Restriction map for pF1KE0514
 (Linear) (Six-base) MAPSORT of: KIEE0514.seq  Check: 2062  from: 1  to: 687



KIEE0514.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     322     687 
   Size:        169     153     365

  Fragments arranged by size: 

                 365     169     153
 
AlwNI CAG_nnn'CTG

Cuts at:      0     437     504     687 
   Size:        437      67     183

  Fragments arranged by size: 

                 437     183      67
 
ApaI G_GGCC'C

Cuts at:      0     281     470     687 
   Size:        281     189     217

  Fragments arranged by size: 

                 281     217     189
 
ApaLI G'TGCA_C

Cuts at:      0     376     687 
   Size:        376     311
 
AvaI C'yCGr_G

Cuts at:      0     366     687 
   Size:        366     321
 
BanI G'GyrC_C

Cuts at:      0      64     687 
   Size:         64     623
 
BanII G_rGCy'C

Cuts at:      0     218     281     470     687 
   Size:        218      63     189     217

  Fragments arranged by size: 

                 218     217     189      63
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     447     687 
   Size:        447     240
 
BbvCI CC'TCA_GC

Cuts at:      0     484     687 
   Size:        484     203
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     357     391     687 
   Size:        357      34     296

  Fragments arranged by size: 

                 357     296      34
 
BglII A'GATC_T

Cuts at:      0      98     687 
   Size:         98     589
 
Bme1580I G_kGCm'C

Cuts at:      0     281     380     470     687 
   Size:        281      99      90     217

  Fragments arranged by size: 

                 281     217      99      90
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     669     687 
   Size:        669      18
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     540     687 
   Size:        540     147
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     250     651     687 
   Size:        115     135     401      36

  Fragments arranged by size: 

                 401     135     115      36
 
Bpu10I CC'TnA_GC

Cuts at:      0     484     687 
   Size:        484     203
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      90     193     687 
   Size:         90     103     494

  Fragments arranged by size: 

                 494     103      90
 
BsaAI yAC'GTr

Cuts at:      0     321     687 
   Size:        321     366
 
BsaHI Gr'CG_yC

Cuts at:      0     452     687 
   Size:        452     235
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     124     154     382     412     687 
   Size:        124      30     228      30     275

  Fragments arranged by size: 

                 275     228     124      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     403     643     687 
   Size:        403     240      44

  Fragments arranged by size: 

                 403     240      44
 
BseYI C'CCAG_C

Cuts at:      0     422     504     571     687 
   Size:        422      82      67     116

  Fragments arranged by size: 

                 422     116      82      67
 
BsiEI CG_ry'CG

Cuts at:      0     555     687 
   Size:        555     132
 
BsiHKAI G_wGCw'C

Cuts at:      0     251     269     380     687 
   Size:        251      18     111     307

  Fragments arranged by size: 

                 307     251     111      18
 
Bsp1286I G_dGCh'C

Cuts at:      0     218     251     269     281     380     470     687 
   Size:        218      33      18      12      99      90     217

  Fragments arranged by size: 

                 218     217      99      90      33      18      12
 
BstYI r'GATC_y

Cuts at:      0      98     191     644     662     670     687 
   Size:         98      93     453      18       8      17

  Fragments arranged by size: 

                 453      98      93      18      17       8
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     362     687 
   Size:        362     325
 
DraI TTT'AAA

Cuts at:      0     546     687 
   Size:        546     141
 
DraIII CAC_nnn'GTG

Cuts at:      0     324     687 
   Size:        324     363
 
EarI CTCTTCn'nnn_

Cuts at:      0     602     687 
   Size:        602      85
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     169     250     322     651     687 
   Size:        115      54      81      72     329      36

  Fragments arranged by size: 

                 329     115      81      72      54      36
 
EcoO109I rG'GnC_Cy

Cuts at:      0      74     278     466     467     594     687 
   Size:         74     204     188       1     127      93

  Fragments arranged by size: 

                 204     188     127      93      74       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     234     687 
   Size:        234     453
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     298     330     382     414     687 
   Size:        298      32      52      32     273

  Fragments arranged by size: 

                 298     273      52      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     425     551     687 
   Size:        425     126     136

  Fragments arranged by size: 

                 425     136     126
 
MspA1I CmG'CkG

Cuts at:      0     138     165     256     422     687 
   Size:        138      27      91     166     265

  Fragments arranged by size: 

                 265     166     138      91      27
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     324     504     687 
   Size:        324     180     183

  Fragments arranged by size: 

                 324     183     180
 
PfoI T'CCnGG_A

Cuts at:      0     238     687 
   Size:        238     449
 
PmlI CAC'GTG

Cuts at:      0     321     687 
   Size:        321     366
 
PpuMI rG'GwC_Cy

Cuts at:      0      74     687 
   Size:         74     613
 
PshAI GACnn'nnGTC

Cuts at:      0     451     687 
   Size:        451     236
 
PspOMI G'GGCC_C

Cuts at:      0     277     466     687 
   Size:        277     189     221

  Fragments arranged by size: 

                 277     221     189
 
PvuII CAG'CTG

Cuts at:      0     138     165     687 
   Size:        138      27     522

  Fragments arranged by size: 

                 522     138      27
 
SmlI C'TyrA_G

Cuts at:      0      69     208     687 
   Size:         69     139     479

  Fragments arranged by size: 

                 479     139      69
 
StyI C'CwwG_G

Cuts at:      0     658     687 
   Size:        658      29
 
TatI w'GTAC_w

Cuts at:      0     185     687 
   Size:        185     502
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI    ApaLI     AvaI     BanI    BanII     BbsI
    BbvCI     BcgI    BglII Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaHI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BstYI    BtgZI     DraI   DraIII     EarI  Eco57MI EcoO109I
     FalI    Hin4I     MmeI   MspA1I    PflMI     PfoI     PmlI    PpuMI
    PshAI   PspOMI    PvuII     SmlI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BciVI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmtI     BsaI    BsaBI    BsaWI     BsgI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PpiI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com