Restriction map for pF1KB5996
 (Linear) (Six-base) MAPSORT of: KIBB5996.seq  Check: 4058  from: 1  to: 1839



KIBB5996.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:45  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     779    1111    1839 
   Size:        779     332     728

  Fragments arranged by size: 

                 779     728     332
 
AflIII A'CryG_T

Cuts at:      0     151    1691    1839 
   Size:        151    1540     148

  Fragments arranged by size: 

                1540     151     148
 
AleI CACnn'nnGTG

Cuts at:      0    1408    1839 
   Size:       1408     431
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1103    1839 
   Size:       1103     736
 
AlwNI CAG_nnn'CTG

Cuts at:      0     246    1839 
   Size:        246    1593
 
ApaI G_GGCC'C

Cuts at:      0    1524    1839 
   Size:       1524     315
 
ApaLI G'TGCA_C

Cuts at:      0      65    1839 
   Size:         65    1774
 
AvaI C'yCGr_G

Cuts at:      0      21    1839 
   Size:         21    1818
 
AvrII C'CTAG_G

Cuts at:      0     531    1839 
   Size:        531    1308
 
BanI G'GyrC_C

Cuts at:      0       8     194     315     348     404     535     976    1839 
   Size:          8     186     121      33      56     131     441     863

  Fragments arranged by size: 

                 863     441     186     131     121      56      33       8
            
 
BanII G_rGCy'C

Cuts at:      0     511     742     890    1025    1051    1207    1304    1524 
   Size:        511     231     148     135      26     156      97     220

Cuts at:   1524    1625    1682    1839 
   Size:        101      57     157

  Fragments arranged by size: 

                 511     231     220     157     156     148     135     101
                  97      57      26
 
BbeI G_GCGC'C

Cuts at:      0     980    1839 
   Size:        980     859
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     307    1811    1839 
   Size:        307    1504      28

  Fragments arranged by size: 

                1504     307      28
 
BbvCI CC'TCA_GC

Cuts at:      0     200     388    1839 
   Size:        200     188    1451

  Fragments arranged by size: 

                1451     200     188
 
BglI GCCn_nnn'nGGC

Cuts at:      0     992    1180    1528    1839 
   Size:        992     188     348     311

  Fragments arranged by size: 

                 992     348     311     188
 
BglII A'GATC_T

Cuts at:      0    1032    1839 
   Size:       1032     807
 
BlpI GC'TnA_GC

Cuts at:      0     162    1540    1666    1839 
   Size:        162    1378     126     173

  Fragments arranged by size: 

                1378     173     162     126
 
Bme1580I G_kGCm'C

Cuts at:      0      69     372     540    1176    1524    1839 
   Size:         69     303     168     636     348     315

  Fragments arranged by size: 

                 636     348     315     303     168      69
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     334    1839 
   Size:        334    1505
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     215    1044    1587    1839 
   Size:        215     829     543     252

  Fragments arranged by size: 

                 829     543     252     215
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     277     444     598     628     800     868    1095 
   Size:        154     123     167     154      30     172      68     227

Cuts at:   1095    1525    1839 
   Size:        430     314

  Fragments arranged by size: 

                 430     314     227     172     167     154     154     123
                  68      30
 
Bpu10I CC'TnA_GC

Cuts at:      0     200     388    1414    1839 
   Size:        200     188    1026     425

  Fragments arranged by size: 

                1026     425     200     188
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     574     726    1183    1839 
   Size:        574     152     457     656

  Fragments arranged by size: 

                 656     574     457     152
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1468    1839 
   Size:       1468     371
 
BsaAI yAC'GTr

Cuts at:      0    1410    1839 
   Size:       1410     429
 
BsaHI Gr'CG_yC

Cuts at:      0     977    1839 
   Size:        977     862
 
BsaWI w'CCGG_w

Cuts at:      0    1481    1839 
   Size:       1481     358
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     814     844    1839 
   Size:        814      30     995

  Fragments arranged by size: 

                 995     814      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1163    1312    1522    1839 
   Size:       1163     149     210     317

  Fragments arranged by size: 

                1163     317     210     149
 
BseYI C'CCAG_C

Cuts at:      0     310     382     672     735    1319    1380    1839 
   Size:        310      72     290      63     584      61     459

