Restriction map for pF1KE0486
 (Linear) (Six-base) MAPSORT of: KIEE0486.seq  Check: 553  from: 1  to: 540



KIEE0486.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     540 
   Size:        151     389
 
AleI CACnn'nnGTG

Cuts at:      0     349     540 
   Size:        349     191
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     473     540 
   Size:        473      67
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     481     513     540 
   Size:        481      32      27

  Fragments arranged by size: 

                 481      32      27
 
AlwNI CAG_nnn'CTG

Cuts at:      0      90     310     435     540 
   Size:         90     220     125     105

  Fragments arranged by size: 

                 220     125     105      90
 
ApaI G_GGCC'C

Cuts at:      0     523     540 
   Size:        523      17
 
BanII G_rGCy'C

Cuts at:      0     523     540 
   Size:        523      17
 
BglI GCCn_nnn'nGGC

Cuts at:      0     424     540 
   Size:        424     116
 
BglII A'GATC_T

Cuts at:      0     452     540 
   Size:        452      88
 
Bme1580I G_kGCm'C

Cuts at:      0      67     473     523     540 
   Size:         67     406      50      17

  Fragments arranged by size: 

                 406      67      50      17
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     540 
   Size:        540
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     217     418     540 
   Size:        150      67     201     122

  Fragments arranged by size: 

                 201     150     122      67
 
BsaAI yAC'GTr

Cuts at:      0     468     540 
   Size:        468      72
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     106     136     540 
   Size:        106      30     404

  Fragments arranged by size: 

                 404     106      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     259     540 
   Size:        259     281
 
BseYI C'CCAG_C

Cuts at:      0     310     540 
   Size:        310     230
 
BsmI GAATG_Cn'

Cuts at:      0     332     540 
   Size:        332     208
 
Bsp1286I G_dGCh'C

Cuts at:      0      67     473     523     540 
   Size:         67     406      50      17

  Fragments arranged by size: 

                 406      67      50      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     500     540 
   Size:        500      40
 
BsrFI r'CCGG_y

Cuts at:      0     338     540 
   Size:        338     202
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      57     540 
   Size:         57     483
 
BstYI r'GATC_y

Cuts at:      0     452     540 
   Size:        452      88
 
BtgI C'CryG_G

Cuts at:      0     347     422     540 
   Size:        347      75     118

  Fragments arranged by size: 

                 347     118      75
 
BtsI GCAGTG_nn'

Cuts at:      0     324     540 
   Size:        324     216
 
DraIII CAC_nnn'GTG

Cuts at:      0     348     540 
   Size:        348     192
 
EaeI y'GGCC_r

Cuts at:      0      44     462     540 
   Size:         44     418      78

  Fragments arranged by size: 

                 418      78      44
 
EarI CTCTTCn'nnn_

Cuts at:      0      83     540 
   Size:         83     457
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     109     540 
   Size:        109     431
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     151     217     418     540 
   Size:        150       1      66     201     122

  Fragments arranged by size: 

                 201     150     122      66       1
 
EcoO109I rG'GnC_Cy

Cuts at:      0      14     519     520     534     540 
   Size:         14     505       1      14       6

  Fragments arranged by size: 

                 505      14      14       6       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     156     540 
   Size:        156     384
 
HaeII r_GCGC'y

Cuts at:      0     435     540 
   Size:        435     105
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     106     138     540 
   Size:        106      32     402

  Fragments arranged by size: 

                 402     106      32
 
MscI TGG'CCA

Cuts at:      0     464     540 
   Size:        464      76
 
MslI CAynn'nnrTG

Cuts at:      0     263     349     540 
   Size:        263      86     191

  Fragments arranged by size: 

                 263     191      86
 
MspA1I CmG'CkG

Cuts at:      0      90     540 
   Size:         90     450
 
NcoI C'CATG_G

Cuts at:      0     422     540 
   Size:        422     118
 
PmlI CAC'GTG

Cuts at:      0     468     540 
   Size:        468      72
 
PpuMI rG'GwC_Cy

Cuts at:      0     534     540 
   Size:        534       6
 
PspOMI G'GGCC_C

Cuts at:      0     519     540 
   Size:        519      21
 
PstI C_TGCA'G

Cuts at:      0      86     331     452     540 
   Size:         86     245     121      88

  Fragments arranged by size: 

                 245     121      88      86
 
PvuII CAG'CTG

Cuts at:      0      90     540 
   Size:         90     450
 
SapI GCTCTTCn'nnn_

Cuts at:      0      83     540 
   Size:         83     457
 
SfcI C'TryA_G

Cuts at:      0      82     327     448     540 
   Size:         82     245     121      92

  Fragments arranged by size: 

                 245     121      92      82
 
StyI C'CwwG_G

Cuts at:      0     422     540 
   Size:        422     118
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      54      57     276     540 
   Size:         54       3     219     264

  Fragments arranged by size: 

                 264     219      54       3
 

 Enzymes that do cut:

     AcuI     AleI     AlfI     AloI    AlwNI     ApaI    BanII     BglI
    BglII Bme1580I     BplI     BpmI    BsaAI    BsaXI    BseRI    BseYI
     BsmI Bsp1286I    BspMI    BsrFI    BstXI    BstYI     BtgI     BtsI
   DraIII     EaeI     EarI     EciI  Eco57MI EcoO109I     FalI    HaeII
    Hin4I     MscI     MslI   MspA1I     NcoI     PmlI    PpuMI   PspOMI
     PstI    PvuII     SapI     SfcI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BlpI    BmgBI     BmrI     BmtI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI     DrdI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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