Restriction map for pF1KB5996
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        AlwI        
AocI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       BamHI       BcgI        BciVI       BclI        
BfrI        BsaBI       BseAI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    Eco81I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tsp509I     
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII         988  CGCG                 CG^CG
AcyI          976  GGCGCC               G[GA]^CG[CT]C
Alw44I         65  GTGCAC               G^TGCAC
AlwNI         241  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1520  GGGCCC               GGGCC^C
ApaLI          65  GTGCAC               G^TGCAC
AvaI           21  CTCGGG               C^[CT]CG[GA]G
AvrII         531  CCTAGG               C^CTAGG
BbeI          976  GGCGCC               GGCGC^C
BbrPI        1408  CACGTG               CAC^GTG
BglII        1032  AGATCT               A^GATCT
BlnI          531  CCTAGG               C^CTAGG
BsaAI        1408  CACGTG               [CT]AC^GT[GA]
BsaHI         976  GGCGCC               G[GA]^CG[CT]C
BsaI         1474  GAGACC               GGTCTC(1/5)
BsaWI        1481  ACCGGA               [AT]^CCGG[AT]
BsoBI          21  CTCGGG               C^[CT]CG[GA]G
BsrDI         548  CATTGC               GCAATG(2/0)
BstBI        1357  TTCGAA               TT^CGAA
BstEII         25  GGTTACC              G^GT[ACGT]ACC
BstPI          25  GGTTACC              G^GT[ACGT]ACC
BstUI         988  CGCG                 CG^CG
BstYI        1032  AGATCT               [GA]^GATC[CT]
DsaI          972  CCGTGG               C^C[GA][CT]GG
EarI          925  CTCTTC               CTCTTC(1/4)
EcoNI        1290  CCTTTATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I        25  GGTTACC              G^GT[ACGT]ACC
HaeII         976  GGCGCC               [GA]GCGC^[CT]
Hin1I         976  GGCGCC               G[GA]^CG[CT]C
HphI         1403  TCACC                GGTGA(8/7)
KasI          976  GGCGCC               G^GCGCC
Ksp632I       925  CTCTTC               CTCTTC(1/4)
MaeII        1409  ACGT                 A^CGT
MflI         1032  AGATCT               [GA]^GATC[CT]
MseI         1435  TTAA                 T^TAA
MvnI          988  CGCG                 CG^CG
NarI          976  GGCGCC               GG^CGCC
NspV         1357  TTCGAA               TT^CGAA
PmaCI        1408  CACGTG               CAC^GTG
PmlI         1408  CACGTG               CAC^GTG
PshAI         991  GACGGCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         1300  GAGCTC               GAGCT^C
SfiI         1521  GGCCCGGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          976  GGCGCC               GGC^GCC
SfuI         1357  TTCGAA               TT^CGAA
Tru9I        1435  TTAA                 T^TAA
Tsp45I        527  GTGAC                ^GT[GC]AC
XcmI           83  CCAAGAAGCCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1032  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AflIII        151  ACATGT               A^C[GA][CT]GT
             1691  ACATGT              
BbsI          300  GAAGAC               GAAGAC(2/6)
             1804  GAAGAC              
BbvCI         199  CCTCAGC              CCTCAGC(-5/-2)
              387  CCTCAGC             
BfaI          532  CTAG                 C^TAG
             1169  CTAG                
BpuAI         300  GAAGAC               GAAGAC(2/6)
             1804  GAAGAC              
BsiEI         415  CGGCCG               CG[GA][CT]^CG
              993  CGGCCG              
BspLU11I      151  ACATGT               A^CATGT
             1691  ACATGT              
BspMI         860  ACCTGC               ACCTGC(4/8)
             1220  GCAGGT              
BsrBI         877  CCGCTC               CCGCTC(-3/-3)
             1629  CCGCTC              
BstXI        1010  CCACCAGCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1733  CCAAGGACATGG        
CfoI          285  GCGC                 GCG^C
              977  GCGC                
DpnII         825  GATC                 ^GATC
             1033  GATC                
EagI          415  CGGCCG               C^GGCCG
              993  CGGCCG              
EclXI         415  CGGCCG               C^GGCCG
              993  CGGCCG              
Eco52I        415  CGGCCG               C^GGCCG
              993  CGGCCG              
HhaI          285  GCGC                 GCG^C
              977  GCGC                
HinP1I        285  GCGC                 G^CGC
              977  GCGC                
MaeI          532  CTAG                 C^TAG
             1169  CTAG                
MaeIII         26  GTTAC                ^GT[ACGT]AC
              527  GTGAC               
MboI          825  GATC                 ^GATC
             1033  GATC                
NaeI          496  GCCGGC               GCC^GGC
             1658  GCCGGC              
NdeII         825  GATC                 ^GATC
             1033  GATC                
NgoMIV        496  GCCGGC               G^CCGGC
             1658  GCCGGC              
PstI         1209  CTGCAG               CTGCA^G
             1554  CTGCAG              
Sau3AI        825  GATC                 ^GATC
             1033  GATC                
TfiI          667  GATTC                G^A[AT]TC
             1190  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AciI        AfaI        AluI        AspHI       AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrFI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CelII       Cfr10I      Cfr13I      DdeI        DraII       
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       ItaI        MboII       MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PvuII       RsaI        
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TaqI        TseI        TspRI       
TthHB8I     Van91I      
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