Restriction map for pF1KE0032
 (Linear) (Six-base) MAPSORT of: KIEE0032.seq  Check: 2170  from: 1  to: 1821



KIEE0032.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     300    1821 
   Size:        300    1521
 
AccI GT'mk_AC

Cuts at:      0     477    1821 
   Size:        477    1344
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     729    1753    1820    1821 
   Size:        729    1024      67       1

  Fragments arranged by size: 

                1024     729      67       1
 
AhdI GACnn_n'nnGTC

Cuts at:      0     546    1821 
   Size:        546    1275
 
AleI CACnn'nnGTG

Cuts at:      0     493     635     889    1009    1821 
   Size:        493     142     254     120     812

  Fragments arranged by size: 

                 812     493     254     142     120
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     447     479    1821 
   Size:        447      32    1342

  Fragments arranged by size: 

                1342     447      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     138     315    1167    1821 
   Size:        138     177     852     654

  Fragments arranged by size: 

                 852     654     177     138
 
ApaI G_GGCC'C

Cuts at:      0    1485    1821 
   Size:       1485     336
 
ApoI r'AATT_y

Cuts at:      0     371    1225    1567    1821 
   Size:        371     854     342     254

  Fragments arranged by size: 

                 854     371     342     254
 
AvaI C'yCGr_G

Cuts at:      0     126    1478    1789    1821 
   Size:        126    1352     311      32

  Fragments arranged by size: 

                1352     311     126      32
 
BamHI G'GATC_C

Cuts at:      0     626    1386    1821 
   Size:        626     760     435

  Fragments arranged by size: 

                 760     626     435
 
BanI G'GyrC_C

Cuts at:      0     340    1154    1821 
   Size:        340     814     667

  Fragments arranged by size: 

                 814     667     340
 
BanII G_rGCy'C

Cuts at:      0     425    1485    1731    1790    1821 
   Size:        425    1060     246      59      31

  Fragments arranged by size: 

                1060     425     246      59      31
 
BbeI G_GCGC'C

Cuts at:      0     344    1821 
   Size:        344    1477
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     887    1134    1821 
   Size:        887     247     687

  Fragments arranged by size: 

                 887     687     247
 
BbvCI CC'TCA_GC

Cuts at:      0     568    1357    1821 
   Size:        568     789     464

  Fragments arranged by size: 

                 789     568     464
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     190     224    1821 
   Size:        190      34    1597

  Fragments arranged by size: 

                1597     190      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     599    1070    1297    1521    1665    1821 
   Size:        599     471     227     224     144     156

  Fragments arranged by size: 

                 599     471     227     224     156     144
 
BlpI GC'TnA_GC

Cuts at:      0     220     987    1591    1821 
   Size:        220     767     604     230

  Fragments arranged by size: 

                 767     604     230     220
 
Bme1580I G_kGCm'C

Cuts at:      0    1485    1821 
   Size:       1485     336
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      70     786    1623    1821 
   Size:         70     716     837     198

  Fragments arranged by size: 

                 837     716     198      70
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     773    1732    1821 
   Size:        773     959      89

  Fragments arranged by size: 

                 959     773      89
 
Bpu10I CC'TnA_GC

Cuts at:      0     419     568    1357    1821 
   Size:        419     149     789     464

  Fragments arranged by size: 

                 789     464     419     149
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1510    1821 
   Size:       1510     311
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     169    1821 
   Size:        169    1652
 
BsaHI Gr'CG_yC

Cuts at:      0     341    1821 
   Size:        341    1480
 
BsaWI w'CCGG_w

Cuts at:      0     290    1821 
   Size:        290    1531
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     490     520    1821 
   Size:        490      30    1301

  Fragments arranged by size: 

                1301     490      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1099    1378    1597    1699    1807    1821 
   Size:       1099     279     219     102     108      14

  Fragments arranged by size: 

                1099     279     219     108     102      14
 
BseYI C'CCAG_C

Cuts at:      0      93     315     809    1516    1821 
   Size:         93     222     494     707     305

  Fragments arranged by size: 

                 707     494     305     222      93
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     785    1433    1821 
   Size:        184     601     648     388

