Restriction map for pF1KE0509
##################################
# NO CUT ENZYMES (  184 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BmyI        BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiHKAI     BsiWI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraII       DrdI        
Eam1105I    Eco47III    Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FspI        HaeII       HgaI        Hin1I       
HphI        KasI        KpnI        KspI        MaeII       
MaeIII      MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MunI        MvnI        NarI        NciI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AcsI          235  GAATTT               [GA]^AATT[CT]
AlwI           72  GGATC                GGATC(4/5)
ApoI          235  GAATTT               [GA]^AATT[CT]
BfaI          485  CTAG                 C^TAG
BlpI          382  GCTAAGC              GC^T[ACGT]AGC
BpmI           83  CTGGAG               CTGGAG(16/14)
Bpu1102I      382  GCTAAGC              GC^T[ACGT]AGC
BsiEI          12  CGGCCG               CG[GA][CT]^CG
BsrFI          14  GCCGGC               [GA]^CCGG[CT]
BssKI          40  CCTGG                ^CC[ACGT]GG
BstNI          40  CCTGG                CC^[AT]GG
CelII         382  GCTAAGC              GC^T[ACGT]AGC
CfoI            7  GCGC                 GCG^C
Cfr10I         14  GCCGGC               [GA]^CCGG[CT]
Cfr13I         17  GGCCC                G^G[ACGT]CC
DpnII          73  GATC                 ^GATC
DraIII         92  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          287  CCATGG               C^C[GA][CT]GG
EagI           12  CGGCCG               C^GGCCG
EclXI          12  CGGCCG               C^GGCCG
Eco52I         12  CGGCCG               C^GGCCG
EcoRII         40  CCTGG                ^CC[AT]GG
EcoT14I       287  CCATGG               C^C[AT][AT]GG
FseI           13  GGCCGGCC             GGCCGG^CC
HapII          15  CCGG                 C^CGG
HhaI            7  GCGC                 GCG^C
HinP1I          7  GCGC                 G^CGC
HincII        178  GTTAAC               GT[CT]^[GA]AC
HindII        178  GTTAAC               GT[CT]^[GA]AC
HindIII       115  AAGCTT               A^AGCTT
HinfI         268  GAGTC                G^A[ACGT]TC
HpaI          178  GTTAAC               GTT^AAC
HpaII          15  CCGG                 C^CGG
MaeI          485  CTAG                 C^TAG
MboI           73  GATC                 ^GATC
MseI          179  TTAA                 T^TAA
MspI           15  CCGG                 C^CGG
MvaI           40  CCTGG                CC^[AT]GG
NaeI           14  GCCGGC               GCC^GGC
NcoI          287  CCATGG               C^CATGG
NdeII          73  GATC                 ^GATC
NgoMIV         14  GCCGGC               G^CCGGC
NlaIV         435  GGAACC               GG[ACGT]^[ACGT]CC
NspI          387  GCATGT               [GA]CATG^[CT]
PleI          268  GAGTC                GAGTC(4/5)
Sau3AI         73  GATC                 ^GATC
Sau96I         17  GGCCC                G^G[ACGT]CC
ScrFI          40  CCTGG                CC^[ACGT]GG
StyI          287  CCATGG               C^C[AT][AT]GG
Tru9I         179  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BbvI            4  GCTGC                GCAGC(8/12)
              394  GCTGC               
BsiYI          19  CCCTTCAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               35  CCATACCTGGG         
BslI           19  CCCTTCAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               35  CCATACCTGGG         
BsmAI         475  GAGAC                GTCTC(1/5)
              456  GTCTC               
BsrI           82  ACTGG                ACTGG(1/-1)
              191  CCAGT               
BstF5I        344  CATCC                GGATG(2/0)
              452  GGATG               
Cac8I          14  GCCGGC               GC[ACGT]^[ACGT]GC
              397  GCTGGC              
EaeI           12  CGGCCG               [CT]^GGCC[GA]
              442  CGGCCA              
EarI          228  GAAGAG               CTCTTC(1/4)
              496  GAAGAG              
Fnu4HI          4  GCTGC                GC^[ACGT]GC
              394  GCTGC               
FokI          344  CATCC                GGATG(9/13)
              452  GGATG               
ItaI            4  GCTGC                GC^[ACGT]GC
              394  GCTGC               
Ksp632I       228  GAAGAG               CTCTTC(1/4)
              496  GAAGAG              
MwoI            9  GCTCGGCCGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              397  GCTGGCACTGC         
TseI            4  GCTGC                G^C[AT]GC
              394  GCTGC               
Tsp509I       141  AATT                 ^AATT
              236  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AluI        BsaJI       DdeI        HaeIII      MboII       
MnlI        NlaIII      TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com