  Fragments arranged by size: 

                 584     459     310     290      72      63      61
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      81      94     352     411    1597    1686    1839 
   Size:         81      13     258      59    1186      89     153

  Fragments arranged by size: 

                1186     258     153      89      81      59      13
 
BsiEI CG_ry'CG

Cuts at:      0     418     996    1839 
   Size:        418     578     843

  Fragments arranged by size: 

                 843     578     418
 
BsiHKAI G_wGCw'C

Cuts at:      0      69    1304    1415    1839 
   Size:         69    1235     111     424

  Fragments arranged by size: 

                1235     424     111      69
 
Bsp1286I G_dGCh'C

Cuts at:      0      69     372     511     540     742     890    1025    1051 
   Size:         69     303     139      29     202     148     135      26

Cuts at:   1051    1176    1207    1304    1415    1524    1625    1682    1839 
   Size:        125      31      97     111     109     101      57     157

  Fragments arranged by size: 

                 303     202     157     148     139     135     125     111
                 109     101      97      69      57      31      29      26
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     869    1211    1839 
   Size:        869     342     628

  Fragments arranged by size: 

                 869     628     342
 
BsrBI CCG'CTC

Cuts at:      0     879    1631    1839 
   Size:        879     752     208

  Fragments arranged by size: 

                 879     752     208
 
BsrDI GCAATG_nn'

Cuts at:      0     547    1839 
   Size:        547    1292
 
BsrFI r'CCGG_y

Cuts at:      0     401     496    1658    1705    1839 
   Size:        401      95    1162      47     134

  Fragments arranged by size: 

                1162     401     134      95      47
 
BstBI TT'CG_AA

Cuts at:      0    1358    1839 
   Size:       1358     481
 
BstEII G'GTnAC_C

Cuts at:      0      25    1839 
   Size:         25    1814
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1017    1740    1839 
   Size:       1017     723      99

  Fragments arranged by size: 

                1017     723      99
 
BstYI r'GATC_y

Cuts at:      0    1032    1839 
   Size:       1032     807
 
BtgI C'CryG_G

Cuts at:      0     972    1839 
   Size:        972     867
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     877    1839 
   Size:        877     962
 
BtsI GCAGTG_nn'

Cuts at:      0      69     981    1551    1598    1839 
   Size:         69     912     570      47     241

  Fragments arranged by size: 

                 912     570     241      69      47
 
EaeI y'GGCC_r

Cuts at:      0       2      32     109     210     415     494     993    1181 
   Size:          2      30      77     101     205      79     499     188

Cuts at:   1181    1839 
   Size:        658

  Fragments arranged by size: 

                 658     499     205     188     101      79      77      30
                   2
 
EagI C'GGCC_G

Cuts at:      0     415     993    1839 
   Size:        415     578     846

  Fragments arranged by size: 

                 846     578     415
 
EarI CTCTTCn'nnn_

Cuts at:      0     931    1839 
   Size:        931     908
 
EcoICRI GAG'CTC

Cuts at:      0    1302    1839 
   Size:       1302     537
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     277     444     598     628     779     800     868 
   Size:        154     123     167     154      30     151      21      68

Cuts at:    868    1095    1111    1525    1839 
   Size:        227      16     414     314

  Fragments arranged by size: 

                 414     314     227     167     154     154     151     123
                  68      30      21      16
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1294    1839 
   Size:       1294     545
 
EcoO109I rG'GnC_Cy

Cuts at:      0     243     336    1365    1419    1520    1839 
   Size:        243      93    1029      54     101     319

  Fragments arranged by size: 

                1029     319     243     101      93      54
 
HaeII r_GCGC'y

Cuts at:      0     980    1839 
   Size:        980     859
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     814     846    1181    1213    1839 
   Size:        814      32     335      32     626

  Fragments arranged by size: 

                 814     626     335      32      32
 
KasI G'GCGC_C

Cuts at:      0     976    1839 
   Size:        976     863
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     497    1307    1839 
   Size:        497     810     532

  Fragments arranged by size: 

                 810     532     497
 
MscI TGG'CCA

Cuts at:      0       4     212    1183    1839 
   Size:          4     208     971     656

  Fragments arranged by size: 