  Fragments arranged by size: 

                 648     601     388     184
 
BsiHKAI G_wGCw'C

Cuts at:      0    1790    1821 
   Size:       1790      31
 
Bsp1286I G_dGCh'C

Cuts at:      0     425    1485    1731    1790    1821 
   Size:        425    1060     246      59      31

  Fragments arranged by size: 

                1060     425     246      59      31
 
BspEI T'CCGG_A

Cuts at:      0     290    1821 
   Size:        290    1531
 
BspHI T'CATG_A

Cuts at:      0    1250    1821 
   Size:       1250     571
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     300     617    1361    1703    1821 
   Size:        300     317     744     342     118

  Fragments arranged by size: 

                 744     342     317     300     118
 
BsrBI CCG'CTC

Cuts at:      0      17    1821 
   Size:         17    1804
 
BsrDI GCAATG_nn'

Cuts at:      0     977    1821 
   Size:        977     844
 
BsrFI r'CCGG_y

Cuts at:      0     161     582     612     769    1821 
   Size:        161     421      30     157    1052

  Fragments arranged by size: 

                1052     421     161     157      30
 
BssSI C'ACGA_G

Cuts at:      0     885    1821 
   Size:        885     936
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1695    1821 
   Size:       1695     126
 
BstEII G'GTnAC_C

Cuts at:      0    1348    1821 
   Size:       1348     473
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1011    1821 
   Size:       1011     810
 
BstYI r'GATC_y

Cuts at:      0     626    1256    1386    1778    1821 
   Size:        626     630     130     392      43

  Fragments arranged by size: 

                 630     626     392     130      43
 
BtgI C'CryG_G

Cuts at:      0     630     933    1004    1821 
   Size:        630     303      71     817

  Fragments arranged by size: 

                 817     630     303      71
 
BtsI GCAGTG_nn'

Cuts at:      0     111     500    1821 
   Size:        111     389    1321

  Fragments arranged by size: 

                1321     389     111
 
DrdI GACnn_nn'nnGTC

Cuts at:      0       9    1821 
   Size:          9    1812
 
EaeI y'GGCC_r

Cuts at:      0     467    1013    1666    1821 
   Size:        467     546     653     155

  Fragments arranged by size: 

                 653     546     467     155
 
EarI CTCTTCn'nnn_

Cuts at:      0     934    1574    1796    1821 
   Size:        934     640     222      25

  Fragments arranged by size: 

                 934     640     222      25
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     148    1441    1821 
   Size:        148    1293     380

  Fragments arranged by size: 

                1293     380     148
 
EcoICRI GAG'CTC

Cuts at:      0    1788    1821 
   Size:       1788      33
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     729     773    1732    1753    1820    1821 
   Size:        729      44     959      21      67       1

  Fragments arranged by size: 

                 959     729      67      44      21       1
 
EcoO109I rG'GnC_Cy

Cuts at:      0     483    1050    1482    1821 
   Size:        483     567     432     339

  Fragments arranged by size: 

                 567     483     432     339
 
EcoRI G'AATT_C

Cuts at:      0     371    1225    1821 
   Size:        371     854     596

  Fragments arranged by size: 

                 854     596     371
 
HaeII r_GCGC'y

Cuts at:      0     344    1768    1821 
   Size:        344    1424      53

  Fragments arranged by size: 

                1424     344      53
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     532     564     985    1017    1320    1352    1821 
   Size:        532      32     421      32     303      32     469

  Fragments arranged by size: 

                 532     469     421     303      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1041    1215    1319    1821 
   Size:       1041     174     104     502

  Fragments arranged by size: 

                1041     502     174     104
 
HpaI GTT'AAC

Cuts at:      0    1319    1821 
   Size:       1319     502
 
KasI G'GCGC_C

Cuts at:      0     340    1821 
   Size:        340    1481
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     563    1821 
   Size:        563    1258
 
MscI TGG'CCA

Cuts at:      0    1015    1821 
   Size:       1015     806
 
MslI CAynn'nnrTG

Cuts at:      0     493     593     635     889    1009    1438    1821 
   Size:        493     100      42     254     120     429     383

