Restriction map for pF1KB9881
 (Linear) (Six-base) MAPSORT of: KIBB9881.seq  Check: 1292  from: 1  to: 1797



KIBB9881.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     199     984    1797 
   Size:        199     785     813

  Fragments arranged by size: 

                 813     785     199
 
Acc65I G'GTAC_C

Cuts at:      0    1667    1797 
   Size:       1667     130
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157    1797 
   Size:        157    1640
 
AflIII A'CryG_T

Cuts at:      0     569    1797 
   Size:        569    1228
 
AlwNI CAG_nnn'CTG

Cuts at:      0     672     819    1797 
   Size:        672     147     978

  Fragments arranged by size: 

                 978     672     147
 
ApaI G_GGCC'C

Cuts at:      0       7    1431    1797 
   Size:          7    1424     366

  Fragments arranged by size: 

                1424     366       7
 
AvaI C'yCGr_G

Cuts at:      0     229     521     918    1797 
   Size:        229     292     397     879

  Fragments arranged by size: 

                 879     397     292     229
 
BamHI G'GATC_C

Cuts at:      0    1056    1797 
   Size:       1056     741
 
BanI G'GyrC_C

Cuts at:      0     383     456     477     958    1471    1614    1667    1797 
   Size:        383      73      21     481     513     143      53     130

  Fragments arranged by size: 

                 513     481     383     143     130      73      53      21
            
 
BanII G_rGCy'C

Cuts at:      0       7    1399    1431    1797 
   Size:          7    1392      32     366

  Fragments arranged by size: 

                1392     366      32       7
 
BbeI G_GCGC'C

Cuts at:      0     387     460    1797 
   Size:        387      73    1337

  Fragments arranged by size: 

                1337     387      73
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1586    1797 
   Size:       1586     211
 
BbvCI CC'TCA_GC

Cuts at:      0    1418    1797 
   Size:       1418     379
 
BglI GCCn_nnn'nGGC

Cuts at:      0     157     469     764    1117    1765    1797 
   Size:        157     312     295     353     648      32

  Fragments arranged by size: 

                 648     353     312     295     157      32
 
BlpI GC'TnA_GC

Cuts at:      0     124    1797 
   Size:        124    1673
 
Bme1580I G_kGCm'C

Cuts at:      0       7     482    1431    1476    1495    1797 
   Size:          7     475     949      45      19     302

  Fragments arranged by size: 

                 949     475     302      45      19       7
 
BmgBI CAC'GTC

Cuts at:      0     570    1797 
   Size:        570    1227
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     897    1434    1482    1797 
   Size:        897     537      48     315

  Fragments arranged by size: 

                 897     537     315      48
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1413    1416    1797 
   Size:       1413       3     381

  Fragments arranged by size: 

                1413     381       3
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     994    1797 
   Size:        994     803
 
Bpu10I CC'TnA_GC

Cuts at:      0     673    1418    1797 
   Size:        673     745     379

  Fragments arranged by size: 

                 745     673     379
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283    1012    1186    1797 
   Size:        283     729     174     611

  Fragments arranged by size: 

                 729     611     283     174
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     580    1084    1797 
   Size:        580     504     713

  Fragments arranged by size: 

                 713     580     504
 
BsaHI Gr'CG_yC

Cuts at:      0     160     384     457    1797 
   Size:        160     224      73    1340

  Fragments arranged by size: 

                1340     224     160      73
 
BsaWI w'CCGG_w

Cuts at:      0      69    1797 
   Size:         69    1728
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     157     187    1660    1690    1797 
   Size:        157      30    1473      30     107

  Fragments arranged by size: 

                1473     157     107      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      76     340     541    1237    1240    1243    1246    1258 
   Size:         76     264     201     696       3       3       3      12

Cuts at:   1258    1261    1288    1291    1303    1306    1309    1312    1351 
   Size:          3      27       3      12       3       3       3      39

Cuts at:   1351    1360    1363    1389    1393    1405    1612    1797 
   Size:          9       3      26       4      12     207     185

  Fragments arranged by size: 

                 696     264     207     201     185      76      39      27
                  26      12      12      12       9       4       3       3
                   3       3       3       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     330     665    1074    1624    1797 
   Size:        330     335     409     550     173

  Fragments arranged by size: 

                 550     409     335     330     173
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     955    1078    1720    1797 
   Size:        955     123     642      77

  Fragments arranged by size: 

                 955     642     123      77
 
BsiEI CG_ry'CG

Cuts at:      0     473     652     891    1797 
   Size:        473     179     239     906

  Fragments arranged by size: 

                 906     473     239     179
 
BsiHKAI G_wGCw'C

Cuts at:      0     130     505    1399    1797 
   Size:        130     375     894     398

  Fragments arranged by size: 

