATT[CT]
AlwNI         239  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI            7  GAATTC               [GA]^AATT[CT]
Asp700I       822  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         714  GAGCAC               G[AT]GC[AT]^C
AvaII         364  GGACC                G^G[AT]CC
BalI          782  TGGCCA               TGG^CCA
BanI          694  GGTGCC               G^G[CT][GA]CC
BcnI          641  CCGGG                CC^[GC]GG
BfaI          460  CTAG                 C^TAG
BglII         870  AGATCT               A^GATCT
BlpI          879  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      879  GCTGAGC              GC^T[ACGT]AGC
BsaAI          52  TACGTG               [CT]AC^GT[GA]
BsiEI         834  CGACCG               CG[GA][CT]^CG
BsiHKAI       714  GAGCAC               G[AT]GC[AT]^C
BsmFI         363  GGGAC                GGGAC(10/14)
BsmI          170  GCATTC               GAATGC(1/-1)
BstYI         870  AGATCT               [GA]^GATC[CT]
Cac8I         343  GCCAGC               GC[ACGT]^[ACGT]GC
CelII         879  GCTGAGC              GC^T[ACGT]AGC
EaeI          782  TGGCCA               [CT]^GGCC[GA]
EcoRI           7  GAATTC               G^AATTC
HapII         641  CCGG                 C^CGG
HgaI          274  GACGC                GACGC(5/10)
HpaII         641  CCGG                 C^CGG
MaeI          460  CTAG                 C^TAG
MaeIII        532  GTAAC                ^GT[ACGT]AC
MflI          870  AGATCT               [GA]^GATC[CT]
MluNI         782  TGGCCA               TGG^CCA
MscI          782  TGGCCA               TGG^CCA
MslI          495  CACCAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          641  CCGG                 C^CGG
MwoI          668  GCATGAGCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          641  CCGGG                CC^[GC]GG
NlaIII        669  CATG                 CATG^
PleI           13  GACTC                GAGTC(4/5)
PstI          278  CTGCAG               CTGCA^G
SfcI          278  CTGCAG               C^T[GA][CT]AG
SmlI          721  CTTGAG               C^T[CT][GA]AG
TfiI          241  GAATC                G^A[AT]TC
XhoII         870  AGATCT               [GA]^GATC[CT]
XmnI          822  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI          389  GTAC                 GT^AC
              810  GTAC                
AlwI          301  GGATC                GGATC(4/5)
              843  GGATC               
BbsI           19  GAAGAC               GAAGAC(2/6)
              188  GAAGAC              
BbvI          674  GCAGC                GCAGC(8/12)
              277  GCTGC               
BmyI          379  GTGCCC               G[AGT]GC[ACT]^C
              714  GAGCAC              
BpuAI          19  GAAGAC               GAAGAC(2/6)
              188  GAAGAC              
BsaJI         144  CCAAGG               C^C[ACGT][ACGT]GG
              497  CCAAGG              
BsiYI          58  CCGTCGGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              641  CCGGGTCAGGG         
BslI           58  CCGTCGGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              641  CCGGGTCAGGG         
BsmAI         412  GAGAC                GTCTC(1/5)
              421  GAGAC               
Bsp1286I      379  GTGCCC               G[AGT]GC[ACT]^C
              714  GAGCAC              
BstNI         179  CCAGG                CC^[AT]GG
              314  CCAGG               
EcoRII        179  CCAGG                ^CC[AT]GG
              314  CCAGG               
EcoT14I       144  CCAAGG               C^C[AT][AT]GG
              497  CCAAGG              
Fnu4HI        277  GCTGC                GC^[ACGT]GC
              674  GCAGC               
HinfI          13  GACTC                G^A[ACGT]TC
              241  GAATC               
ItaI          277  GCTGC                GC^[ACGT]GC
              674  GCAGC               
MaeII          31  ACGT                 A^CGT
               53  ACGT                
MvaI          179  CCAGG                CC^[AT]GG
              314  CCAGG               
NlaIV         363  GGGACC               GG[ACGT]^[ACGT]CC
              694  GGTGCC              
RsaI          389  GTAC                 GT^AC
              810  GTAC                
SapI          124  GAAGAGC              GCTCTTC(1/4)
              454  GAAGAGC             
StyI          144  CCAAGG               C^C[AT][AT]GG
              497  CCAAGG              
TseI          277  GCTGC                G^C[AT]GC
              674  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AluI        BseRI       BsrI        BssKI       BstF5I      
Cfr13I      DdeI        DpnII       EarI        FokI        
HaeIII      HphI        Ksp632I     MboI        MboII       
MnlI        MseI        NdeII       Sau3AI      Sau96I      
ScrFI       SfaNI       TaqI        Tru9I       Tsp509I     
TthHB8I     
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