Restriction map for pF1KB9881
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AvrII       BbrPI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BsaAI       BsaBI       BsiWI       BsmBI       BsmFI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     ClaI        CpoI        DraI        Eam1105I    
Eco47III    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        HincII      HindII      HindIII     HpaI        
MamI        MfeI        MluI        MseI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SapI        
SbfI        SexAI       SfuI        SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tth111I     XbaI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I       1667  GGTACC               G^GTACC
AccIII         69  TCCGGA               T^CCGGA
AflIII        569  ACGTGT               A^C[GA][CT]GT
AocI         1061  CCTCAGG              CC^T[ACGT]AGG
Asp718I      1667  GGTACC               G^GTACC
BalI          821  TGGCCA               TGG^CCA
BamHI        1056  GGATCC               G^GATCC
BbsI         1579  GAAGAC               GAAGAC(2/6)
BbvCI        1417  GCTGAGG              CCTCAGC(-5/-2)
BfaI         1045  CTAG                 C^TAG
BlpI          123  GCTGAGC              GC^T[ACGT]AGC
BpmI          973  CTGGAG               CTGGAG(16/14)
Bpu1102I      123  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1579  GAAGAC               GAAGAC(2/6)
BsaWI          69  TCCGGA               [AT]^CCGG[AT]
BseAI          69  TCCGGA               T^CCGGA
BspEI          69  TCCGGA               T^CCGGA
Bsu36I       1061  CCTCAGG              CC^T[ACGT]AGG
CelII         123  GCTGAGC              GC^T[ACGT]AGC
DrdI          647  GACGGCCGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1061  CCTCAGG              CC^T[ACGT]AGG
EcoNI          75  CCTGCGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI          885  GGCCGGCC             GGCCGG^CC
KpnI         1667  GGTACC               GGTAC^C
MaeI         1045  CTAG                 C^TAG
MluNI         821  TGGCCA               TGG^CCA
MroI           69  TCCGGA               T^CCGGA
MscI          821  TGGCCA               TGG^CCA
MslI          846  CACCCGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI          930  GCATGT               [GA]CATG^[CT]
PaeR7I        229  CTCGAG               C^TCGAG
PpuMI         872  GGGTCCT              [GA]G^G[AT]CC[CT]
SacI         1395  GAGCTC               GAGCT^C
ScaI          827  AGTACT               AGT^ACT
SfiI          757  GGCCAAGCCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI         379  CGCCGGCG             C[GA]^CCGG[CT]G
Tsp509I      1322  AATT                 ^AATT
XhoI          229  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwNI         667  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              814  CAGGCCCTG           
ApaI            3  GGGCCC               GGGCC^C
             1427  GGGCCC              
AviII          77  TGCGCA               TGC^GCA
              314  TGCGCA              
BbeI          383  GGCGCC               GGCGC^C
              456  GGCGCC              
BsaI          586  GAGACC               GGTCTC(1/5)
             1078  GGTCTC              
BstYI         260  AGATCC               [GA]^GATC[CT]
             1056  GGATCC              
DraIII        388  CACGGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1096  CACTTTGTG           
FspI           77  TGCGCA               TGC^GCA
              314  TGCGCA              
KasI          383  GGCGCC               G^GCGCC
              456  GGCGCC              
KspI          473  CCGCGG               CCGC^GG
              623  CCGCGG              
MaeII          25  ACGT                 A^CGT
              569  ACGT                
MflI          260  AGATCC               [GA]^GATC[CT]
             1056  GGATCC              
NarI          383  GGCGCC               GG^CGCC
              456  GGCGCC              
PleI         1525  GACTC                GAGTC(4/5)
              862  GAGTC               
SacII         473  CCGCGG               CCGC^GG
              623  CCGCGG              
SfaNI         290  GCATC                GCATC(5/9)
              966  GCATC               
SfoI          383  GGCGCC               GGC^GCC
              456  GGCGCC              
StuI          836  AGGCCT               AGG^CCT
             1065  AGGCCT              
TaqI          230  TCGA                 T^CGA
             1776  TCGA                
TfiI           66  GAATC                G^A[AT]TC
             1531  GATTC               
TthHB8I       230  TCGA                 T^CGA
             1776  TCGA                
XcmI          356  CCAACGGCTGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              809  CCACCCAGGCCCTGG     
XhoII         260  AGATCC               [GA]^GATC[CT]
             1056  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BglI        BmyI        
BsaHI       BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssHII      
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EagI        EarI        
EclXI       Eco52I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeIII      MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NcoI        NdeII       NgoMIV      NlaIII      
NlaIV       PflMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        SmlI        
StyI        TseI        Tsp45I      TspRI       Van91I      
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