                 894     398     375     130
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     130     482     505    1399    1431    1476    1495 
   Size:          7     123     352      23     894      32      45      19

Cuts at:   1495    1797 
   Size:        302

  Fragments arranged by size: 

                 894     352     302     123      45      32      23      19
                   7
 
BspEI T'CCGG_A

Cuts at:      0      69    1797 
   Size:         69    1728
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      83     199     984    1797 
   Size:         83     116     785     813

  Fragments arranged by size: 

                 813     785     116      83
 
BsrFI r'CCGG_y

Cuts at:      0     271     279     380     730     763     886    1447    1797 
   Size:        271       8     101     350      33     123     561     350

  Fragments arranged by size: 

                 561     350     350     271     123     101      33       8
            
 
BssHII G'CGCG_C

Cuts at:      0     147     425     516    1797 
   Size:        147     278      91    1281

  Fragments arranged by size: 

                1281     278     147      91
 
BstYI r'GATC_y

Cuts at:      0     260    1056    1797 
   Size:        260     796     741

  Fragments arranged by size: 

                 796     741     260
 
Bsu36I CC'TnA_GG

Cuts at:      0    1062    1797 
   Size:       1062     735
 
BtgI C'CryG_G

Cuts at:      0     387     473     623    1034    1640    1655    1797 
   Size:        387      86     150     411     606      15     142

  Fragments arranged by size: 

                 606     411     387     150     142      86      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     228    1797 
   Size:        228    1569
 
BtsI GCAGTG_nn'

Cuts at:      0     498    1127    1797 
   Size:        498     629     670

  Fragments arranged by size: 

                 670     629     498
 
DraIII CAC_nnn'GTG

Cuts at:      0     393    1101    1797 
   Size:        393     708     696

  Fragments arranged by size: 

                 708     696     393
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     653    1797 
   Size:        653    1144
 
EaeI y'GGCC_r

Cuts at:      0     470     545     649     728     740     821     884     888 
   Size:        470      75     104      79      12      81      63       4

Cuts at:    888    1037    1797 
   Size:        149     760

  Fragments arranged by size: 

                 760     470     149     104      81      79      75      63
                  12       4
 
EagI C'GGCC_G

Cuts at:      0     470     649     888    1797 
   Size:        470     179     239     909

  Fragments arranged by size: 

                 909     470     239     179
 
EarI CTCTTCn'nnn_

Cuts at:      0    1214    1235    1247    1265    1328    1370    1541    1556 
   Size:       1214      21      12      18      63      42     171      15

Cuts at:   1556    1797 
   Size:        241

  Fragments arranged by size: 

                1214     241     171      63      42      21      18      15
                  12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     367    1797 
   Size:        367    1430
 
EcoICRI GAG'CTC

Cuts at:      0    1397    1797 
   Size:       1397     400
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     994    1797 
   Size:        157     837     803

  Fragments arranged by size: 

                 837     803     157
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      79    1797 
   Size:         79    1718
 
EcoO109I rG'GnC_Cy

Cuts at:      0       4     816     873    1089    1797 
   Size:          4     812      57     216     708

  Fragments arranged by size: 

                 812     708     216      57       4
 
FseI GG_CCGG'CC

Cuts at:      0     890    1797 
   Size:        890     907
 
FspI TGC'GCA

Cuts at:      0      79     316    1797 
   Size:         79     237    1481

  Fragments arranged by size: 

                1481     237      79
 
HaeII r_GCGC'y

Cuts at:      0     102     313     387     460     581     672    1797 
   Size:        102     211      74      73     121      91    1125

  Fragments arranged by size: 

                1125     211     121     102      91      74      73
 
KasI G'GCGC_C

Cuts at:      0     383     456    1797 
   Size:        383      73    1341

  Fragments arranged by size: 

                1341     383      73
 
KpnI G_GTAC'C

Cuts at:      0    1671    1797 
   Size:       1671     126
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     380    1797 
   Size:        143     237    1417

  Fragments arranged by size: 

                1417     237     143
 
MscI TGG'CCA

Cuts at:      0     823    1797 
   Size:        823     974
 
MslI CAynn'nnrTG

Cuts at:      0     850    1797 
   Size:        850     947
 
MspA1I CmG'CkG

Cuts at:      0      21     407     475     625     723     939     972    1074 
   Size:         21     386      68     150      98     216      33     102

Cuts at:   1074    1797 
   Size:        723

  Fragments arranged by size: 

                 723     386     216     150     102      98      68      33
                  21
 
NaeI GCC'GGC

Cuts at:      0     273     382     732     765     888    1797 
   Size:        273     109     350      33     123     909

  Fragments arranged by size: 

                 909     350     273     123     109      33
 
NarI GG'CG_CC

Cuts at:      0     384     457    1797 
   Size:        384      73    1340

  Fragments arranged by size: 