  Fragments arranged by size: 

                 493     429     383     254     120     100      42
 
MspA1I CmG'CkG

Cuts at:      0      63     402     991    1520    1671    1701    1821 
   Size:         63     339     589     529     151      30     120

  Fragments arranged by size: 

                 589     529     339     151     120      63      30
 
NaeI GCC'GGC

Cuts at:      0     163     584    1821 
   Size:        163     421    1237

  Fragments arranged by size: 

                1237     421     163
 
NarI GG'CG_CC

Cuts at:      0     341    1821 
   Size:        341    1480
 
NgoMIV G'CCGG_C

Cuts at:      0     161     582    1821 
   Size:        161     421    1239

  Fragments arranged by size: 

                1239     421     161
 
PfoI T'CCnGG_A

Cuts at:      0    1412    1781    1821 
   Size:       1412     369      40

  Fragments arranged by size: 

                1412     369      40
 
PpuMI rG'GwC_Cy

Cuts at:      0     483    1821 
   Size:        483    1338
 
PspOMI G'GGCC_C

Cuts at:      0    1481    1821 
   Size:       1481     340
 
PspXI vC'TCGA_Gb

Cuts at:      0    1789    1821 
   Size:       1789      32
 
PstI C_TGCA'G

Cuts at:      0    1310    1700    1821 
   Size:       1310     390     121

  Fragments arranged by size: 

                1310     390     121
 
PvuII CAG'CTG

Cuts at:      0     991    1520    1701    1821 
   Size:        991     529     181     120

  Fragments arranged by size: 

                 991     529     181     120
 
SacI G_AGCT'C

Cuts at:      0    1790    1821 
   Size:       1790      31
 
ScaI AGT'ACT

Cuts at:      0    1612    1821 
   Size:       1612     209
 
SfcI C'TryA_G

Cuts at:      0     478    1306    1696    1708    1821 
   Size:        478     828     390      12     113

  Fragments arranged by size: 

                 828     478     390     113      12
 
SfoI GGC'GCC

Cuts at:      0     342    1821 
   Size:        342    1479
 
SmaI CCC'GGG

Cuts at:      0     128    1480    1821 
   Size:        128    1352     341

  Fragments arranged by size: 

                1352     341     128
 
SmlI C'TyrA_G

Cuts at:      0    1525    1789    1821 
   Size:       1525     264      32

  Fragments arranged by size: 

                1525     264      32
 
StuI AGG'CCT

Cuts at:      0    1291    1821 
   Size:       1291     530
 
StyI C'CwwG_G

Cuts at:      0      88     689     921    1244    1821 
   Size:         88     601     232     323     577

  Fragments arranged by size: 

                 601     577     323     232      88
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      45     130     475     718    1821 
   Size:         45      85     345     243    1103

  Fragments arranged by size: 

                1103     345     243      85      45
 
TatI w'GTAC_w

Cuts at:      0    1367    1490    1610    1821 
   Size:       1367     123     120     211

  Fragments arranged by size: 

                1367     211     123     120
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     618     650    1821 
   Size:        618      32    1171

  Fragments arranged by size: 

                1171     618      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     853    1821 
   Size:        853     968
 
XhoI C'TCGA_G

Cuts at:      0    1789    1821 
   Size:       1789      32
 
XmaI C'CCGG_G

Cuts at:      0     126    1478    1821 
   Size:        126    1352     343

  Fragments arranged by size: 

                1352     343     126
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AhdI     AleI     AloI    AlwNI     ApaI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI     BglI     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI   BstEII    BstXI    BstYI     BtgI     BtsI     DrdI     EaeI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    HaeII    Hin4I
   HincII     HpaI     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     PfoI    PpuMI   PspOMI    PspXI     PstI    PvuII
     SacI     ScaI     SfcI     SfoI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XhoI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AlfI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BciVI     BclI
    BfrBI    BglII    BmgBI     BmtI     BplI    BsaAI    BsaBI    BsiEI
    BsiWI     BsmI    BsmBI    BsrGI   BssHII    BstBI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     EagI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     KpnI     MfeI     MluI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     XbaI     XcmI     XmnI     ZraI
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