                 971     656     208       4
 
MslI CAynn'nnrTG

Cuts at:      0     150    1408    1708    1839 
   Size:        150    1258     300     131

  Fragments arranged by size: 

                1258     300     150     131
 
MspA1I CmG'CkG

Cuts at:      0      78     606     846    1242    1380    1839 
   Size:         78     528     240     396     138     459

  Fragments arranged by size: 

                 528     459     396     240     138      78
 
NaeI GCC'GGC

Cuts at:      0     498    1660    1839 
   Size:        498    1162     179

  Fragments arranged by size: 

                1162     498     179
 
NarI GG'CG_CC

Cuts at:      0     977    1839 
   Size:        977     862
 
NgoMIV G'CCGG_C

Cuts at:      0     496    1658    1839 
   Size:        496    1162     181

  Fragments arranged by size: 

                1162     496     181
 
NspI r_CATG'y

Cuts at:      0     155     376    1272    1695    1839 
   Size:        155     221     896     423     144

  Fragments arranged by size: 

                 896     423     221     155     144
 
PasI CC'CwG_GG

Cuts at:      0    1428    1839 
   Size:       1428     411
 
PciI A'CATG_T

Cuts at:      0     151    1691    1839 
   Size:        151    1540     148

  Fragments arranged by size: 

                1540     151     148
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     219     246     358     960    1839 
   Size:        219      27     112     602     879

  Fragments arranged by size: 

                 879     602     219     112      27
 
PfoI T'CCnGG_A

Cuts at:      0     245    1839 
   Size:        245    1594
 
PmlI CAC'GTG

Cuts at:      0    1410    1839 
   Size:       1410     429
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1777    1809    1839 
   Size:       1777      32      30

  Fragments arranged by size: 

                1777      32      30
 
PpuMI rG'GwC_Cy

Cuts at:      0     243     336    1365    1419    1839 
   Size:        243      93    1029      54     420

  Fragments arranged by size: 

                1029     420     243      93      54
 
PshAI GACnn'nnGTC

Cuts at:      0     995    1839 
   Size:        995     844
 
PspOMI G'GGCC_C

Cuts at:      0    1520    1839 
   Size:       1520     319
 
PstI C_TGCA'G

Cuts at:      0    1213    1558    1839 
   Size:       1213     345     281

  Fragments arranged by size: 

                1213     345     281
 
PvuII CAG'CTG

Cuts at:      0      78     606     846    1380    1839 
   Size:         78     528     240     534     459

  Fragments arranged by size: 

                 534     528     459     240      78
 
SacI G_AGCT'C

Cuts at:      0    1304    1839 
   Size:       1304     535
 
SfcI C'TryA_G

Cuts at:      0     601    1209    1554    1839 
   Size:        601     608     345     285

  Fragments arranged by size: 

                 608     601     345     285
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1528    1839 
   Size:       1528     311
 
SfoI GGC'GCC

Cuts at:      0     978    1839 
   Size:        978     861
 
SmlI C'TyrA_G

Cuts at:      0     589     741    1198    1839 
   Size:        589     152     457     641

  Fragments arranged by size: 

                 641     589     457     152
 
StuI AGG'CCT

Cuts at:      0     626     777    1531    1839 
   Size:        626     151     754     308

  Fragments arranged by size: 

                 754     626     308     151
 
StyI C'CwwG_G

Cuts at:      0     531     540    1334    1733    1839 
   Size:        531       9     794     399     106

  Fragments arranged by size: 

                 794     531     399     106       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     155    1420    1609    1839 
   Size:        155    1265     189     230

  Fragments arranged by size: 

                1265     230     189     155
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     130     162    1839 
   Size:        130      32    1677

  Fragments arranged by size: 

                1677     130      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      90    1839 
   Size:         90    1749
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AlfI    AlwNI     ApaI    ApaLI     AvaI
    AvrII     BanI    BanII     BbeI     BbsI    BbvCI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI     EaeI     EagI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     KasI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI
     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI
     PstI    PvuII     SacI     SfcI     SfiI     SfoI     SmlI     StuI
     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AloI     ApoI     AscI     AseI    AsiSI     BaeI    BamHI
     BcgI    BciVI     BclI    BfrBI    BmgBI     BmtI    BsaBI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrGI   BssHII    BssSI   BstAPI
  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EciI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PmeI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com