                1340     384      73
 
NcoI C'CATG_G

Cuts at:      0    1034    1640    1655    1797 
   Size:       1034     606      15     142

  Fragments arranged by size: 

                1034     606     142      15
 
NgoMIV G'CCGG_C

Cuts at:      0     271     380     730     763     886    1797 
   Size:        271     109     350      33     123     911

  Fragments arranged by size: 

                 911     350     271     123     109      33
 
NspI r_CATG'y

Cuts at:      0     934    1797 
   Size:        934     863
 
PasI CC'CwG_GG

Cuts at:      0    1431    1797 
   Size:       1431     366
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      57     362     393     596     819     909    1797 
   Size:         57     305      31     203     223      90     888

  Fragments arranged by size: 

                 888     305     223     203      90      57      31
 
PfoI T'CCnGG_A

Cuts at:      0      29      50    1508    1797 
   Size:         29      21    1458     289

  Fragments arranged by size: 

                1458     289      29      21
 
PpuMI rG'GwC_Cy

Cuts at:      0     873    1797 
   Size:        873     924
 
PspOMI G'GGCC_C

Cuts at:      0       3    1427    1797 
   Size:          3    1424     370

  Fragments arranged by size: 

                1424     370       3
 
PspXI vC'TCGA_Gb

Cuts at:      0     229    1797 
   Size:        229    1568
 
PstI C_TGCA'G

Cuts at:      0     406     861    1073    1797 
   Size:        406     455     212     724

  Fragments arranged by size: 

                 724     455     406     212
 
PvuII CAG'CTG

Cuts at:      0      21     939    1074    1797 
   Size:         21     918     135     723

  Fragments arranged by size: 

                 918     723     135      21
 
SacI G_AGCT'C

Cuts at:      0    1399    1797 
   Size:       1399     398
 
SacII CC_GC'GG

Cuts at:      0     476     626    1797 
   Size:        476     150    1171

  Fragments arranged by size: 

                1171     476     150
 
ScaI AGT'ACT

Cuts at:      0     829    1797 
   Size:        829     968
 
SfcI C'TryA_G

Cuts at:      0     402     857    1069    1797 
   Size:        402     455     212     728

  Fragments arranged by size: 

                 728     455     402     212
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     764    1797 
   Size:        764    1033
 
SfoI GGC'GCC

Cuts at:      0     385     458    1797 
   Size:        385      73    1339

  Fragments arranged by size: 

                1339     385      73
 
SgrAI Cr'CCGG_yG

Cuts at:      0     380    1797 
   Size:        380    1417
 
SmlI C'TyrA_G

Cuts at:      0     229     298    1027    1201    1797 
   Size:        229      69     729     174     596

  Fragments arranged by size: 

                 729     596     229     174      69
 
StuI AGG'CCT

Cuts at:      0     838    1067    1797 
   Size:        838     229     730

  Fragments arranged by size: 

                 838     730     229
 
StyI C'CwwG_G

Cuts at:      0     794     839     908    1034    1640    1655    1797 
   Size:        794      45      69     126     606      15     142

  Fragments arranged by size: 

                 794     606     142     126      69      45      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     679     826    1797 
   Size:        679     147     971

  Fragments arranged by size: 

                 971     679     147
 
TatI w'GTAC_w

Cuts at:      0     827    1797 
   Size:        827     970
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      32      64     951     983    1474    1506    1797 
   Size:         32      32     887      32     491      32     291

  Fragments arranged by size: 

                 887     491     291      32      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     363     816    1797 
   Size:        363     453     981

  Fragments arranged by size: 

                 981     453     363
 
XhoI C'TCGA_G

Cuts at:      0     229    1797 
   Size:        229    1568
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII    AlwNI     ApaI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspMI    BsrFI   BssHII    BstYI   Bsu36I     BtgI    BtgZI
     BtsI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I     FseI     FspI    HaeII     KasI     KpnI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI    PflMI     PfoI    PpuMI   PspOMI    PspXI     PstI
    PvuII     SacI    SacII     ScaI     SfcI     SfiI     SfoI    SgrAI
     SmlI     StuI     StyI    TaqII     TatI     TstI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BcgI    BciVI     BclI    BfrBI    BglII     BmtI    BsaAI
    BsaBI    BsiWI     BsmI    BsmBI    BspHI    BsrBI    BsrDI    BsrGI
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I     ClaI    CspCI
     DraI    EcoRI    EcoRV     FalI    FspAI    Hin4I   HincII  HindIII
     HpaI     MfeI     MluI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI     PsrI
     PvuI    RsrII     SalI    SanDI     SapI     SbfI    SexAI     SgfI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I
     XbaI     XmaI     XmnI     ZraI
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